Factors Affecting Incidence of Uterine Torsion in Egyptian Buffaloes (Bubalus bubalis) and its Response for Rolling

Objective: The present work was planned to study the incidence of uterine torsion in Egyptian buffaloes as well as its prognosis after mechanical treatment.Methods: A total number of 35 buffaloes of different ages, parities and stage of pregnancy with complain of colic and anorexia were included in the present study. These animals were examined rectally to detect stage of pregnancy, degree, direction, duration, location of uterine torsion. Mechanical handling of torsion cases and prognosis of recovered cases was performed. After birth, sex of new born was also recorded.Results: The torsion of uterus mostly occurred in pleuriparous buffaloes (100%) in right side (85.7%) during second half of pregnancy (100%). Most cases were postcervical (85.7%) during the early stage (7 and 8 months). Torsion also may occur during labour (17.4%) where the cervix was dilated after correction. Most cases of uterine torsion occur in stabled animals for long period (97%). The uterine torsion associated with male calves (65.7%) as well as with large size calves (40-50kg) and majority of the calves were in normal position. The first foetal sac expelled in majority of cases was amniotic sac (97%) with few cases of placental retention. Uterine torsion with short duration (1-6 h) usually needs 1-2 rolls only for correction and usually takes 0-6h (100%) for cervical dilatation. Occurrence of uterine torsion for above three days usually did not respond for rolling.Conclusion: Uterine torsion usually occurs in old stabled animal with good BCS during green season at last month of pregnancy. The right sever and post cervical torsion were the most common type of torsion. Rapid diagnosis of uterine torsion within 6 h usually results in good prognosis even in severe cases

Inactivated pentavalent vaccine against mycoplasmosis and salmonellosis for chickens

Mycoplasma and Salmonella are serious pathogens threaten the poultry industry. This study aimed to prepare and evaluate an inactivated pentavalent vaccine targeting bacteria, including Salmonella enterica serovar Typhimurium (ST), Salmonella enterica serovar Enteritidis (SE), Salmonella enterica serovar Kentucky (SK), Mycoplasma gallisepticum (MG), and Mycoplasma synoviae (MS), from locally isolated strains. The prepared vaccine was adjuvanted with Montanide ISA70 oil and then tested for safety, sterility, and potency. The vaccine efficacy was evaluated in 110 specific pathogen-free, 1-day-old chicks, which were divided into three groups as follows: 1) vaccinated group (50 birds), which was subdivided into five subgroups of ten birds each; 2) control positive (challenged) group (50 birds), which was subdivided into five subgroups of ten birds each; and 3) control negative (blank) group, which included ten birds. Chicks in group 1 were administered the first dose of vaccine at 7 d of age followed by a booster dose after 3 wk. At 3 wk after booster vaccination, the chicks who were administered the booster dose were challenged and kept under observation until the end of the experiment when the chicks were approximately 10 wk. Details of clinical symptoms, daily mortality, weights, and postmortem lesions; serum samples; cloacal swabs; and nasal swabs were collected during the experiment. The humoral immune response to the prepared pentavalent vaccine was assessed using enzyme-linked immunosorbent assay. Our findings revealed that the prepared vaccine showed high protective antibody titers against Salmonella and Mycoplasma with 100% efficacy and no mortalities (100% survival rate) were recorded in vaccinated and challenged birds. The vaccine reduced both clinical signs and bacterial shedding post challenge in vaccinated birds in comparison with control positive group. The prepared vaccine did not affect the body weight gain of the vaccinated birds in comparison with control negative birds. The current study concluded that locally manufactured inactivated pentavalent vaccine offers protection to birds and could be employed as an effective tool along with biosecurity measures to overcome mycoplasmosis and salmonellosis in layer and breeder chicken farms in Egypt

Impacts of tea tree or lemongrass essential oils supplementation on growth, immunity, carcass traits, and blood biochemical parameters of broilers reared under different stocking densities

The effects of tea tree essential oil (TTEO) and lemongrass essential oil (LGEO) with different stocking densities on the growth performance, biochemical markers, antioxidants, and immunity state of broiler chickens were studied. Birds were housed at stocking densities of 25, 30, 35, and 40 kg/m2. The treatments were, basal diet without any supplementation, the second and third groups were supplemented with 300 mg TTEO/kg feed, and 300 mg LGEO/kg feed, respectively. Results revealed that increasing stocking density from 25 to 40 kg/m2 significantly reduced body weight and daily weight gain at different ages. The phagocytic index and activity were significantly higher under the lower stocking density (25 kg/m2). Serum amyloid A (SAA), serum or liver transferrin (TRF), or C-reactive protein (CRP) were significant decreased when decreasing stocking density. Increasing stocking density from 25 to 40 kg/m2 resulted in a significant increase in the serum urea, creatinine, uric acid, lactate dehydrogenase (LDH), alanine aminotransferase (ALT), aspartate aminotransferase (AST), malondialdehyde (MDA), and catalase (CAT) levels. However, there was a significant reduction in antioxidant enzyme activity, including glutathione peroxidase (GPx) and superoxide dismutase (SOD), as stocking density increased. The supplementation of TTEO produced significantly higher body weight and daily weight gain followed by LGEO. Additionally, the mortality rates were reduced in TTEO (27.4%) and LGEO (25%) groups. TTEO or LGEO supplementation significantly improved meat constituents and cellular immunity and reduced serum total lipids, serum and meat cholesterol, and triglycerides, SAA, TRF, and CRP. For all these measured parameters, superior results were obtained when TTEO was used compared to LGEO. TTEO or LGEO supplementation also significantly reduced serum urea, creatinine, uric acid, and the enzymatic activities of LDH, ALT, AST, MDA, and CAT (but not GPx and SOD) in comparison to the control treatment. Overall, our results showed the superiority of TTEO over LGEO as a feed supplement in broiler diets. In conclusion, TTEO treatment offers a better solution for raising broiler chickens in high stocking density

