Pemanfaatan Casa Dalam Observasi Motilitas Spermatozoa Semen Cair Sapi Madura Dalam Pengencer Berbeda

The purpose of this study was to measure the motility of Madura bullspermatozoa using three different diluents (tris aminomethane, CEP-2 andskim milk) duringcold storage. The research material used were 2 Madurabulls. Collecting semen method used artificial vagina, followed by freshsemen analysis and processing of liquid semen. Observation of liquid semenquality was carried out up to the 5th days of cold storage. Motilityexamination of semen liquid used Computerized Assisted Semen Analysis(CASA), namely SCA v.5.2. The parameters measured were: progressive motility,motility, velocity straight linear (VSL), velocity curve linear (VCL),velocity average pathway (VAP), straightness (STR), linearity (LIN), wobble(WOB), hyperactivity (H), Beat cross frequency (BCF), amplitude lateral head(ALH). Data was analyzed by Minitab 17. Motility and progressive motility ofspermatozoa in tris aminomethane and CEP-2 were higher than skim milk for 5days of cold storage. The VCL value of tris aminomethane was higher thanCEP-2 and skim milk. VSL scores barely show the difference among diluents.VAP values in tris aminomethaneand CEP-2 are higher than skim milk at thebeginning of storage. The LIN, STR and WOB values of the CEP-2 and skim milkyield higher values than tris aminomethane. The percentage value ofhyperactivity spermatozoa was same among diluents. The ALH value oftrisaminomethane was higher than CEP-2 and skim milk. BCF values in CEP-2was higher than skim milk and tris aminomethane. Tris aminomethane and CEP-2can support the motility of Madura bull spermatozoa than skim milkfor 5 daysof storag

Fertilitas Spermatozoa Ayam Buras Dengan Penambahan Antioksidan Glutathione Dalam Pengencer Ringer\u27s Selama Simpan Dingin

Testing of spermatozoa fertility in vivo is needed to find out the extent of the abilityof spermatozoa fertilization in the female reproductive tract. The fertility ability ofspermatozoa in fertilizing the ovum is indicated by the number of fertile eggs fromthe number of eggs incubated resulting from the mating with Artificial Insemination(AI). The aim of this research is to know the percentage of fertility of eggs from AIusing semen which has been stored cold for 8 hours on Ringe\u27s diluent with theaddition of antioxidant glutathione. Collection semen with teaser female method isdone in Poultry Breeder Installation of Sekolah Tinggi Penyuluhan Pertanian (STPP)Malang. The 30 female chickens were divided into 3 groups of treatment P0: AIusing fresh semen with Ringer\u27s diluent, P1: AI using cold cement with Ringer\u27sDiluent (8 hours duration storage) and P2: AI using cold semen with 0.5 mMaddition in Diluent Ringe\u27s (8 hours duration storage). Experiment design usingComplete Randomized Design (RAL) and nonparametric data analysis with chi-square.The observed variable was egg fertility calculated based on egg fertility percentage.The results showed that the dilution treatment had a significant effect (P <0,05) onfertility egg, with the best result indicated by P0 that is 76% fertility, followedby P2 with fertility 42% and P1 which showed the lowest fertility was 34%. IB withcold cement has not demonstrated a good percentage of fertility despite 0.05 mMglutathione added as an antioxidant. It is recommended that AI in domestic poultryusing fresh semen with Ringe\u27s diluent, is done as soon as possible to obtain optimumfertility

Keberhasilan IB Menggunakan Semen Beku Hasil Sexing Dengan Metode Sedimentasi Putih Telur Pada Sapi PO Cross

The study was conducted in subdistrict Pakis, Malang Regency from May to July 2013. The study materials were 54 Ongole crossbred cattle. Twenty seven cattle were inseminated using non sexed semen (P0) and 27 cattle were inseminated using sexed semen (P1). Samples were selected randomly using criterion that semen deposition was in position 4+. Gestation evaluation was calculated based on non return rate (NRR), service per conception (S/C) and conception rate (CR). The study showed that P0 had 74% of NRR and P1 had 59% of NRR. S/C was found 1.37 at P0 and 1.48 at P1. CR was found 74% at P0 and 59% at P1. The study concluded that non sexed semen increased better gestation for Ongole crossbred cattle than sexed semen The study suggested that sexed semen remains able to be inseminated to those cattle with high attention on the sexing procedures

