Additional file 1 of Systematic optimization and evaluation of culture conditions for the construction of circulating tumor cell clusters using breast cancer cell lines

Additional file 1: Figure S1. The molecular characteristics of CTC clusters in MDA-MB-231 and 4T1 cells. (A) Representative images of the appearance of 4T1 cell clusters and CTC clusters from patients. F-actin (green), DAPI (nuclei, blue). B TEM images of 4T1 cell clusters. J, cell-cell junction. The red arrow points to the tight junction. C Western blot analysis of the protein levels of plakoglobin in MDA-MB-231 and 4T1 adherent cells and cell clusters. Immunofluorescence analysis of the protein levels difference of plakoglobin (D) and ZO-1 (E) in MDA-MB-231 and 4T1 adherent cells and cell clusters. DAPI (nuclei, blue), plakoglobin (red), ZO-1 (red)