1 research outputs found
Antibiotic resistance of Pseudomonas aeruginosa strains isolated from patients
Nicolae Testemitanu State University of Medicine and Pharmacy, Republic of Moldova,
National Agency of Public Health, Chisinau, Republic of MoldovaIntroduction. Antimicrobial resistance represents a global burden and crisis but also a
significant threat to public health nowadays. Healthcare-associated infections with multidrug resistant (MDR) bacteria lead to increased morbidity rates, with a continuous increasing incidence at global levels. The selective pressure created by the widespread use
of antibiotics might be the main explanation of this burden. It is known that P. aeruginosa
is one of the most frequent bacteria involved in the etiology of ventilator-associated pneumonia, with a relative high prevalence in patients admitted to intensive care units and a
high mortality rate. Studies showed that the presence of MDR strains could be an important predictor of hospital death. The aim of this study was to evaluate antimicrobial
resistance patterns of P. aeruginosa strains isolated from various clinical specimens of
hospitalized patients
Material and methods. We conducted a retrospective study during January–December
2020. Isolation of P. aeruginosa strains was performed on selective media for Gram-negative. Identification of strains was done both by conventional methods and by automated
methods using Vitek®2 Compact. Antibiotic susceptibility testing was done using the Vitek®2Compact, but also by Kirby-Bauer method. Interpretation susceptibility was performed according to the EUCAST (European Committee on Antimicrobial Susceptibility
Testing).
Results. They were analyzed 118 isolates from patients hospitalized in surgical wards,
51.2% male, respectively 48.8% women. These strains were isolated from different pathological products: lower tract respirator (39.9%), pus (35.6%), urine (15.4%), central venous catheter insertion (2.7), blood (2.1%) and other secretions (4.3%).
P. aeruginosa strains showed the following levels of antibiotic resistance, respectively,
70.7% to ticarcillin, 58.7% to piperacillin, while resistance to the penicillins combined
with beta-lactamase inhibitors, piperacillin with tazobactam was 43.5%. Resistance to antipseudomonal cephalosporins was 37.9% to ceftazidime and 50.8% to cefepime, while
the level of resistance to carbapenems was 55.9 % to imipenem and 27.1% to meropenem.
Resistance to aminoglycosides was 51.9% to gentamicin, 58.4% to tobramycin and 34.4%
to amikacin. 62.8% of strains showed resistance to fluoroquinolones (ciprofloxacin).
Conclusion. Multidrug resistance is common and increasing. Occurrence of these MDR
strain in clinical care settings makes them difficult and expensive to treat because these
drug resistant strain are exhibit resistance to essentially all reliable antipseudomonal antibiotics. The available clinical solution for antibiotic resistance Pseudomonas aeruginosa
infections requires a precise diagnostic and combination antibiotic therapy based on diagnostics. Judicious administration of antibiotics in combination with nosocomial infection control measures need to be introduced in hospitals to prevent the circulation of these
multidrugresistant strains