A Stable Non-BPS Configuration From Intersecting Branes and Antibranes

We describe a tachyon-free stable non-BPS brane configuration in type IIA string theory. The configuration is an elliptic model involving rotated NS5 branes, D4 branes and anti-D4 branes, and is dual to a fractional brane-antibrane pair placed at a conifold singularity. This configuration exhibits an interesting behaviour as we vary the radius of the compact direction. Below a critical radius the D4 and anti-D4 branes are aligned, but as the radius increases above the critical value the potential between them develops a minimum away from zero. This signals a phase transition to a configuration with finitely separated branes

IN SILICO ANALYSIS OF INHIBITOR AND SUBSTRATE BINDING SITE OF SERRAPEPTIDASE FROM SERRATIA MARCESCENS MTCC 8708

Objective: Serrapeptidase is a therapeutic enzyme broadly used as an anti-inflammatory drug to treat inflammatory diseases like arthritis, bronchitis, fibrocystic breast disease and sinusitis. The objective of present study is in silco analyzes of the substrate and inhibitor binding sites of serratiopeptidase, expressed from a cloned gene.Methods: The gene encoding Serrapeptidase was amplified from genomic DNA of Serratia marcescens MTCC 8707, an isolated from the flowers of summer squash plants. The gene was sequenced, the nucleotide sequence of 1464 nucleotides was submitted to Gen Bank nucleotide database and accession number GI: KP869847 obtained. The develop amino acid sequence was used to predict 3D structure using different bioinformatics tools and software's Further, CABS-dock and Swiss Dock, the docking servers were used for enzyme-substrate/inhibitor binding site analysis. The inflammatory mediators, bradykinin, and substance-P were used as substrates, whereas, EDTA and Lisinopril were used as an inhibitor for serrapeptidase. UCSF Chimera program was used for interactive visualization and analysis of docked results.Results: The docking studies show substrates bradykinin and substance-P bind near zinc binding site with minimum RMSD value and the inhibitors EDTA and lisinopril showed favorable interaction at zinc binding site of serrapeptidase with minimum free energy.Conclusion: The result of docking studies confirm that the substrate or inhibitor binds near zinc binding domain (HEXXH.) and the peptide bond of the substrate can be effectively cleaved by serrapeptidase.Keywords: Serrapeptidase, Anti-inflammation, Arthritis, Molecular docking, Drug discovery, Protein-peptide interaction, Bradykinin, Substance-

Simultaneous Determination of Sitagliptin Phosphate Monohydrate and Metformin Hydrochloride in Tablets by a Validated UPLC Method

A novel approach was used to develop and validate a rapid, specific, accurate and precise reverse phase ultra performance liquid chromatographic (UPLC) method for the simultaneous determination of Sitagliptin phosphate monohydrate and Metformin hydrochloride in pharmaceutical dosage forms. The chromatographic separation was achieved on Aquity UPLC BEH C8 100 × 2.1 mm, 1.7 μm, column using a buffer consisting of 10 mM potassium dihydrogen phosphate and 2 mM hexane-1-sulfonic acid sodium salt (pH adjusted to 5.50 with diluted phosphoric acid) and acetonitrile as organic solvent in a gradient program. The flow rate was 0.2 mL min−1 and the detection wavelength was 210 nm. The limit of detection (LOD) for Sitagliptin phosphate monohydrate and Metformin hydrochloride was 0.2 and 0.06 μg mL−1, respectively. The limit of quantification (LOQ) for Sitagliptin phosphate monohydrate and Metformin hydrochloride was 0.7 and 0.2 μg mL−1, respectively. This method was validated with respect to linearity, accuracy, precision, specificity and robustness. The method was also found to be stability-indicating

Preparation of Electronics grade Bismuth, Antimony, Tellurium, Cadmium and Zinc by Vaccum distillation and Zone refining

APART from the usual importance of bismuth, antimony, tellurium, cadmium & zinc in thechemical, metallurgical, cosmetic, pharmaceutical and paint industries , these materials in their high purity forms (9999 to 99999 %) have recently acquired special significance in the electronics industry for semiconductor and various other applications. The major applications of these electronics grade materials in the electronic industry are : Bismuth : solders and thermo-electric devices, Antimony : semi-conductor devices + for crystal doping), photocell cathodes, Tellurium Compound semi-conductors Cadmium Solders mostly for semi-conductor processing and for compound semi-conductors, such as CdS. Zinc : dopant and diffusant in semi-conductors

