Description of Leukocyt of Siam Patin Fish (Pangasius Hypophthalmus) That Feed by Addition of Harumanis Mango Seeds (Mangifera Indica L.)

Harumanis mango seed (Mangifera indica L.) is a natural ingredient that can be used as an immunostimulant. The phytochemical content in harumanis mango seeds (M. indica L.) include tannins, flavonoids, saponins and ascorbic acid. This study aims to get the best dose of adding harumanis mango seed solution (M. indica L.) to the feed. The method used was an experimental method with a Completely Randomized Design (CRD) consisting of one factor with four treatment levels which included P0 without the addition of harumanis mango seed solution, P1 addition of harumanis mango seed solution at a dose of 1.6 mL/Kg of feed, P2 addition harumanis mango seed solution at a dose of 1.8 mL/Kg of feed and P3 addition of harumanis mango seed solution at a dose of 2 mL/Kg of feed. Test fish used were Siamese catfish (P. hypophthalmus) measuring 8-12 cm which were kept in cages for 60 days. The results showed that administration of harumanis mango seed solution to feed affected the image of leukocytes of Siamese catfish (P. hypophthalmus) (P < 0,05). The best dose is in the treatment of P3 (2 mL/Kg of feed) with an average total leukocytes 9.76 x 104 cells/mm3, leukocrit levels of 1.67%, lymphocytes 82.00%, neutrophils 7.67%, monocytes 10, 33%, phagocytic activity 31.67% and absolute weight growth of 37.70 g, and 95% survival

Sensitivitas Ekstrak Daun Xylocarpus SP. terhadap Bakteri Pseudomonas SP.

This research was conducted from November 2016 until April 2017 in the Parasites and Fish Disease Laboratory, Faculty of Fisheries and Marine, University of Riau and Integrated Chemical Laboratory, Faculty of Mathematics and Natural Science of Health Muhammadiyah University of Riau. The aims of this research was to determine the sensitivity of Xylocarpus sp. leaf extract on Pseudomonas sp., the range of Minimum Inhibitory Concentration (MIC) of Xylocarpus sp. leaf extract can inhibit the growth of Pseudomonas sp. and know the toxicity of Xylocarpus sp. leaf extract against African catfish, the LC50 test conducted by immersion for 24 hours. This research used an experimental method. The treatment that being used were giving Xylocarpus sp. leaf extract at a concentration of D1 (100%), D2 (90%), D3 (80%), D4 (70%), D5 (60%), D6 (50%), D7 (40%), D8 (30%), D9 (20%), D10 (10%) and Kp : control (cloramphenicol). The results showed showed that Xylocarpus sp. leaf extract can inhibit the growth of Pseudomonas sp. at a concentration of 10% with an average of 8,12 mm zone of inhibition. MIC test showed a concentration of 8,5% average number of colonies of bacteria as much as 248,67 × 108CFU/mL can inhibit the growth of Pseudomonas sp. LC50 Xylocarpus sp. leaf extract against African catfish by immersion for 24 hours in the concentration 8,6% (860 ppm)

Erythrocyte Profile of Pangasius Hypophthalmus Feed with Rhizophora Apiculata Leaf Extract and Maintained in Net Cages

