The epigenetic control of E-box and Myc-dependent chromatin modifications regulate the licensing of lamin B2 origin during cell cycle

Recent genome-wide mapping of the mammalian replication origins has suggested the role of transcriptional regulatory elements in origin activation. However, the nature of chromatin modifications associated with such trans-factors or epigenetic marks imprinted on cis-elements during the spatio-temporal regulation of replication initiation remains enigmatic. To unveil the molecular underpinnings, we studied the human lamin B2 origin that spatially overlaps with TIMM 13 promoter. We observed an early G1-specific occupancy of c-Myc that facilitated the loading of mini chromosome maintenance protein (MCM) complex during subsequent mid-G1 phase rather stimulating TIMM 13 gene expression. Investigations on the Myc-induced downstream events suggested a direct interaction between c-Myc and histone methyltransferase mixed-lineage leukemia 1 that imparted histone H3K4me3 mark essential for both recruitment of acetylase complex HBO1 and hyperacetylation of histone H4. Contemporaneously, the nucleosome remodeling promoted the loading of MCM proteins at the origin. These chromatin modifications were under the tight control of active demethylation of E-box as evident from methylation profiling. The active demethylation was mediated by the Ten-eleven translocation (TET)-thymine DNA glycosylase-base excision repair (BER) pathway, which facilitated spatio-temporal occupancy of Myc. Intriguingly, the genome-wide 43% occurrence of E-box among the human origins could support our hypothesis that epigenetic control of E-box could be a molecular switch for the licensing of early replicating origins

A Casual Study on Impact of Green HRM Practices on Organisation Sustainability

Nowadays, our business environment has changed itsview from a traditional financial perspective tocompetency based and strategic based perspective. Thegreen movement across the world has given birth to theconcept of Green HRM. The concept became popularowing to the issues in the present business world, rangingfrom excessive consumption of natural resources, carboncredits, global warming, to pollution. All these results inserious industrial accidents decrease in life span, changesin climate, etc. In general, Green HR deals with practicingall HR activities with environment-friendly concerns.This, in turn, helps in the sustainability of businesses aswell as the employees. Nevertheless, there is a wide crackbetween the HRM and environmental facets. Thereforethere is an emerging requirement for research inarticulating green HR approaches and their suggestionson general business execution. The reason behind thisinvestigation is to talk about the ideas of green HRM andits impact on organisation sustainability with the help ofextensive review of the literature

Relationship of Prestrengthening Exercise before Tournament for the Performance of Female Wheel Chair Basketball Players in Free Throw

INTRODUCTION: Cross-sectional studies have reported that a majority of long-term wheelchair users experience upper extremity pain. Overuse and repetitive stress often result in degenerative soft tissue changes it been observed that 18% of active persons and 45% of inactive persons with long-term paraplegia also showed degenerative changes in the shoulder joint on radiographs. Other researchers have reported that wheelchair users often show chronic degenerative injuries to soft tissues, including impingement syndromes, rotator cuff tears, sprains, strains, and avascular necrosis AIM OF THE STUDY: To find out the effect of Strength Training, reducing the pain & performance of Upper limb for Wheelchair Basketball Players. Thus it provides a model for the technique of skill for analyzing the performance of the players. Which will be helpful in preparing how effectively the free throw and shooting and set shot has made. OBJECTIVE: To determine in performing the shooting the ball with the help of upper extremities of female wheelchair basket ball players. METHODOLOGY: Study design: Comparitive study. Study setting: The study was done in 15 wheel chair players in Chennai Source of data: Data was collected from 15 private players in Chennai. All subjects were assessed and selected based upon who fulfilled the inclusion criteria. The purpose of the study was explained to all subjects and consent from each subject was taken. The subjects were randomly assigned into the either Pre Strengthening Training (Group A) and Simple Training (Group B). Sample size: 15 subjects Inclusion Criteria: Impaired muscle power, Impaired passive range of movement, Hypertonia, Limb deficiency, Ataxia, Leg length difference, Short stature. Exclusion Criteria: Foot ulcers, Vision impairment. DATA ANALYSIS AND INTERPRETATION: This deals with the analysis of data collected from the subjects under study. The primary concern of the present study was to find out significance relationship between the selected pre strengthening exercise and the performance of the subjects in the free throw and shooting the ball analyzing among the female wheelchair from Chennai team. To facilitate the study, 15 shoulder pain female wheelchair basketball players were randomly selected for the purpose of this study. Their age was between 24 to 28 years. RESULTS: After give 6 weeks Pre – Strengthening Shoulder Exercise, it’s been proved that the players where playing their game without the pain on their shoulder while passing, shooting , free throws. In table 1 & graph 1, the pre test values were compared with post test values of Free throw, Long Pass, Dribbling of group A players after Pre – Strengthening exercise. According to this table p value is less than .05 which shows that there is a significant difference in tournament in group A who underwent Pre – strengthening training. In table 2 & graph 2, the pre test values were compared with post test values of Free throw, Long Pass, Dribbling of group B players trained on ground. According to this table p - value is more than .05 which shows that there is a no significant difference in shoulder pain in group B who underwent basic training in ground. Thus player in Group A where able to perform better than the Players in Group B after the 6 – weeks Pre – Strengthening exercise Program. CONCLUSION: The results obtained from this study shows significant difference for players trained under pre – strengthening program before the tournament .The mean difference for free throw, long pass , dribbling showed significant increase in the group – A compared with group - B. From the above result it can be concluded that six weeks pre - strenthening training is more effective in improving the performance in the tournament the Female wheelchair basketball players. Hence it can be recommended that 6 weeks pre – strengthening training is more effective, useful & performance oriented rather than the usual training program

