NH₃ as a Strong H‑Bond Donor in Singly- and Doubly-Bridged Ammonia Solvent Clusters: 2‑Pyridone·(NH₃)_<i>n</i>, <i>n</i> = 1–3

Mass- and isomer-selected infrared spectra of 2-pyridone·(NH₃)_<i>n</i> clusters with <i>n</i> = 1–3 were measured in the NH and CH stretch fundamental region (2400–3700 cm^–1) using infrared (IR) laser depletion spectroscopy combined with resonant two-photon ionization UV laser detection. The IR depletion spectra reveal three different H-bonding topologies of these clusters: The <i>n</i> = 1 and 2 clusters form ammonia bridges stretching from the N–H to the CO group of the <i>cis</i>-amide function of 2-pyridone (2PY), giving rise to intense and strongly red-shifted (2PY)­NH and ammonia NH stretch bands. For <i>n</i> = 3, two isomers (3X and 3Y) are observed in the IR spectra: The spectrum of 3X is compatible with an ammonia-bridge structure like <i>n</i> = 2, with the third NH₃ accepting an H-bond from C⁶–H of 2PY. The IR spectrum of 3Y exhibits a broad IR band in the 2500–3000 cm^–1 range and is characteristic of a bifurcated double-bridged structure in which the first NH3 accepts an H-bond from the (2PY)­NH and donates two H-bonds to the other two ammonias, both of which donate to the CO group of 2PY. This double-donor/double-bridge H-bonding pattern increases the acceptor strength of the first ammonia and dramatically lowers the (2PY)­NH stretching frequency to ∼2700 cm^–1. For all clusters the ammonia 2ν₄ HNH bend overtones in the 3180–3320 cm^–1 region gain intensity by anharmonic coupling (Fermi resonance) to the hydrogen-bonded ammonia NH stretches, which are red-shifted into the 3250–3350 cm^–1 region. The experimental results are supported by optimized structures, vibrational frequencies, and IR intensities calculated using density-functional theory with the B3LYP and PW91 functionals, as well as with the more recent functionals B97-D and M06-2X, which are designed to include long-range dispersive interactions

Gas-Phase Cytosine and Cytosine‑N₁‑Derivatives Have 0.1–1 ns Lifetimes Near the S₁ State Minimum

Ultraviolet radiative damage to DNA is inefficient because of the ultrafast S₁ ⇝ S₀ internal conversion of its nucleobases. Using picosecond pump–ionization delay measurements, we find that the S₁(¹<i>ππ</i>*) state vibrationless lifetime of gas-phase keto-amino cytosine (Cyt) is τ = 730 ps or ∼700 times longer than that measured by femtosecond pump–probe ionization at higher vibrational excess energy, <i>E</i>_exc. N₁-Alkylation increases the S₁ lifetime up to τ = 1030 ps for N₁-ethyl-Cyt but decreases it to 100 ps for N₁-isopropyl-Cyt. Increasing the vibrational energy to <i>E</i>_exc = 300–550 cm^–1 decreases the lifetimes to 20–30 ps. The nonradiative dynamics of S₁ cytosine is not solely a property of the amino-pyrimidinone chromophore but is strongly influenced by the N₁-substituent. Correlated excited-state calculations predict that the gap between the S₂(¹<i>n</i>_Oπ*) and S₁(¹<i>ππ</i>*) states decreases along the series of N₁-derivatives, thereby influencing the S₁ state lifetime