BRIG analysis: Location of genomic regions unique to ST25 case isolates.

<p>The 3 phage sequences linked to <i>Kpp</i> ST25 isolates are highlighted with red bars. C, case; CR, case-related; cnda, contemporary non disease associated; H, historical.</p

Clinical interventions and efficacy following the diagnosis of piglet septicaemia due to <i>Kpp</i>.

<p>Clinical interventions and efficacy following the diagnosis of piglet septicaemia due to <i>Kpp</i>.</p

Frequency of gross lesions in thirty five piglets which died of septicaemia due to <i>Klebsiella pneumoniae</i>.

<p>Frequency of gross lesions in thirty five piglets which died of septicaemia due to <i>Klebsiella pneumoniae</i>.</p

Comparison of virulence determinants in ST25 <i>Klebsiella pneumoniae</i> isolates to other <i>K</i>. <i>pneumoniae</i> from porcine cases.

<p>Comparison of virulence determinants in ST25 <i>Klebsiella pneumoniae</i> isolates to other <i>K</i>. <i>pneumoniae</i> from porcine cases.</p

Clinical details for English pig herds with outbreaks of septicaemia due to <i>Kpp</i> infection.

<p>Clinical details for English pig herds with outbreaks of septicaemia due to <i>Kpp</i> infection.</p

Emergence of <i>Klebsiella pneumoniae</i> subspecies <i>pneumoniae</i> as a cause of septicaemia in pigs in England - Fig 4

<p><b>(A) Gel picture showing electrophoretic separation of plasmids carried by 16 <i>Kpp</i> isolates</b>, migrating in 0.8% agarose (TBE), for 270 min at 150 v, 4°C, are shown. Yellow stars indicate location of pKPMC25 common to <i>Kpp</i> ST25 isolates. The plasmids in lane 1 and 21 are supercoiled DNA ladder. Lane 2, 12, 20 shows the reference plasmid bands from strain 39R861 (148 kb, 63 kb, 36 kb, genomic DNA band and 6.8 kb. Lane 3, 8–10 and 16–18 are <i>Kpp</i> ST25 isolates. Lanes 4–7, 11, 13–15 and 19 are non-ST25 <i>Kpp</i> isolates. (<b>B). Overview of pKPMC25 plasmid sequence features and comparison to closest matching plasmids</b>.</p

Results of <i>Kpp</i> multilocus sequence typing for British historical (1993–2010) isolates, porcine contemporary non-disease associated (CNDA) isolates collected 2011 to 2014 and for outbreak cases (C) and case-related but non cases (CR).

<p>Results of <i>Kpp</i> multilocus sequence typing for British historical (1993–2010) isolates, porcine contemporary non-disease associated (CNDA) isolates collected 2011 to 2014 and for outbreak cases (C) and case-related but non cases (CR).</p

Phylogenetic SNP tree analysis of <i>Klebsiella</i> genomes, using <i>Klebsiella pneumoniae</i> NTUH-K2044 as reference genome.

<p>Shaded isolates the ST25 case isolates. There were a total of 195803 SNP positions in the final dataset. Node colours indicate bootstrap support according to figure legend (Blue = low bootstrap support, Red = high bootstrap support).</p

Virulence gene analysis of 51 isolates from 1993 to 2011.

<p>Virulence gene analysis of 51 isolates from 1993 to 2011.</p