Major HLA class II alleles/allele groups in mothers and their children.

<p>Major HLA class II alleles/allele groups in mothers and their children.</p

PCR and sequencing primers used for genotyping HLA class II genes.

<p>PCR and sequencing primers used for genotyping HLA class II genes.</p

Phenotypes of HIV-infected unstimulated CD4+ T cells.

<p>(A) CD4+ T cells were evaluated for infection by intracellular staining for HIV p24. Cells were gated on singlets, lymphocytes and live cells before discriminating CD4+ T cells. Infected (p24+) and uninfected cells (p24-) were phenotyped for markers of Tregs (defined as CD25^hi, CD127^lo) and T cell activation (CD69, HLA DR). (B) Comparison of T cell phenotypes between infected and uninfected CD4+ T cells (n = 12). Higher frequencies of infected cells were activated (CD69+ HLA DR+, CD69+ HLA DR- or CD69- HLA DR+) or demonstrated a Treg phenotype (CD25^hi CD127^lo) compared to uninfected cells. P-values were calculated using the Wilcoxon signed-rank test for matched pairs.</p

Infection of unstimulated PBMC inoculated with HIV_ML1956.

<p>Graph shows average level of HIV infection of replicate wells as determined by HIV p24 ELISA. Individual patients are represented by their study ID numbers. The number of replicate wells demonstrating productive infection (out of 6) is indicated below the patient study ID numbers.</p