Expression profile of miR-200f and EMT-transcriptional inducers in breast tumors.

<p>The expression levels of miR-200 family members (<b>A</b>) and EMT-transcriptional inducers (<b>B</b>) were quantified by qRT-PCR in 70 breast cancer samples. Data are depicted as box-and-whisker plots. Adjusted <i>p</i>-values are shown where significant differences were found (Wilcoxon rank-sum test). ER+ (estrogen receptor positive tumors), HER2+ (HER2-positive tumors), TN (triple negative; ER−, PR−, HER2−), MBC (metaplastic breast carcinomas). (<b>C</b>) Upper heatmap represents Pearson coefficient (R) correlation values between the expression of EMT-transcriptional inducers and miR200f members. Bottom heatmap depicts the level of statistical significance (P) of the correlations.</p

miR-200f and EMT-transcriptional inducers are modulated during <i>in vitro</i> EMT in breast basal cell lines.

<p>Expression of (<b>A</b>) miR-200f and (<b>B</b>) EMT-transcriptional inducers was evaluated by qRT-PCR in the sorted epithelial (EpCAM+) and mesenchymal (Fibros) subpopulations within MCF12A and Myo1089 cell lines. Data are normalized to the expression of <i>RNU48</i> and <i>18S</i> respectively. Bars represent mean expression changes ±SE in Fibros subpopulation relative to EpCAM+ cells (baseline). Three biological replicates were measured. Moderated t-test was performed to evaluate statistical significance (<i>p</i><0.001 for all miRNAs analyzed in panel A. <i>NS</i>, non significant). (<b>C</b>) Methylation status of <i>miR-200f</i> promoter sequences in EpCAM+ and Fibros subpopulations within MCF12A and Myo1089 cell lines. Histogram bars represent averaged methylation levels (±SE) for the promoter sequences of <i>miR-200f loci</i>. Statistically significant differences are indicated (*<i>p</i><0.001, Student’s t-test).</p