Impacts of supplementing broiler diets with a powder mixture of black cumin, Moringa and chicory seeds

The present study aimed at evaluating the effect of a phytogenic mixture in the diet on broiler production. A total of 400 day-old unsexed Cobb broiler chicks were randomly allotted to four treatment groups of 10 replications in a randomised design experiment. The phytogenic feed mixture (BMC) contained equal ratios of black cumin, Moringa oleifera and chicory seeds. The treatment groups were as follows: T1 was fed the basal diet, while T2, T3 and T4 were fed the basal diet supplemented with 0.2%, 0.4% or 0.6% of three BMC mixture, respectively. Results showed that increasing the dietary BMC level could be associated with a gradual but significant increase in body weight and improvement in the feed conversion ratio when compared with the control group. Broiler diets enriched with 0.4% to 0.6% of the BMC mixture reduced gut microbial count of coliforms, E. coli and C. perfringens as well as gut pH, compared to the control group. Increasing the dietary BMC mixture level was associated with gradual but significant decrease in serum total cholesterol, low density lipoprotein concentrations and liver enzymes concentrations. However, there was an increase in the high density lipoprotein concentration, and glutathione peroxidase and superoxide dismutase activity in serum. In conclusion, the BMC mixture could be deemed an effective growth promoter, but further research is needed to evaluate it as a viable alternative to antibiotics.Keywords: blood, broiler, carcass, microbiota, oxidative status, performance, phytogeni

Effects of phytogenic feed additives on the reproductive performance of animals

The reproductive performance of ruminants is economically significant, and its improvement is a primary goal of the livestock industry to ensure its sustainability. Several approaches have been developed to use phytogenics as feed additives for several proposes, such as reducing methane emissions, and as an alternative to antibiotics. Phytogenics have potent antioxidant, anti-inflammatory, immunomodulatory, and metabolism-regulatory properties, and they are present at high levels in animal feeds. This current review considers the potential use of medicinal herbs on the reproductive performance of animals. The influence of diet on the fertility complications commonly noted in ruminants is of global interest. Although the effects of phytogenics on ruminant digestion and absorption are well-explored, their impact on reproductive performance remains poorly investigated. This review focuses on the influence of phytogenics on semen quality, hormonal profiles, and hematobiochemical indices in male ruminants. Based on available data, phytogenics are perceived to improve oocyte quality, reproductive performance, and pregnancy. However, further more comprehensive research on the benefits and potential hazards of the use of phytogenics is required to improve reproductive performance in ruminants

Impacts of dietary inclusion of dried brewers’ grains on growth, carcass traits, meat quality, nutrient digestibility and blood biochemical indices of broilers

The current investigation aimed to evaluate the effect of dietary inclusion of dried brewers’ grains (DBG) on growth performance, carcass traits, meat quality, nutrient digestibility, blood biochemical constituents and antioxidant indices of broiler chickens. A total of 300 unsexed one-week-old Hubbard chicks were randomly allotted to five treatment groups. The dietary treatments consisted of a basal diet as the control and DBG groups (3%, 6%, 9% and 12%, respectively). The best feed conversion ratio (FCR) was found in the group of birds fed a diet containing 9% DBG, compared the other groups. Dressing and abdominal fat percentages decreased significantly but gradually with the elevated DBG level in the diets from 6% to 12%. The inclusion of DBG in broiler diets resulted in significant positive effects on all of the sensory evaluation indicators except appearance and tenderness. Broilers fed a diet containing 3% DBG had significant higher of digestion coefficients for crude protein than those fed on the other experimental diets. Based on growth performance and health status, 3% or 9% DBG may be the optimum percentages for inclusion in the diets of poultry until six weeks old. In addition, DBG exerted several health benefits in meat, which would be reflected positively on human health.Keywords: unconventional feedstuff, inclusion, performance, chicken, meat qualit

Pharmacological, nutritional and antimicrobial uses of Moringa oleifera Lam. leaves in poultry nutrition: An updated knowledge

