2 research outputs found
Harnessing Cell Dynamic Responses on Magnetoelectric Nanocomposite Films to Promote Osteogenic Differentiation
The
binding of cell integrins to proteins adsorbed on the material
surface is a highly dynamic process critical for guiding cellular
responses. However, temporal dynamic regulation of adsorbed proteins
to meet the spatial conformation requirement of integrins for a certain
cellular response remains a great challenge. Here, an active CoFe<sub>2</sub>O<sub>4</sub>/polyÂ(vinylidene fluoride-trifluoroethylene)
nanocomposite film, which was demonstrated to be an obvious surface
potential variation (Δ<i>V</i> ≈ 93 mV) in
response to the applied magnetic field intensity (0–3000 Oe),
was designed to harness the dynamic binding of integrin-adsorbed proteins
by in situ controlling of the conformation of adsorbed proteins. Experimental
investigation and molecular dynamics simulation confirmed the surface
potential-induced conformational change in the adsorbed proteins.
Cells cultured on nanocomposite films indicated that cellular responses
in different time periods (adhesion, proliferation, and differentiation)
required distinct magnetic field intensity, and synthetically programming
the preferred magnetic field intensity of each time period could further
enhance the osteogenic differentiation through the FAK/ERK signaling
pathway. This work therefore provides a distinct concept that dynamically
controllable modulation of the material surface property fitting the
binding requirement of different cell time periods would be more conducive
to achieving the desired osteogenic differentiation
Controlled Release of Naringin in Metal-Organic Framework-Loaded Mineralized Collagen Coating to Simultaneously Enhance Osseointegration and Antibacterial Activity
Two
important goals in orthopedic implant research are to promote osseointegration
and prevent infection. However, much previous effort has been focused
on the design of coatings to either enhance osseointegration while
ignoring antibacterial activity or vice versa, to prevent infection
while ignoring bone integration. Here, we designed a multifunctional
mineralized collagen coating on titanium with the aid of metal-organic
framework (MOF) nanocrystals to control the release of naringin, a
Chinese herbal medicine that could promote osseointegration and prevent
bacterial infection. The attachment, proliferation, osteogenic differentiation,
and mineralization of mesenchymal stem cells on the coating were significantly
enhanced. Meanwhile, the antibacterial abilities against Staphylococcus aureus were also promoted. Furthermore,
release kinetics analysis indicated that the synergistic effect of
a primary burst release stage and secondary slow release stage played
a critical role in the performance and could be controlled by the
relative concentrations of MOF and naringin. This work thus provides
a novel strategy to engineer multifunctional orthopedic coatings that
can enhance osseointegration and simultaneously inhibit microbial
cell growth