A short G1 phase is an intrinsic determinant of naïve embryonic stem cell pluripotency

AbstractA short G1 phase is a characteristic feature of mouse embryonic stem cells (ESCs). To determine if there is a causal relationship between G1 phase restriction and pluripotency, we made use of the Fluorescence Ubiquitination Cell Cycle Indicator (FUCCI) reporter system to FACS-sort ESCs in the different cell cycle phases. Hence, the G1 phase cells appeared to be more susceptible to differentiation, particularly when ESCs self-renewed in the naïve state of pluripotency. Transitions from ground to naïve, then from naïve to primed states of pluripotency were associated with increased durations of the G1 phase, and cyclin E-mediated alteration of the G1/S transition altered the balance between self-renewal and differentiation. LIF withdrawal resulted in a lengthening of the G1 phase in naïve ESCs, which occurred prior to the appearance of early lineage-specific markers, and could be reversed upon LIF supplementation. We concluded that the short G1 phase observed in murine ESCs was a determinant of naïve pluripotency and was partially under the control of LIF signaling

CRISPR/Cas9: a breakthrough in generating mouse models for endocrinologists

International audienceCRISPR/Cas9 is a recent development in genome editing which is becoming an indispensable element of the genetic toolbox in mice. It provides outstanding possibilities for targeted modification of the genome, and is often extremely efficient. There are currently two main limitations to in ovo genome editing in mice: the first is mosaicism, which is frequent in founder mice. The second is the difficulty to evaluate the advent of off-target mutations, which often imposes to wait for germline transmission to ensure genetic segregation between wanted and unwanted genetic mutations. However rapid progresses are made, suggesting that these difficulties can be overcome in the near future

Isolation of rabbit and goat ES-like cells with human ES cell features

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Regulation of cyclin D1 expression in mouse embryonic stem (ES) cells : the role of the Ras/MAPK and PI3K/Akt pathways

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Isolation of rabbit ES-like cells with human ES cell features

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Isolation of rabbit ES-like cells with human ES cell features

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Obtention de cellules de lapin de type ES dotées de caractéristiques comparables à celles des cellules ES humaines

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Obtention de cellules ES-like de lapin dotées de caractéristiques comparables à cellules des cellules ES humaines

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Virofection: a new procedure to achieve stable expression of genes transferred into early embryos

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