Tampilan Reproduksi Sapi Perah Pada Berbagai Paritas Di Wilayah KUD Batu

The purpose of this study was to determine the performance of reproduction in dairy cattle, the views of service per conception, days open, and calving interval. The material used in this study is the implementation of recording data or artificial insemination in 100 dairy cows females have at least twice to parturition known calving interval. Samples were selected randomly within the same district in Batu. The method used in this research is a case study. The data is taken primary and secondary data. Primary data retrieval is done by direct observation (observation), which includes several variables, where the variables include: the identity of the breeder, the number of cattle, cages and feeding and drinking. As for the secondary data obtained by recording the card Artificial Insemination (AI) owned by farmers. Sampling was done by purposive sampling and random with the following conditions: sampling was done by purposive sampling with the following conditions: minimal yearling heifer parturition twice and had a complete recording AI, including, the date AI and the date of parturition. Results from this study is the performance of reproduction in the dairy cooperatives Batu showed unfavorable results, average - the average total value of S/C of parity 1 s / d 6 is, 1.93 ± 1.18. As for the average - the average total value of DO and CI parity 1 s / d 5 is, 171.70 ± 132.20 days and 450.92 ± 132.83 days. In addition, the assessment of S / C, DO and CI at different parity using statistical tests showed that the S / C, DO and CI at various parity was not significantly different (P> 0.05).It concluded that reproduction display AI results in the KUD BATU less than the maximum and the difference between parity does not affect the value of the S / C, DO, and CI

Kualitas Semen Dan Produksi Semen Beku Pada Bangsa Sapi Dan Bulan Penampungan Yang Berbeda

The purpose of this research was to determine semen quality and frozen semen production of beef cattle at different month collection in Lembang Artificial Insemination Center. Beef cattle strain that used for this research are Aceh, Angus, Brahman, Limousine, Madura, Ongole and Simmental. The research parameter included semen volume, pH, motility of spermatozoa, concentration of spermatozoa, number of spermatozoa, number of spermatozoa motil and number of frozen semen straw. The method of this research using factorial completely Randomized Design and each had 3 replication per treatment. The different of beef cattle have given a significant effect of semen volume (P<0.01), mortility of spermatozoa (P<0.01), concentration of spermatozoa (P<0.01), number of spermatozoa (P<0.01), number of spermatozoa motil (P<0.01) and pH (P>0.05). Different month collection have a significant influence of semen volume (P<0.01), pH (P<0.05), motility of spermatozoa (P<0.01), concentration of spermatozoa (P<0.01), number of spermatozoa (P<0.01), number of spermatozoa motil (P<0.01). Interaction between breed of beef cattle and different month of collection have given a significant influence of semen volume (P<0.01), motility spermatozoa (P<0.01), concentration spermatozoa (P<0.05) and number of spermatozoa motil (P<0.01)

Penampilan Reproduksi Sapi Peranakan Ongole (PO) Dan Sapi Peranakan Limousin Di Kecamatan Sawoo Kabupaten Ponorogo Dan Kecamatan Tugu Kabupaten Trenggalek

The purpose of this study was to evaluate artificial insemination program on the reproduction performance of Ongole crossbred and Limousin crossbred cattle. The materials of the study were Ongole crossbred and Limousin crossbred cattle. Descriptive analysis was used to determine service per conception (S/C), days open (DO), calving interval (CI), conception rate (CR) and calving rate (CvR). Meanwhile, t-test was used to analyse differences among those variables. The study showed that the value of S/C, DO and CI between Ongole crossbred and Limousin crossbreed cattle differed significantly (P<0.05). The average S/C, DO, CI of Ongole crossbred cattle were 1.3±0.32; 156.9±29.33 days; 430±43.72 days respectively. In addition, Ongole crossbred cattle had calving rate (CvR) as well as conception rate (CR) was 74%. Meanwhile Limousin crossbed cattle had 1.5±0.39 of S/C; 172.9±19.21 days of DO; 451.3±19.61 days of CI. Moreover, calving rate (CvR) and conception rate (CR) of Limousin crossbed cattle was 52%. The study concluded that reproduction performances of Ongole crossbred cattle were better than that of Limousin crossbred cattle

Pengaruh Penggantian Bovine Serum Albumin (BSA) Dengan Putih Telur Pada Pengencer CEP-2 Terhadap Kualitas Semen Sapi Peranakan Ongole Pada Suhu Penyimpanan 3-5oC

Purpose of this research was to investigate the effect of Bovine Serum Albumin substitution by albumen on CEP-2 to semen quality Ongole CrossBred bull stored at 3-5oC. Research was conducted at Research Centre Beef Cattle Laboratory, Grati, Pasuruan on February 2016. Semen diluent was divided into two groups, there were P0 (90% CEP-2 + 10% Egg Yolk (EY)); and P1 (90% CEP + 0.4% albumen + 10% EY). Data of the research were analyzed using paired design t test. The result showed that after eight days chilled preservation, percentage of motility P1 (47.4±10.9%) was higher than P0 (47±9.2%). Percentage of viability P0 (83.1±1.9%) was higher than P1 (81.3±1.5%). Percentage of abnormality P1 (3.6±0.4%) was lower than P0 (3.8±0.3%). Total motile sperm count after six days chilled preservation was significantly higher in all treatments compared to the standard criteria of SNI 40% motile sperm/ml. The conclusion of this research was 0,4% albumen could replace the BSA capability on CEP-2