1 research outputs found
(2<i>R</i>,3<i>S</i>,2″<i>R</i>,3″<i>R</i>)‑Manniflavanone Protects Proliferating Skeletal Muscle Cells against Oxidative Stress and Stimulates Myotube Formation
We
investigated the antioxidative properties of (2<i>R</i>,3<i>S</i>,2″<i>R</i>,3″<i>R</i>)-manniflavanone (MF) using in vitro assays and examined
its effects on myogenesis and lactate-induced oxidative stress in
C2C12 cells. MF was purified from Garcinia buchananii stem bark. H<sub>2</sub>O<sub>2</sub> and oxygen radical absorbance
capacity assays demonstrated that MF is a powerful antioxidant. This
finding was supported by diphenylpicrylhydrazine radical scavenging
activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic
acid and myricetin. Moreover, MF accelerated myotube formation in
the differentiated C2C12 cells by up-regulating myogenic proteins
such as MyoG and myosin heavy chain. Furthermore, MF rescued late
differentiation of myoblast suppressed by lactate treatment and up-regulated
the expression levels of Nrf2 in lactate-induced oxidative stress,
indicating that MF stimulates antioxidative activity inside C2C12
cells. Collectively, MF is a potent antioxidant with a higher safety
profile than ascorbic acid and myricetin. It reduces oxidative stress-induced
delaying of skeletal muscle differentiation by scavenging reactive
oxygen species and regulating myogenic proteins factors