20 research outputs found
In-Vitro Anti-oxidant And Antimicrobial Study of Ficus Hispida
Ficus hispida L. belongs to the Moraceae family and is used by the maaiba trible (indigenous medicine - man of Manipur, India) as an indigenous traditional medicine. Present study deals with the successive extraction of the aerial parts of Ficus hispida and in-vitro screening of anti-oxidant and anti-microbial activity. The phytochemical screening of the methanol extract of Ficus hispida shows the presence of secondary metabolite groups like alkaloid, phenolic compounds, flavonoid, glycosides, protein etc. Phenolic compounds are commonly found in both edible and nonedible plants and are responsible for various medicinal activities of plants, so our study is based on determining antioxidant activity and anti-microbial activity. Beside these, we also measured the total flavonoid and total phenolic content of the respective sample to understand the effect of polyphenolic compound on different pathophysiological state associated with high free radical production. The in-vitro investigation proves the efficiency of this plant in various diseases states
In Silico Molecular Modeling and Structural Analysis of Peroxidase Enzymes from five different plants species
ABSTRACT Upon attacked by pathogens, plants defend themselves by producing array of defense related molecules. Plant peroxidase enzymes are about 300-350 residue long and have multiple isoenzymes that differ in substrate specificity and localization within the plant. In this study, 3D structure of peroxidase enzymes from five different plants was predicted by homology modeling method. The quality of the 3D structure of the model was confirmed by various web based validation programs. When compared secondary and tertiary structure of the model, it showed two peroxidase signature domains (PEROXIDASE_1 and PEROXIDASE_2) are present in the central region. Degree of sequence conserveness showed that sequence size differences do not make any impact on their basic functions
Social choice: locating public facilities & voting in a large electorate: two location problems and a voting problem
Frequency based analysis of collective aggregation rules
A new property for collective aggregation rules called positive discrimination is introduced. This property is satisfied by many anonymous and neutral collective aggregation rules. We discuss unimodal profiles (or distributions) for which this property on its own determines the outcome as the mode of a unimodal distribution. We investigate relaxations of this unimodal distribution condition and show that the Condorcet consistent rules as well as the Borda rule and the plurality rule have the mode as the outcome at such profiles. Further, we show, by an example, how the addition of unimodal profiles and the property of positive discrimination can help to determine an outcome in the case of a large number of voters. (C) 2020 Elsevier B.V. All rights reserved
Locating a public good on a sphere
It is shown that in a model where agents have single-peaked preferences on the sphere, every Pareto optimal social choice function that is strict or coalitional strategy-proof, is dictatorial
Honey Extracted Polyphenolics Reduce Experimental Hypoxia in Human Keratinocytes Culture
Hypoxic
assault affects fundamental cellular processes and generates
oxidative stress on healthy cells/molecules. Honey extracted polyphenolics
(HEP) as a natural antioxidant reduced hypoxic cytotoxicity in this
study. Different honey samples were physicochemically characterized
to identify preferred (jamun) honey [pH 3.55 ± 0.04, conductivity
(μs/cm) = 6.66 ± 0.14, water content % (w/w) = 14.70 ±
0.35, total solid content % (w/w) = 85.30 ± 0.35, phenol content
(mg GAE/100 g) = 403.55 ± 0.35, flavonoid content (mg QE/100
g) = 276.76 ± 4.10, radical scavenging activity (% 500 μL)
= 147.75 ± 3.13, catalase activity (absorbance at 620 nm) = 0.226
± 0.01]. HEP was tested in different doses on hypoxic and normoxic
cells (HaCaT) using viability and antioxidant assays. Cardinal molecular
expressions such as cadherin–catenin–cytoskeleton complex
(namely, E-cadherin, β-catenin, and F-actin), hypoxia marker
(Hif 1 α), proliferation marker (Ki67), and epithelial master
regulator (p63) were studied by immuno-cytochemisty (ICC) and qRT-PCR.
The 0.063 mg/mL HEP demonstrated better vitality and functionality
of HaCaT cells as per viability assay (*, <i>P</i> <
0.01) even under hypoxia. ICC and qRT-PCR observations indicated restoration
of cellular survival and homeostasis under 0.063 mg/mL HEP after hypoxic
assault. Furthermore, major spectral changes for nucleic acid and
membrane phospholipid reorganizations by Fourier transform infrared
spectroscopy illustrated a positive impact of 0.063 mg/mL HEP on hypoxic
cells considering proliferation and cellular integrity. It was concluded
that a specific dose of jamun HEP reduces hypoxic cytotoxicity