1 research outputs found
Direct Electrochemical Measurements of Reactive Oxygen and Nitrogen Species in Nontransformed and Metastatic Human Breast Cells
The production of reactive oxygen
and nitrogen species (ROS and
RNS) in human cells is implicated in various diseases, including cancer.
Micrometer-sized electrodes coated with Pt black and platinized Pt
nanoelectrodes have previously been used for the detection of primary
ROS and RNS in biological systems. In this Article, we report the
development of platinized carbon nanoelectrodes with well-characterized
geometry and use them as scanning electrochemical microscopy (SECM)
tips to measure ROS and RNS inside noncancerous and metastatic human
breast cells. By performing time-dependent quantitative amperometric
measurements at different potentials, the relative concentrations
of four key ROS/RNS in the cell cytoplasm and their dynamics were
determined and used to elucidate the chemical origins and production
rates of ROS/RNS in nontransformed and metastatic human breast cells