Recently, developing countries have focused on using innovative feed in poultry nutrition. The plant Moringa oleifera is native to India but grows worldwide in tropical and subtropical climates. Moringa is planted on a large scale as it can tolerate severe dry and cold conditions. All parts of this plant can be used for commercial or nutritional purposes, and it has a favorable nutritional profile. Beneficial phytochemicals, minerals, and vitamins are abundant in the leaves. The leaf extracts can be used to treat malnutrition; they also possess anticancer, antioxidant, antidiabetic, antibacterial, and anti-inflammatory properties. Further, moringa contains antinutritional substances, such as trypsin inhibitors, phytates, tannins, oxalates, cyanide, and saponins, which have a harmful effect on mineral and protein metabolism. Previous research suggested that including moringa in chicken diets boosts their growth and productivity. Therefore, this review focuses on the characterization and application of M. oleifera in poultry nutrition and its potential toxicity. Furthermore, we discuss the nutritional content, phytochemicals, and antioxidants of M. oleifera leaf meal and its applicability in poultry rations

The relationship among avian influenza, gut microbiota and chicken immunity: An updated overview

The alimentary tract in chickens plays a crucial role in immune cell formation and immune challenges, which regulate intestinal flora and sustain extra-intestinal immunity. The interaction between pathogenic microorganisms and the host commensal microbiota as well as the variety and integrity of gut microbiota play a vital role in health and disease conditions. Thus, several studies have highlighted the importance of gut microbiota in developing immunity against viral infections in chickens. The gut microbiota (such as different species of Lactobacillus, Blautia Bifidobacterium, Faecalibacterium, Clostridium XlVa, and members of firmicutes) encounters different pathogens through different mechanisms. The digestive tract is a highly reactive environment, and infectious microorganisms can disturb its homeostasis, resulting in dysbiosis and mucosal infections. Avian influenza viruses (AIV) are highly infectious zoonotic viruses that lead to severe economic losses and pose a threat to the poultry industry worldwide. AIV is a challenging virus that affects gut integrity, disrupts microbial homeostasis and induces inflammatory damage in the intestinal mucosa. H9N2 AIV infection elevates the expression of proinflammatory cytokines, such as interferon (IFN-γ and IFNα) and interleukins (IL-17A and IL-22), and increases the proliferation of members of proteobacteria, particularly Escherichia coli. On the contrary, it decreases the proliferation of certain beneficial bacteria, such as Enterococcus, Lactobacillus and other probiotic microorganisms. In addition, H9N2 AIV decreases the expression of primary gel-forming mucin, endogenous trefoil factor family peptides and tight junction proteins (ZO-1, claudin 3, and occludin), resulting in severe intestinal damage. This review highlights the relationship among AIV, gut microbiota and immunity in chicken

Phytochemical control of poultry coccidiosis: A review

Avian coccidiosis is a major parasitic disorder in chickens resulting from the intracellular apicomplexan protozoa Eimeria that target the intestinal tract leading to a devastating disease. Eimeria life cycle is complex and consists of intra- and extracellular stages inducing a potent inflammatory response that results in tissue damage associated with oxidative stress and lipid peroxidation, diarrheal hemorrhage, poor growth, increased susceptibility to other disease agents, and in severe cases, mortality. Various anticoccidial drugs and vaccines have been used to prevent and control this disorder; however, many drawbacks have been reported. Drug residues concerning the consumers have directed research toward natural, safe, and effective alternative compounds. Phytochemical/herbal medicine is one of these natural alternatives to anticoccidial drugs, which is considered an attractive way to combat coccidiosis in compliance with the “anticoccidial chemical-free” regulations. The anticoccidial properties of several natural herbal products (or their extracts) have been reported. The effect of herbal additives on avian coccidiosis is based on diminishing the oocyst output through inhibition or impairment of the invasion, replication, and development of Eimeria species in the gut tissues of chickens; lowering oocyst counts due to the presence of phenolic compounds in herbal extracts which reacts with cytoplasmic membranes causing coccidial cell death; ameliorating the degree of intestinal lipid peroxidation; facilitating the repair of epithelial injuries; and decreasing the intestinal permeability induced by Eimeria species through the upregulation of epithelial turnover. This current review highlights the anticoccidial activity of several herbal products, and their other beneficial effects

Promising prospective effects of Withania somnifera on broiler performance and carcass characteristics: A comprehensive review

Poultry production contributes markedly to bridging the global food gap. Many nations have limited the use of antibiotics as growth promoters due to increasing bacterial antibiotic tolerance/resistance, as well as the presence of antibiotic residues in edible tissues of the birds. Consequently, the world is turning to use natural alternatives to improve birds' productivity and immunity. Withania somnifera, commonly known as ashwagandha or winter cherry, is abundant in many countries of the world and is considered a potent medicinal herb because of its distinct chemical, medicinal, biological, and physiological properties. This plant exhibits antioxidant, cardioprotective, immunomodulatory, anti-aging, neuroprotective, antidiabetic, antimicrobial, antistress, antitumor, hepatoprotective, and growth-promoting activities. In poultry, dietary inclusion of W. somnifera revealed promising results in improving feed intake, body weight gain, feed efficiency, and feed conversion ratio, as well as reducing mortality, increasing livability, increasing disease resistance, reducing stress impacts, and maintaining health of the birds. This review sheds light on the distribution, chemical structure, and biological effects of W. somnifera and its impacts on poultry productivity, livability, carcass characteristics, meat quality, blood parameters, immune response, and economic efficiency