104 research outputs found
Optical Imaging With a High-Resolution Microendoscope to Identify Cholesteatoma of the Middle Ear
Objectives/Hypothesis: High-resolution optical imaging is an imaging modality that allows visualization of structural
changes in epithelial tissue in real time. Our prior studies using contrast-enhanced microendoscopy to image squamous cell
carcinoma in the head and neck demonstrated that the contrast agent, proflavine, has high affinity for keratinized tissue.
Thus, high-resolution microendoscopy with proflavine provides a potential mechanism to identify ectopic keratin production,
such as that associated with cholesteatoma formation, and distinguish between uninvolved mucosa and residual keratin at
the time of surgery.
Study Design: Ex vivo imaging of histopathologically confirmed samples of cholesteatoma and uninvolved middle ear
epithelium.
Methods: Seven separate specimens collected from patients who underwent surgical treatment for cholesteatoma were
imaged ex vivo with the fiberoptic endoscope after surface staining with proflavine. Following imaging, the specimens were
submitted for hematoxylin and eosin staining to allow histopathological correlation.
Results: Cholesteatoma and surrounding middle ear epithelium have distinct imaging characteristics. Keratin-bearing
areas of cholesteatoma lack nuclei and appear as confluent hyperfluorescence, whereas nuclei are easily visualized in specimens
containing normal middle ear epithelium. Hyperfluorescence and loss of cellular detail is the imaging hallmark of keratin,
allowing for discrimination of cholesteatoma from normal middle ear epithelium.
Conclusions: This study demonstrates the feasibility of high-resolution optical imaging to discriminate cholesteatoma
from uninvolved middle ear mucosa based on the unique staining properties of keratin. Use of real-time imaging may facilitate
more complete extirpation of cholesteatoma by identifying areas of residual disease
Enkebrændinger i Indien:119 enker og en glemt indisk konge
Artiklen tager udgangspunkt i nogle få linier fra en brev fra Roland Crappé til Christian 4., hvori han beskriver enkebrændinger og angiver en glemt konge af Thanjavur i det sydlige Indien. I artiklen beskrives fænomenet enkebrændinger (sati), dens religiøse oprindelse i hindu samt den glemte konge
Den Arabiske Rejse og Asiatisk Kompagni 1763-1766:Fire breve og en veksel fra Carsten Niebuhr i Bombay til Guvernør Abbestée i Trankebar
Two Short Presentations on Mass Cleansing of Large Amounts of Mould Infested Files and Conservation of Heavily Damaged Files from the Danish Tropical Colonies in India 1670-1730 - Tranquebar
Den Arabiske Rejse og Asiatisk Kompagni 1763-1766:Fire breve og en veksel fra Carsten Niebuhr i Bombay til Guvernør Abbestée i Trankebar
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