7 research outputs found
Characterization of circulating RSV strains among subjects in the OUTSMART-RSV surveillance program during the 2016-17 winter viral season in the United States
<div><p>Background</p><p>Respiratory syncytial virus (RSV) is an established cause of serious lower respiratory disease in infants, elderly and high-risk populations. The OUTSMART surveillance program aims to characterize patient populations and currently circulating RSV strains, and monitor temporal and geographic evolution of RSV F and G proteins in the U.S.</p><p>Methods</p><p>The OUTSMART 2016–17 study collected RSV-positive samples from 25 RSVAlert<sup>®</sup> laboratories from 4 U.S. regions and Puerto Rico during November 2016 through March 2017. Frequencies of A and B subtypes and genotypes were determined for several demographic and geographic variables. To gauge the representativeness of the OUTSMART patients, results were compared to discharge data from the NEDS and NIS databases.</p><p>Results</p><p>A total of 1,041 RSV-positive samples with associated demographic data were obtained and the RSV F gene and second variable region of the G gene were sequenced. The majority of samples (76.0%) came from children under 2 years old: <1 year (48.4%), 1–2 years (27.6%). The OUTSMART patient sample was similar to NEDS and NIS for age, gender, and geographic location. Both OUTSMART and national RSV cases peaked in January. Of OUTSMART samples, 45.3% were subtype A, 53.7% were subtype B and 1.0% were mixed A and B. The percentage of RSV B cases increased with increasing age. Hospitalization (length of hospital stay, LOS, >24 hrs) occurred in 29.0% of patients of which 52.0% had RSV B. Outpatients (LOS <24 hrs) were 64.4% of total of which 73.3% were diagnosed in the ER and discharged, while only 6% were diagnosed in other outpatient settings.</p><p>Conclusions</p><p>The OUTSMART 2016–17 study was representative of the U.S. RSV experience. Geographic and temporal information from the RSV surveillance program will be used to establish a molecular baseline of RSV F and G sequence variability and to help inform development of novel agents for RSV prophylaxis and treatment.</p></div
OUTSMART 2016–17 RSV-positive tests by LOS, referring department and subtype.
<p>OUTSMART 2016–17 RSV-positive tests by LOS, referring department and subtype.</p
OUTSMART 2016–17 RSV-positive tests by LOS, age and subtype.
<p>OUTSMART 2016–17 RSV-positive tests by LOS, age and subtype.</p
Map of participating OUTSMART laboratories during the 2016–2017 season.
<p>Pie-charts represent proportions of RSV A (blue), RSV B (orange), RSV A+B (red) and QNS (yellow) samples per lab. Numbers within the pie charts represent the total number of samples per lab.</p
Temporal distributions of RSV positive tests.
<p>(A) OUTSMART 2016–17 RSV positive tests by RSV subtype. (B) All RSV positive tests in OUTSMART—participating laboratories, and RSV in NEDS and NIS.</p
Comparison of OUTSMART November 2016—March 2017 RSV positive tests with RSV in NEDS and NIS November 2013-March 2014 by age, gender and region.
<p>Comparison of OUTSMART November 2016—March 2017 RSV positive tests with RSV in NEDS and NIS November 2013-March 2014 by age, gender and region.</p
OUTSMART November 2016—March 2017 RSV positive inpatient and Emergency Room cases compared with NIS and NEDS RSV positive cases during November 2013-March 2014 by age group.
<p>OUTSMART November 2016—March 2017 RSV positive inpatient and Emergency Room cases compared with NIS and NEDS RSV positive cases during November 2013-March 2014 by age group.</p