Isolation and Identification of Volatile Components in Teme by Simultaneous Distillation-Extraction Method by Modified Extraction Method = Isolasi dan Identifikasi Komponen Volatil Tempe dengan Metoda Distilasi-...

An isolation and identification of volatile components in tempe for 2, 5 and 8 days fermentation by simultaneous distillation-extraction method was carried out. Simultaneous distilallation-extraction apparatus was modified by Muchalal from the basic Likens-Nickerson\u27s design. Steam distillation and benzena as an extraction solvent was used in this system. The isolation was continuosly carried out for 3 hours which maximum water temperature in the Liebig condenser was 8°C. The extract was concentrated by freeze concentration method, and the volatile components were analyzed and identified by combinied gas chromatography-mass spectrophotometry (GC-MS). The Muchalal\u27s simultaneous distillation extraction apparatus have some disadvantage in cold finger condenser, and it\u27s extractor did not have condenser. At least 47, 13 and 5 volatile components was found in 2, 5 and 8 days fermentation, respectively. The volatile components in the 2 days fermentation were nonalal, a-pinene, 2,4-decadienal, 5-phenyldecane, 5- phenylundecane, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane, 5-phenyltridecane, and caryophyllenein the 5 days fermentation were nonalal, caryophyllene, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3- phenyldodecane, 2-phenyldodecaneand in the 8 days fermentation were ethenyl butanoic, 2- methyl-3-(methylethenyl)ciclohexyl etanoic and 3,7-dimethyl-5-octenyl etanoic. Keywords: Tempe, Muchalal\u27s simultaneous distillation-extraction apparatu

Isolation and Identification of Volatile Components in <i>Tempe</i> by Simultaneous Distillation-Extraction Method by Modified Extraction Method

An isolation and identification of volatile components in temps for 2, 5 and 8 days fermentation by simultaneous distillation-extraction method was carried out. Simultaneous distillation-extraction apparatus was modified by Muchalal from the basic Likens-Nickerson's design. Steam distillation and benzena as an extraction solvent was used in this system. The isolation was continuously carried out for 3 hours which maximum water temperature In the Liebig condenser was 8 °C. The extract was concentrated by freeze concentration method, and the volatile components were analyzed and identified by combined gas chromatography-mass spectrophotometry (GC-MS). The Muchalal's simultaneous distillation extraction apparatus have some disadvantage in cold finger condenser, and it's extractor did not have condenser. At least 47, 13 and 5 volatile components were found in 2, 5 and 8 days fermentation, respectively. The volatile components in the 2 days fermentation were nonalal, ɑ-pinene, 2,4-decadienal, 5-phenyldecane, 5-phenylundecane, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane, 5-phenyltridecane, and caryophyllene; in the 5 days fermentation were nonalal, caryophyllene, 4-phenylundecane, 5-phenyldodecane, 4-phenyldodecane, 3-phenyldodecane, 2-phenyldodecane; and in the 8 days fermentation were ethenyl butanoic, 2-methy1-3-(methylethenyl)ciclohexyl etanoic and 3,7-dimethyl-5-octenyl etanoic