Sequences of sorted fetal and non-fetal NKT cell populations.

<p>Sequences of sorted fetal and non-fetal NKT cell populations.</p

Percentages of IL-4 and IFN-γ secreting NKT cells within total, CD4⁺ and DN subsets.

<p>Horizontal bars indicate medians of percentages of IFN-γ (upper panel) and IL-4 (lower panel) secreting NKT cells within total (left), DN (middle) and CD4⁺ (right) NKT cell subsets. Cord blood (CB) NKT cells and peripheral blood (PB) NKT cells from adults are compared.</p

Representative dot-plots of IL-4 and IFN-γ secreting NKT cells within total, CD4⁺ and DN subsets.

<p>NKT cells from adult peripheral blood (upper two panels) and cord blood NKT cells (lower two panels) are compared. NKT cells were first identified by double positive staining of Vα24 and Vβ11 TCR chains (left). IFN-γ and IL-4 secreting NKT cells are shown within total (left), DN (middle) and CD4⁺ (right) NKT cell subsets.</p

Percentages of IL-4 and IFN-<i>γ</i> secreting NKT cells within NKT subsets.

a<p>Data are shown as medians with (min-max.).</p

Percentages of chemokine receptor expressing Vα24⁺Vβ11⁺ NKT cells.

a<p>Data are shown as medians with (min-max.).</p

The Circos plot represents significantly enriched pathways associated with regulated genes in the placentae of obese women <i>vs</i>. normal weight women, detected using the Ingenuity Pathway Analysis library of canonical pathways.

<p>Outside the circle dysregulated genes and IPA pathways together with biological process are indicated. All genes are down-regulated (highlighted in green), except for HLA-DRB1 and CCL2 genes that are up-regulated (highlighted in red).</p

Shows the two highest scoring networks in IPA derived from the analysis of dysregulated genes in placentas of obese women <i>vs</i>. normal weight women.

<p>(<b>A)</b> Genes involved in embryonic development, organismal development and cancer. (<b>B)</b> Dysregulated genes involved in cellular movement, haematological system development and function, and immune cell trafficking. The intensity of the node colour indicates the degree of up- (red) or down- (green) regulation of gene expression. A white node represents a gene that is not part of our dataset, but is incorporated into the network through relationships with other genes. Nodes are displayed using various shapes that represent the functional class of the gene product, and a biological relationship between two nodes is represented as a line. Detailed information about the figure symbols can be found at <a href="http://www.ingenuity.com" target="_blank">www.ingenuity.com</a>.</p

List of dysregulated genes in term placentas from obese women <i>vs</i>. normal weight women.

<p>The genes identified in previous transcriptome studies in human placentas in health and disease are indicated.</p

Microarray validation by real-time PCR.

<p>The expression levels of genes <i>AREG</i>, <i>CCL2</i>, <i>FSTL3</i>, <i>IGFBP1</i> and <i>MMP12</i> from our gene array analysis in comparison with real-time PCR gene expression levels are presented. QRT-PCR data are shown as dCp values (Cp_targetgene-Cp_{referencegene}), where higher dCp values represent lower expression and therefore, graphs are represented with reversed Y-axis. Mann-Whitney <i>U</i>-test *p <0.05, n = 5 samples/group.</p

Clinical characteristics of the participating women in the study and their perinatal outcomes.

<p>Clinical characteristics of the participating women in the study and their perinatal outcomes.</p