Detect and Classify the Unpredictable Cyber-Attacks by using DNN Model

Machine learning techniques are often used to develop IDS by detecting and deploying fast and automated network attacks to torpedoes and host standards. However, there are many problems, as severe attacks change all the time and occur at very high levels that require a lot of resolution. There are many malicious packages available for further investigation by the cybersecurity community. However, one completed study did not provide a complete analysis to apply different machine learning algorithms on different media packages. Because of the persistent methods of attack and the dynamic nature of malware, it is important to systematically update and approve malicious packages that are available to the public. This paper explores the DNN, a type of comprehensive learning model, promoting flexible and appropriate IDS for detecting and deploying expected and unpredictable online attacks. Sustainable industrial development and rapid development of attacks need evaluation for some data developed over the years using static and dynamic methods. This type of research can help determine the best algorithm to identify future attacks. Comparative data for some commonly available malware provides a comprehensive comparison of DNN experiences with other class machine learning classifications. The best network parameters and network topologies for DNN are selected using the KDDCup 99 package with this hyperparameter selection method. The DNN model, which works well on KDDCup 99, works on other data, such as the NSL-KDD memory test. Our DNN model teaches how to transfer IDS information functions from multicultural.Multidisciplinary representations in a variety of encryption. Complex tests have shown that DNN performs better than conventional machine learning classification. Finally, we present a large and hybrid DNN torrent structure called Scale-Hybrid-IDS-AlertNet, which can be used to effectively monitor the impact of network traffic and host-level events to warn directly about cyber-attacks

“Do you think it is a Pandemic?” Apocalypse, Anxiety and the Environmental Grotesque in Paolo Bacigalupi’s The Windup Girl

Paolo Bacigalupi’s The Windup Girl (2009), set in the post fossil-fuel, post turbo-capitalist country of Thailand, portrays the shocking after effects of bioengineering and gene-hack modifications in food crops. The narrative depicts a country tottering on the brink of an agricultural apocalypse on account of food production being severely affected by crop driven anomalies and rogue diseases such as “cibiscosis” and “blister rust” transmitted by variants of mutating pests. Natural seed stock becomes completely supplanted by the new genetically engineered seeds which become sterile after a single seasonal cycle of sowing and harvesting. The native population of Thailand is adversely affected by the pandemic scenario, which becomes aggravated by an expedient “scientocracy” that is at the heart of the neocolonial enterprises of American megacorporations and calorie companies like Agrigen, PurCal and Redstar who hail gene hacking as the new future of food resources and market profiteering. The consumption of the gene-hacked produce spreads through crops and affects the human body in unimaginable ways thereby resulting in a considerable rise of health issues including digestive and respiratory failures. This paper intends to articulate the idea of a pandemic, its historical understanding and affective influences in the context of a post techno-fossil fuel economy set in Thailand. It will analyse the idea of epidemiological colonialism; diseases introduced by colonising forces that reshape the natives’ existing environment thereby bringing forth a deep pandemic anxiety that percolates the collective memory of the Thai people. It also highlights how the novel portrays the conflict between traditional ecological knowledge systems and modern extractive enterprises that acts as a catalyst to hasten the destruction of sustainable systems of agriculture and food production that have endured the impact of climate change and ecological fallout. The paper will study the relevance of the pandemic as an agency of ecocatastrophe and its function in an eco-speculative science fictional narrative. Finally, the paper looks into the concept of the posthuman android, genetically modified humans in a “technologiade”, a society reconfigured by technoscience to resist the impact of environmental collapse, and explores how this trope is incorporated in Bacigalupi’s narrative to celebrate human striving for hope and survival in an imagined environmental future marked by a self-created agro-scientific grotesqueness

