Schematics of the walk-through study using TRCN#40273.

<p>Hairpin cleavage variants identified for the entire length of the oligonucleotide with a step size of 1</p

Walk-through experiment results for 78 ATS siRNA duplexes.

<p>Results of walk-through experiments measured at day 6 post transfection with synthetic siRNA duplexes using EGFPB reporter cell line. (<b>A</b>) Clustered heatmap to show % gain in EGFP signal conferred by 78 ATS siRNA duplexes, tested as singles. (<b>B</b>) Clustered heatmap to show % gain in EGFP signal conferred by siRNA duplexes segregated into three pools that of duplexes active as singles, inactive as singles or with all inclusive. Rep stands for replicate, AVG stands for average of the four replicates.</p

Schematics for Alternate Targeting Sequence Generator (ATSG).

<p>Hairpin inside the cell gets cleaved at its theoretical site and silences its target specifically. Inefficiencies in cleavage would lead to ATSG, generating random targeting sequencing which silence alternate targets, making it extremely difficult to comprehend the eventual phenotypic outcomes.</p