Activation of p34cdc2 kinase by cyclin A

Functional clam cyclin A and B proteins have been produced using a baculovirus expression system. Both cyclin A and B can induce meiosis I and meiosis II in Xenopus in the absence of protein synthesis. Half-maximal induction occurs at 50 nM for cyclin A and 250 nM for cyclin B. Addition of 25 nM cyclin A to activated Xenopus egg extracts arrested in the cell cycle by treatment with RNase or emetine activates cdc2 kinase to the normal metaphase level and stimulates one oscillatory cell cycle. High levels of cyclin A cause marked hyperactivation of cdc2 kinase and a stable arrest at the metaphase point in the cell cycle. Kinetic studies demonstrate the concentration of cyclin A added does not affect the 10 min lag period required for kinase activation or the timing of maximal activity, but does control the rate of deactivation of cdc2 kinase during exit from mitosis. In addition, exogenous clam cyclin A inhibits the degradation of both A- and B-type endogenous Xenopus cyclins. These results define a system for investigating the biochemistry and regulation of cdc2 kinase activation by cyclin A

A hypothetico-deductive approach to assessing the social function of chemical signalling in a non-territorial solitary carnivore

The function of chemical signalling in non-territorial solitary carnivores is still relatively unclear. Studies on territorial solitary and social carnivores have highlighted odour capability and utility, however the social function of chemical signalling in wild carnivore populations operating dominance hierarchy social systems has received little attention. We monitored scent marking and investigatory behaviour of wild brown bears Ursus arctos, to test multiple hypotheses relating to the social function of chemical signalling. Camera traps were stationed facing bear ‘marking trees’ to document behaviour by different age sex classes in different seasons. We found evidence to support the hypothesis that adult males utilise chemical signalling to communicate dominance to other males throughout the non-denning period. Adult females did not appear to utilise marking trees to advertise oestrous state during the breeding season. The function of marking by subadult bears is somewhat unclear, but may be related to the behaviour of adult males. Subadults investigated trees more often than they scent marked during the breeding season, which could be a result of an increased risk from adult males. Females with young showed an increase in marking and investigation of trees outside of the breeding season. We propose the hypothesis that females engage their dependent young with marking trees from a young age, at a relatively ‘safe’ time of year. Memory, experience, and learning at a young age, may all contribute towards odour capabilities in adult bears

SWCam: the short wavelength camera for the CCAT Observatory

We describe the Short Wavelength Camera (SWCam) for the CCAT observatory including the primary science drivers, the coupling of the science drivers to the instrument requirements, the resulting implementation of the design, and its performance expectations at first light. CCAT is a 25 m submillimeter telescope planned to operate at 5600 meters, near the summit of Cerro Chajnantor in the Atacama Desert in northern Chile. CCAT is designed to give a total wave front error of 12.5 μm rms, so that combined with its high and exceptionally dry site, the facility will provide unsurpassed point source sensitivity deep into the short submillimeter bands to wavelengths as short as the 200 μm telluric window. The SWCam system consists of 7 sub-cameras that address 4 different telluric windows: 4 subcameras at 350 μm, 1 at 450 μm, 1 at 850 μm, and 1 at 2 mm wavelength. Each sub-camera has a 6’ diameter field of view, so that the total instantaneous field of view for SWCam is equivalent to a 16’ diameter circle. Each focal plane is populated with near unit filling factor arrays of Lumped Element Kinetic Inductance Detectors (LEKIDs) with pixels scaled to subtend an solid angle of (λ/D)2 on the sky. The total pixel count is 57,160. We expect background limited performance at each wavelength, and to be able to map < 35(°)2 of sky to 5 σ on the confusion noise at each wavelength per year with this first light instrument. Our primary science goal is to resolve the Cosmic Far-IR Background (CIRB) in our four colors so that we may explore the star and galaxy formation history of the Universe extending to within 500 million years of the Big Bang. CCAT's large and high-accuracy aperture, its fast slewing speed, use of instruments with large format arrays, and being located at a superb site enables mapping speeds of up to three orders of magnitude larger than contemporary or near future facilities and makes it uniquely sensitive, especially in the short submm bands

MAKO: a pathfinder instrument for on-sky demonstration of low-cost 350 micron imaging arrays

Submillimeter cameras now have up to 10^4 pixels (SCUBA 2). The proposed CCAT 25-meter submillimeter telescope will feature a 1 degree field-of-view. Populating the focal plane at 350 microns would require more than 10^6 photon-noise limited pixels. To ultimately achieve this scaling, simple detectors and high-density multiplexing are essential. We are addressing this long-term challenge through the development of frequency-multiplexed superconducting microresonator detector arrays. These arrays use lumped-element, direct-absorption resonators patterned from titanium nitride films. We will discuss our progress toward constructing a scalable 350 micron pathfinder instrument focusing on fabrication simplicity, multiplexing density, and ultimately a low per-pixel cost

Continental mass change from GRACE over 2002-2011 and its impact on sea level

Present-day continental mass variation as observed by space gravimetry reveals secular mass decline and accumulation. Whereas the former contributes to sea-level rise, the latter results in sea-level fall. As such, consideration of mass accumulation (rather than focussing solely on mass loss) is important for reliable overall estimates of sea-level change. Using data from the Gravity Recovery And Climate Experiment satellite mission, we quantify mass-change trends in 19 continental areas that exhibit a dominant signal. The integrated mass change within these regions is representative of the variation over the whole land areas. During the integer 9-year period of May 2002 to April 2011, GIA-adjusted mass gain and mass loss in these areas contributed, on average, to −(0.7 ± 0.4) mm/year of sea-level fall and + (1.8 ± 0.2) mm/year of sea-level rise; the net effect was + (1.1 ± 0.6) mm/year. Ice melting over Greenland, Iceland, Svalbard, the Canadian Arctic archipelago, Antarctica, Alaska and Patagonia was responsible for + (1.4±0.2) mm/year of the total balance. Hence, land-water mass accumulation compensated about 20 % of the impact of ice-melt water influx to the oceans. In order to assess the impact of geocentre motion, we converted geocentre coordinates derived from satellite laser ranging (SLR) to degree-one geopotential coefficients. We found geocentre motion to introduce small biases to mass-change and sea-level change estimates; its overall effect is + (0.1 ± 0.1) mm/year. This value, however, should be taken with care owing to questionable reliability of secular trends in SLR-derived geocentre coordinates