Primary structure of Belladonna Mottle virus coat protein

The coat protein of belladonna mottle virus (a tymovirus) was cleaved by trypsin and chymotrypsin, and the peptides were separated by high performance liquid chromatography using a combinatioofn g el permeation, reverse phase, and ion pair chromatography. The peptides were sequenced manually using the 4-N, N-dimethylaminoazobenzene-4′-isothiocyanate/phenyl isothiocyanate double-coupling method. The chymotryptic peptides were aligned by overlapping sequences of tryptic peptides and by homology with another tymovirus, eggplant mosaic virus. The belladonna mottle virus is more closely related to eggplant mosaic virus than to turnip yellow mosaic virus, the type member of this group, as evident from the sequence homologies of 57 and 32%, respectively. The accumulation of basic residues at the amino terminus implicated in RNA-protein interactions in many spherical plant viruses was absent in all the three sequences. Interestingly, the amino-terminal region is the least conserved among the tymoviruses. The longest stretch of conserved sequence between belladonna mottle virus and eggplant mosaic virus was residues 34-44, whereas it was residues 96-102 in the case of belladonna mottle virus and turnip yellow mosaic virus. A tetrapeptide in the region (residues 154-157) was found to be common for all the three sequences. It is possible that these conserved regions (residues 34-44, 96-102, 164-157) are involved in either intersubunit or RNA-protein interactions

TL glow curve and kinetic of gamma irradiated quartz collected from Rasmada mines of C.G. basin

Present paper reports the TL glow curve and kinetic parameter of Quartz. The sample of natural quartz collected from Rasmada mines of C.G. basin is irradiated with gamma source. For gamma exposure Co60 gamma source was used and the exposure of 1 hour to 4 hour was given to the sample with dose 0.5kGy to 2 kGy. TL glow for quartz crystal shows the peaks at temperature 242, 256, 250 and 2520C for doses 2kGy to 0.5 kGy respectively. The powder sample shows TL glow peak at 236, 251, 254 and 2530C respectively. The corresponding activation energy and frequency factor is calculated by peak shape method. The activation energy found to be 0.79 eV for powder and 1.26 eV for quartz crystal, frequency factor is found to be 4X108 to 3X1013sec-1. The maximum activation energy is found for quartz crystal with gamma exposure of 2kGy. The sample shows first and second order kinetics. The sample was characterized by XRD. The Induction coupled plasma activated emission spectra (ICP - AES) analysis was done to find out the percentage of elements in the quartz mineral

Impact of recent cyclone on the marine fishery sector along the east Godavari and Visakhapatnam districts of Andhra Pradesh

A cyclone which lashed along the coastal Andhra Pradesh on the night of 6-11-'96 resulted in the loss of thousands of human lives and materials. The coast of East Godavari and parts of Visakhapatnam districts were the worst hit areas with high velocity winds bringing in huge tidal waves which inundated the fish landing centres

On the rare occurrence of Elagatis bipinnulata off Kakinada coast.

Elagatis bipinnulata commonly called rainbow runners of the family Carangidae is a fish species found usually at or near the surface over reefs or sometimes far offshore in the Indian waters. They are caught occasionaly by hooks and line and gill nets

Open-String Actions and Noncommutativity Beyond the Large-B Limit

In the limit of large, constant B-field (the ``Seiberg-Witten limit''), the derivative expansion for open-superstring effective actions is naturally expressed in terms of the symmetric products *n. Here, we investigate corrections around the large-B limit, for Chern-Simons couplings on the brane and to quadratic order in gauge fields. We perform a boundary-state computation in the commutative theory, and compare it with the corresponding computation on the noncommutative side. These results are then used to examine the possible role of Wilson lines beyond the Seiberg-Witten limit. To quadratic order in fields, the entire tree-level amplitude is described by a metric-dependent deformation of the *2 product, which can be interpreted in terms of a deformed (non-associative) version of the Moyal * product.Comment: 30 pages, harvma