Synthesis, characterization, and catalytic oxidation of styrene, cyclohexene, allylbenzene, and <i>cis</i>-cyclooctene by recyclable polymer-grafted Schiff base complexes of vanadium(IV)

<p>Schiff base-functionalized chloromethylated polystyrenes, PS-[Ae-Eol] (<b>I</b>), PS-[Hy-Eda] (<b>II</b>) and PS-[HyP-Eda] (<b>III</b>), were synthesized by reacting 2-(2-aminoethoxy)ethanol (Ae-Eol), N-(2-hydroxyethyl)ethylenediamine (Hy-Eda), and N-(2-hydroxpropyl)ethylenediamine (HyP-Eda) with oxidized chloromethylated polystyrene. Oxidized chloromethylated polystyrene (PS-CHO) was prepared by oxidation of chloromethylated polystyrene (PS) with sodium bicarbonate in DMSO. By reacting DMSO solution of [VO(acac)₂] with polymer-anchored Schiff base ligands <b>I</b>, <b>II</b>, and <b>III</b>, vanadium(IV) complexes PS-[V^IVO(Ae-Eol)] (<b>1</b>), PS-[V^IVO(Hy-Eda)] (<b>2</b>), and PS-[V^IVO(HyP-Eda)] (<b>3</b>) were prepared. Structure and bonding of <b>I</b>, <b>II</b>, and <b>III</b> as well as corresponding vanadium complexes <b>1</b>, <b>2</b>, and <b>3</b> were confirmed by FT-IR, UV–vis spectroscopy, SEM, EDX, AAS, TGA, EPR, <i>etc</i>. Polymer-anchored vanadium(IV) complexes <b>1</b>, <b>2</b>, and <b>3</b> show, efficient catalysis toward oxidation of styrene, cyclohexene, allylbenzene, and <i>cis</i>-cyclooctene in the presence of hydrogen peroxide. Optimized reaction conditions for the oxidation of these alkenes was achieved by changing various reaction parameters (like amount of catalyst, amount of oxidizing agent, volume of solvent, <i>etc.</i>). Polymer-grafted <b>1</b>, <b>2</b>, and <b>3</b> can be reused multiple times without depletion of their activity.</p

Comparative Analysis of Tocopherol Biosynthesis Genes and Its Transcriptional Regulation in Soybean Seeds

Tocopherols composed of four isoforms (α, β, γ, and δ) and its biosynthesis comprises of three pathways: methylerythritol 4-phosphate (MEP), shikimate (SK) and tocopherol-core pathways regulated by 25 enzymes. To understand pathway regulatory mechanism at transcriptional level, gene expression profile of tocopherol-biosynthesis genes in two soybean genotypes was carried out, the results showed significantly differential expression of 5 genes: 1-deoxy-d-xylulose-5-P-reductoisomerase (DXR), geranyl geranyl reductase (GGDR) from MEP, arogenate dehydrogenase (TyrA), tyrosine aminotransferase (TAT) from SK and γ-tocopherol methyl transferase 3 (γ-TMT3) from tocopherol-core pathways. Expression data were further analyzed for total tocopherol (T-toc) and α-tocopherol (α-toc) content by coregulation network and gene clustering approaches, the results showed least and strong association of γ-TMT3/tocopherol cyclase (TC) and DXR/DXS, respectively, with gene clusters of tocopherol biosynthesis suggested the specific role of γ-TMT3/TC in determining tocopherol accumulation and intricacy of DXR/DXS genes in coordinating precursor pathways toward tocopherol biosynthesis in soybean seeds. Thus, the present study provides insight into the major role of these genes regulating the tocopherol synthesis in soybean seeds

Comparative Analysis of Tocopherol Biosynthesis Genes and Its Transcriptional Regulation in Soybean Seeds

Tocopherols composed of four isoforms (α, β, γ, and δ) and its biosynthesis comprises of three pathways: methylerythritol 4-phosphate (MEP), shikimate (SK) and tocopherol-core pathways regulated by 25 enzymes. To understand pathway regulatory mechanism at transcriptional level, gene expression profile of tocopherol-biosynthesis genes in two soybean genotypes was carried out, the results showed significantly differential expression of 5 genes: 1-deoxy-d-xylulose-5-P-reductoisomerase (DXR), geranyl geranyl reductase (GGDR) from MEP, arogenate dehydrogenase (TyrA), tyrosine aminotransferase (TAT) from SK and γ-tocopherol methyl transferase 3 (γ-TMT3) from tocopherol-core pathways. Expression data were further analyzed for total tocopherol (T-toc) and α-tocopherol (α-toc) content by coregulation network and gene clustering approaches, the results showed least and strong association of γ-TMT3/tocopherol cyclase (TC) and DXR/DXS, respectively, with gene clusters of tocopherol biosynthesis suggested the specific role of γ-TMT3/TC in determining tocopherol accumulation and intricacy of DXR/DXS genes in coordinating precursor pathways toward tocopherol biosynthesis in soybean seeds. Thus, the present study provides insight into the major role of these genes regulating the tocopherol synthesis in soybean seeds