Relationships among the <i>N</i><i>. caninum</i> MLGs (n = 82) estimated by eBURST analysis.

<p>Each complete MLG is represented by a dot and the genotype number assigned in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072678#pone.0072678.s003" target="_blank">Table S1</a> (Numbers 1-11 represent worldwide MLGs; 12-64 Spanish MLGs; 65-80 Argentinean MLGs; 81-88 Scottish MLGs; and 89-95 German MLGs). MLG dots were also coloured according to their geographical origin (see legend). The dot diameter is proportional to the number of samples with identical MLG (see legend). Single locus variants (SLV) are connected by black lines and double loci variants (DLV) by blue lines. MLGs clusters (n=9) are represented. Main MLGs clusters (n=3) are showed by clear circles. Number and name in the squares identifies the herd origin and worldwide isolates, respectively (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0072678#pone.0072678.s003" target="_blank">Table S1</a>). Note that singletons were excluded from the snapshot representation (worldwide n=7; Spanish n=7; Argentinean n=2; Scottish n=3; and German n=2).</p

Clustering of <i>N</i><i>. caninum</i> country populations based on Principal Component Analysis (PCA) and Neighbor Joining (NJ).

<p>(A) Graphics represent the genetic relationships between the MLGs from each country population based on a covariance matrix. Colours indicate geographic origin (see legend). The proportion of variation in the dataset for each axis is indicated in parentheses. (B) Unrooted NJ tree inferred from allele shared genetic distances. Each tip represents a single MLG. Woldwide MLGs are identified by isolate name. Colour of circles in terminal branches indicates geographic origin (see legend). Percentage bootstrap values were generated from 1,000 replicates. Bootstrap values ≥70% are shown in black circles. Scale bar represents branch lengths. NJ tree based in Cavalli-Sforza distances depicted similar topologies (data not shown).</p