20 research outputs found

    Comparing wheat homoeologs.

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    <p>DNA sequence comparison of wheat homoeologs of <i>AGO802</i> and <i>AGO804</i>. The three wheat <i>AGO802</i> homoeologs are almost 100% identical for the sequence and <i>AGO804</i> has sequence homology of about 98% among its three homoeologs.</p

    Retroposon insertion in the <i>AGO802B</i> gene, in 10 wheat accession with varying reaction to pre-harvest sprouting.

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    <p>Retroposon insertion in the <i>AGO802B</i> gene, in 10 wheat accession with varying reaction to pre-harvest sprouting.</p

    Phylogenetic comparison of ARGONAUTE4_9.

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    <p>Comparison of ARGONAUTE4_9 class of genes using a Phylogenetic tree from wheat, barley, rice, maize, sorghum and Arabidopsis.</p

    Analysis of a retro-transposon insertion in the <i>AGO802B</i> transcripts.

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    <p>Polymorphism as observed on agarose gel in different wheat varieties with varying reaction to pre-harvest sprouting, due to insertion of a retro-transposon insertion in the <i>AGO802B</i> transcripts.</p

    Methylation differences between wheat varieties.

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    <p>DNA extracted from the embryos of mature seeds was digested with <i>Msp</i> I restriction enzyme and probed with 5S rDNA. PHS resistant varieties RL4137, Snowbird, AC Domain, AC Karma show reduced methylation as compared to a PHS susceptible variety AC Andrew. DNA methylation in CDC Teal, a variety with medium PHS reaction was also less, compared to AC Andrew but more than the PHS resistant varieties.</p

    Expression analysis of <i>AGO802</i> and <i>AGO804</i>.

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    <p>Expression of <i>AGO802</i> and <i>AGO804</i> homoeologs in embryos during seed development in Chinese Spring cultivar. QRT-expression analysis was performed at six different stages consisting of ovaries at 5 DAP stages and embryos at 10, 15, 20, 25 and 30 DAP, shown on X-axis of graph as 1–6 respectively.</p

    MOESM11 of Robust genetic transformation of sorghum (Sorghum bicolor L.) using differentiating embryogenic callus induced from immature embryos

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    Additional file 11: Table S7. Effect of l-cysteine and ascorbic acid on post bombardment recovery of DEC tissues and GUS gene expression

    MOESM2 of Stepwise metabolic engineering of Escherichia coli to produce triacylglycerol rich in medium-chain fatty acids

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    Additional file 2: Figure S2. Comparison of three bacterial phosphatidic acid phosphatases (PAPs). A, sequence alignment of bacterial PAPs. B, TFAs produced by the engineered cells harboring empty vector pCDFDuet-1, RjPAP or RoPAP, respectively. C, fatty acid profile of the cellular fatty acids from the engineered E. coli. PgpB, PAP from E. coli MG1655; RoPAP, PAP from R. opacus PD630; RjPAP, PAP from R. jostii RHA1. All data are the means Âą standard deviations from triplicates
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