Additional file 1 of RNA sequencing reveals differential long noncoding RNA expression profiles in bacterial and viral meningitis in children

Supplementary Material

miRNA* mediated mRNA cleavage detected by degradome sequencing in maize.

<p>miRNA* mediated mRNA cleavage detected by degradome sequencing in maize.</p

Differential expression of newly identified miRNAs in response to N deficiency in shoots (A) and roots (B).

<p>Only miRNA genes with > 2-fold relative change were shown. Selected miRNAs from shoots and roots were validated by small RNA northern blot (C). A 40 µg small RNA was loaded per lane and hybridized with corresponding ³²P-labeled probes. 5s/tRNA is shown as a loading control.</p

Detection of novel miRNA candidate identified by small RNA library (A) or degradome (B) sequencing.

<p>Plants were grown hydroponically with full-strength Hoagland's nutrient solution for 2 weeks and then transferred to the N-free or N-replete medium for 2 days before small RNA was isolated from leaves and roots. Five micrograms of small RNA from each sample was loaded and hybridized with corresponding ³²P-labeled probes. 5s/tRNA is shown as a loading control.</p

Predicted targets of novel miRNAs identified by the degradome sequencing in maize.

<p>Predicted targets of novel miRNAs identified by the degradome sequencing in maize.</p

Differential expression of conserved miRNAs in response to N deficiency in shoots (A) and roots (B).

<p>Only miRNA genes with > 2-fold relative change are shown. Selected miRNAs from roots were validated by Real time RT-PCR (C) or small RNA northern blot (D). Maize seedlings were grown hydroponically with full-strength Hoagland's nutrient solution for 2 weeks and then transferred to the N-free or N-sufficient medium for 2 d before total/small RNA was isolated. Real-time RT-PCR quantifications were normalized to the expression of <i>ZmGAPC2</i>. The results represent SD of three replicates. A 40 µg small RNA was loaded per lane and hybridized with corresponding ³²P-labeled probes. 5s/tRNA is shown as a loading control.</p

Size distribution of redundant (A) and unique (B) small RNA sequences.

<p>nt, nucleotides.</p

A possible functional network of N-limitation responsive miRNAs in maize seedlings.

<p>− represents negative regulation; + represents positive regulation.</p