Pathways significantly represented in all oocytes and blastocysts.

<p>Listed entries represent transcripts and their respective NCBI accession numbers that were significantly expressed (P<0.05; q≤0.22) in the appropriate sample(s). “-“ denotes no significantly expressed components were detected in the pathway.</p

Heatmap cluster analysis of individual oocyte and blastocyst gene expression profiles.

<p>Hierarchical clustering was used to compare the gene expression profiles of individual oocytes and blastocysts, with highly expressed genes shown in red, weakly expressed in green. Oocytes show clearly distinct profiles from blastocysts, with oocytes 1 and 2 more similar to each other than to oocyte 3, and blastocyst 1 and 2 more similar to each other than to blastocyst 3. 4-cell embryos were omitted from this analysis based on the low abundance of expressed transcripts.</p

Pathways significantly represented in oocyte and blastocyst samples with similar molecular signatures.

<p>Listed entries represent transcripts and their respective NCBI accession numbers common to oocyte 1 and 2 relative to oocyte 3 and blastocyst 1 and 2 relative to blastocyst 3. “−” denotes no significantly expressed components were detected in the pathway.</p

Scattergraph plots comparing gene expression of individual human oocytes, 4-cell embryos and blastocysts.

<p>Each of three individual samples at each stage is compared to each of the others. Red dots represent transcripts called Present in both samples, yellow dots represent transcripts absent in both samples and blue dots represent transcripts Present in one sample and not the other. The innermost oblique lines represent 2-fold differentially expressed transcripts. Additional pairs of lines represent transcripts expressed at 5, 10- and 20-fold, respectively. As expected, fewer genes were called Present at the 4-cell stage which reflects the degradation of polyA containing maternal transcripts by the 4-cell stage during EGA.</p