Identifikasi Escherichia coli pada Tangan Penjamah Makanan di Kantin Kampus FK Ukrida Tahun 2016

Kontaminasi E. coli pada makanan cukup tinggi di Indonesia terutama di Jakarta. Tingkat kontaminasi oleh E. coli adalah 65,5% . Kejadian Luar Biasa (KLB) keracunan makanan juga masih tinggi yaitu 31.919 kasus tahun 1997 dengan angka kematian kasus 0,15%. Penelitian ini bertujuan untuk mengidentifikasi adanya bakteri Escherichia coli pada tangan penjamah makanan di kantin Kampus Fakultas Kedokteran Universitas Krida Wacana (FK Ukrida) dengan metode total purposive sampling sebanyak 17 sampel yang diteliti secara biokimia, menggunakan Analytical Profile Index Test. Pengambilan sampel dengan swab tangan hanya dilakukan satu kali saja untuk setiap penjamah makanan, menggunakan kapas lidi steril yang sudah dicelupkan ke dalam larutan NaCl 0,9% pada seluruh permukaan tangan dan sela-sela jarinya. Hasil swab tangan dibiakkan pada medium agar Eosin Methylen Blue. Hasil penelitian yang telah dilakukan menunjukkan bahwa bakteri yang terdapat pada tangan penjamah makanan di kantin kampus FK Ukrida adalah koloni jamur dan bakteri gram positif, Pantoea spp, Raoultella planticola.Kata kunci: Escherichia coli, penjamah makanan, kantin kampus FK Ukrid

The Relationship Between Stress Level and Sleep Quality of Nurses in Jakarta During the COVID-19 Pandemic

Coronavirus disease (Covid-19) is an infectious disease known as a severe acute respiratory syndrome. The first case of Covid-19 was identified in Wuhan, China on December 31, 2019. Health workers around the world are at the forefront of handling the COVID-19 pandemic. One of the health workers who is very important in handling COVID-19 cases is a nurse. The considerable increase of Covid-19 cases makes nurses have a heavier workload, struggle, and reduced rest periods. Heavy workloads and reduced rest periods can generally increase fatigue and affect sleep quality and stress levels which negatively impact work balance. This study's objective was to determine whether there was a relationship between the stress level and the quality of sleep of nurses in Jakarta during the Covid-19 pandemic. This study used 75 samples of nurses who were actively working during the Covid-19 pandemic in Jakarta, female, and aged 20-40 years. The sampling technique was snowball sampling. Data collection was through a questionnaire distributed via a Google form. The questionnaire included questions about the characteristics of the respondents, the Perceived Stress Scale (PSS-10), and the Pittsburgh Sleep Quality Index (PSQI). The study showed that there was a relationship between stress levels and the sleep quality of nurses in Jakarta during the Covid-19 pandemic