5 research outputs found
Biological characterization of a beta-galactosidase expressing clone of Trypanosoma cruzi CL strain
Biological characterization of a β-galactosidase expressing clone of Trypanosoma cruzi CL strain
Clone CL B5 of Trypanosoma cruzi is a β-galactosidase
expressing organism that was genetically transfected to be used for in
vitro pharmacological screening. Biological parameters were determined,
evaluating growth kinetics of epimastigotes, metacyclogenesis,
infectivity to mammalian cell lines, parasitemia kinetics in mice and
sensibility to nifurtimox and benznidazole. Differences in relation to
other strains and CL parental strain were found, the most important
being the incapability to produce death to mice in spite of the high
inoculum used. However, it possesses the required features to be used
for in vitro drug screening. Data obtained demonstrate that
heterogeneity of T. cruzi appears even among clones of the same strain,
and that these differences found do not prevent the use of clone CL B5
for the purpose that was engineered
In vitro and in vivo anti- Trypanosoma cruzi activity of a novel nitro-derivative
Nitroarylidenemalononitriles and their cyanoacetamide derivatives with
remarkable anti-epimastigote properties, were synthesized attempting to
obtain new 3,5-diamino-4-(5'-nitroarylidene)-4H-thiadiazine 1,1-dioxide
derivatives, which in previous reports had shown anti- Trypanosoma
cruzi activity. Tests to evaluate the cytotoxicity of
compounds were performed on J774 macrophages.
5-nitro-2-thienyl-malononitrile (5NO 2 TM), was the only product which
maintained a high anti-epimastigote activity at concentrations in which
it was no longer cytotoxic, thus it was assayed against intracellular
amastigotes. Its anti-amastigote activity was similar to that of
nifurtimox. Afterwards in vivo toxicity and anti-chagasic activity were
determined. A reduction in parasitemia was observed