FORMULATION, IN VITRO, AND IN VIVO EVALUATION OF TASTE-MASKED ORAL DISINTEGRATING TABLETS OF FEXOFENADINE HYDROCHLORIDE USING SEMISYNTHETIC AND NATURAL SUPERDISINTEGRANTS

Objective: The aim of the present research work was to prepare and evaluate taste-masked oral disintegrating tablets (ODT) of Fexofenadine hydrochloride. Methods: In the present work, Eudragit EPO, a taste masking agent and Karaya gum (GK) (natural), Sodium starch glycolate, and Croscarmellose sodium (CCS) (semi-synthetic) super disintegrants in three ratios (3, 6,9%) were used. Taste masked granules were prepared by different ratios of the drug: Eudragit EPO (1:1, 1:1.5, 1:2) by wet granulation method. The optimized taste-masked granules (1:2) were selected by sensory evaluation test to prepare 9 Fexofenadine ODT (FH1-FH9) formulations. These were evaluated for different parameters. Then desirability function (DF) was calculated for all formulations using disintegration time (DT), time taken for the tablet to release 90% of the drug (t 90%), and % drug dissolved in 10 min (Q10) as significant parameters. Results: The best formulation (FH6) showed the highest DF value due to less DT and 100% in vitro drug release within 15 min. Thus, FH6 formulation containing 9% CCS was selected as the best among the prepared formulations to which in vivo studies were performed on rabbits to find maximum plasma concentration (Cmax), time taken to reach maximum concentration (tmax), area under the curve (AUC), rate of elimination (Kel), absorption rate (Ka) and half-life(t1/2) and compared with Fexofenadine (Allegra) marketed tablets. Total bioavailability was increased for the test formulation compared to the reference formulation. Conclusion: Fexofenadine was successfully prepared as ODT with increased AUC and decreased tmax to which stability studies were conducted which were found to be stable

SYNTHESIS AND EVALUATION OF NOVEL ISATIN DERIVATIVES FOR ANTIMICROBIAL ACTIVITY

Final compound 3-({substituted phenyl [phenyldiazenyl] methylidene} hydrazinylidene)-1,3-dihydro-2H-indol-2-ones (VIa – VIe) which were prepaped by treatment of 3-(benzylidenehydrazinylidene)-1,3-dihydro-2H-indol-2-one(V) with hydrochloric acid, sodium nitrite and aryl amines (like anilines) in good yields. The structures of all compounds were confirmed by IR, 1HNMR and mass spectral analysis. All the compounds were screened for their antibacterial and antifungal activities by using cup – plate method. Out of these compounds VIa and VId exhibited maximum activity. All the text compounds showed mild to moderate antibacterial and antifungal activities.Key words: Isatin Derivatives, 1HNMR, IR, Antibacterial Activity and Antifungal Activity

A NEW PARALLEL INTERFERENCE CANCELLATION ALGORITHM FOR RAKE SYSTEMS

In order to suppres the multi-path interference (MPI) in the DS CDMA system, a new RAKE receiver based on parallel interference elimination is first proposed in this paper data symbol tentative decision is obtained by linear decision; the multi-path interference are evaluated by tentative decision and known user information. Then the performance over Rayleigh fading channel are analyzed and compared to conventional parallel interference cancellation (PIC) and RAKE receiver. It is shown that RAKE receiver performance can be improved greatly by using this method with simple structure and easy implementation