Ekstrak daun R. apiculata memiliki manfaat sebagai suplemen herbal yang dapat ditambahkan ke dalam pakan, karena mengandung senyawa flavonoid, tanin, saponin, terpenoid, dan steroid yang dapat meningkatkan kesehatan ikan jambal siam (P. hypophthalmus). Penelitian ini telah dilaksanakan pada bulan Agustus sampai Oktober 2019. Tujuan penelitian adalah untuk mendapatkan dosis terbaik ekstrak daun R. apiculata yang ditambahkan ke dalam pakan untuk meningkatkan kesehatan ikan jambal siam yang dilihat dari total eritrosit, kadar hemoglobin, kadar hematokrit, dan morfologi eritrosit. Metode yang digunakan adalah metode eksperimen dengan Rancangan Acak Lengkap (RAL)satu faktor empat taraf perlakuan terdiri dari P0;(tanpa penambahan ekstrak). P1;(1,5 mg/kg). P2;(1,7 mg/kg). dan P3;(1,9 mg/kg) dan dilakukan ulangan sebanyak tiga kali sehingga diperlukan 12 unit percobaan. Pemeliharaan dilakukan selama 60 hari di dalam keramba dengan ukuran 1x1,5x1 m3. Pemeriksaan darah dilakukan sebanyak tiga kali,yaitu pada awal pemeliharaan, hari ke-30, dan hari ke-60. Dosis terbaik terdapat pada perlakuan P2 (1,7 mg/kg) dilihat dari total eritrosit 2,57x106 sel/mm3, kadar hemoglobin 13,13 g/dL, nilai hematokrit 38,43%, pertumbuhan bobot mutlak 38,93 g, kelulushidupan 97,33%, dan menunjukkan morfologi eritrosit yang normal didukung oleh kualitas air selama penelitian seperti DO 6,4-6,9mg/L, pH 6,1-6,8, Suhu 28,2-29,5 0C, dan Amoniak 0,04-0,08 mg/L

Sensitivitas Ekstrak Buah Mengkudu (Morinda Citrifolia L.) terhadap Bakteri Streptococcus Agalactiae

The research was conducted from October to December 2014 in the Laboratory of Parasitic Diseases of Fish Faculty of Fisheries and Marine Sciences, and Laboratory of organic and natural material of Faculty of Mathematics and Natural Sciences, University of Riau, Pekanbaru. The purpose of this study to determine the concentration of noni fruit (Morinda citrifolia L.) that can inhibit the growth of Streptococcus agalactiae and safe dose to be given to the fish. The method that had been used was experimental method with concentration : P0: control, P1 (4%), P2 (5%), P3 (6%), P4 (7%), P5 (8%), P6 (9%), P7 (10%). The results show noni fruit solution to Streptococcus agalactiae sensitive average highest inhibition zone formed on treatment P7 (10%) of 9.83 mm and significantly different between treatments (P <0.05). MIC test of extract methanol Morinda citrifolia L. on treatment P2 (5%) of 78 x 108 cfu/mL. LD50 test solution for tilapia (Oreochromis niloticus) extract methanol Morinda citrifolia L. marinated for 24 hours after Reed and Muench analysis contained in the concentration of 1.42%

Pemberian Probiotik Bacillus SP Berasal Dari Udang Galah (Macrobranchium Rosenbergii De Man) Dan Udang Windu (Penaeus Monodon) Terhadap Status Kesehatan Ikan Nila(oreochormis Niloticus)

This research was conducted on October 2015 - June 2016, at the Laboratory of Microbiology and the Laboratory of Parasites and Fish Diseases, Faculty of Fisheries and Marine Sciences, University of Riau. The purpose of this study is to comprehend the probiotic Bacillus sp. from the digestive tract of the giant prawns (Macrobranchium rosenbergii DE MAN) and the black tiger shrimp (Panaeus monodon) for the health status of tilapia (Oreochormis niloticus). This study uses a Completely Randomized Design (CRD), which consists of three treatments and each of them uses three replications for the probiotic Bacillus sp. treatment of the digestive tract either from the giant prawns or the tiger shrimp. The Bacillus sp. treatment from the giant prawns are P1G (UG4 isolates), P2G isolates combined (UG1, UG2, UG3, UG4, and UG5) and as a control (P0).While the tiger shrimp are using P1W (UWH9 isolates), P2W isolates that combined (UWH1, UWH2, UWH8, UWH9 and UWH 10) and as a control (P0). Probiotics from each treatment were put into the medium water about 3 mL / container with a density of 104 CFU/ml. The results showed that the P1W treatment is the best treatment after infected by Streptococcus iniae for the health statusof tilapia, it is showed by the number of Erythrocytes 246.67x104 sel / mm, Haemoglobin level of 6633 g/dl, Hematocrytes 30.33%, and total Leukocytes 79,33x103 sel/mm3. Given by probiotics of Bacillus sp that originatit from the digestive tract of giant prawns and tiger shrimp through the water were able to maintain the health of tilapia (Oreochormis niloticus)