17 research outputs found
Fundamental Studies of Liquid Chromatography at the Critical Condition Using Enhanced-Fluidity Liquids
Liquid Chromatography at the Critical Condition Using Enhanced-Fluidity Liquid Mobile Phases
Comparison of Reversed-Phase HPLC Separation Using Carbon Dioxide and Fluoroform for Enhanced-Fluidity Liquid Mobile Phases
Homogeneous Edge-Plane Carbon as Stationary Phase for Reversed-Phase Liquid Chromatography
Carbon stationary phases have been
widely used in HPLC due to their
unique selectivity and high stability. Amorphous carbon as a stationary
phase has at least two sites of interaction with analytes: basal-plane
and edge-plane carbon sites. The polarity and adsorptivity of the
two sites are different. In this work, the edge-plane carbon stationary
phase is prepared by surface-directed liquid crystal assembly. Specific
precursor polymers form discotic liquid crystal phases during the
pyrolysis process. By using silica as the substrate to align the discotic
liquid crystal, edge-plane carbon surfaces were formed. Similar efficiencies
as observed for Hypercarb were observed in chromatograms. The column
efficiency was studied as a function of linear flow rate. A minimum
reduced plate height of 6 was observed in these studies. To evaluate
the performance of the homogeneous edge-plane carbon stationary phase,
linear solvation energy relationships were used to compare these ordered
carbon surfaces to commercially available carbon stationary phases,
including Hypercarb. Reversed-phase separations of nucleosides, nucleotides,
and amino acids and derivatives were demonstrated using the ordered
carbon surfaces, respectively. The column batch-to-batch reproducibility
was also evaluated. The retention times for the analytes were reproducible
within 1–6% depending on the analyte
Planar Electrochromatography Using an Electrospun Polymer Nanofiber Layer
Electrospun polymer nanofiber stationary
phases were examined for
their application to planar electroÂchromatography (PEC). Separations
were performed on polyacryloÂnitrile nanofiber ultra-thin-layer
chromatography (UTLC) plates in 1–2 min using a ternary mobile
phase. The influences of buffer concentration and pH, ratio of organic
modifier, and development time on analyte migration distances were
studied. Band broadening in this system was studied as a function
of distance. The plate height initially decreased and then plateaued
with a minimum plate height value as low as 11 μm. Nanofiber
alignment considerably increased analyte migration rate, but larger
spot sizes were noted when nearly complete fiber alignment was used.
The easily tunable stationary phase thickness can be tailored to a
given separation, where thinner layers promote faster separations
and thicker layers are ideal for more complex mixtures. Compared to
UTLC, PEC offers unique selectivity and decreased analysis time (>4
times faster over 15 mm). Results for a two-dimensional separation
using UTLC and PEC are also reported. These rapid separations required
11 min using a 40 × 40 mm plate and exhibited a significant increase
in separation number (70–77)