Body length, body weight, spermatophore weight, and total sperm count of wild-caught and domesticated shrimp.

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Overview of liquid-liquid extraction and UHPLC-HRMS/MS analysis of eicosanoids and PUFAs in the <i>P</i>. <i>monodon</i> male reproductive tract.

(A) Male P. monodon broodstocks were dissected to obtain testes (TT) and vas deferens (VD). (B) Sample preparation included tissue homogenization, pH adjustment, addition of antioxidant (10% BHT), and addition of internal standards. (C) Testis homogenates were subjected to ethyl acetate extraction (upper panel) whereas vas deferens homogenates were subjected to methanol-chloroform extraction (lower panel). (D) Tissue extracts were analyzed using the UHPLC system. Eicosanoids and PUFAs were then identified using HRMS/MS. (E) Metabolite quantification and data analysis were performed to determine levels of eicosanoids and PUFAs in each organ. LN2 and ACN were abbreviated for liquid nitrogen and acetonitrile, respectively.</p

Percentage of internal standards recovered from liquid-liquid extractions of <i>P</i>. <i>monodon</i> testes and vas deferens.

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Regression equations for the quantification of PUFAs and eicosanoids in <i>P</i>. <i>monodon</i>.

(DOCX)</p

Criteria for the identification of eicosanoids and PUFAs using retention time, precursor ion, proposed fragment ion, and m/z distribution.

(DOCX)</p

Total sperm counts and percentage of sperm abnormality in domesticated shrimp fed with polychaetes and feed pellets.

Spermatophores of domesticated shrimp fed with either polychaetes (black bar, N = 8) or commercial feed pellets (gray bar, N = 8) were used in the analysis to determine (A) total sperm counts and (B) percentage of sperm abnormality. Error bars represent standard deviations. Asterisks indicate a significant difference between samples using the t-test (** for P < 0.01).</p

Quantitative analysis of eicosanoids and PUFAs in testes and vas deferens of wild-caught and domesticated <i>P</i>. <i>monodon</i>.

Levels of (A) PGE2, (B) PGF2α, (C) 15d-PGJ2, (D) (±)8-HETE, (E) (±)11-HETE, (F) 12(R)-HETE, (G) (±)8-HEPE, (H) (±)12-HEPE, (I) (±)18-HEPE, (J) ARA, (K) DHA, and (L) EPA in testes (TT) and vas deferens (VD) were compared between wild-caught (gray bar, N = 6) and domesticated shrimp (white bar, N = 10). Data are shown as means ± SD. Asterisks indicate statistically significant differences in metabolic levels between wild-caught and domesticated shrimp using the t-test (* for P P t-test († for P P t-test (# for P P < 0.01). ND indicates that the designated metabolite was not detected in this analysis.</p

Sperm count and sperm abnormality in polychaete- and pellet-fed shrimp.

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Comparative analysis of eicosanoid and PUFA levels in testes and vas deferens of eleven-month-old, domesticated shrimp fed with polychaetes or feed pellets.

Levels of (A) PGE2, (B) PGF2α, (C) 15d-PGJ2, (D) (±)8-HETE, (E) (±)11-HETE, (F) 12(R)-HETE, (G) (±)12-HEPE, (H) (±)18-HEPE, (I) ARA, (J) DHA, and (K) EPA were compared between polychaete- (black bar, N = 5) and pellet-fed shrimp (gray bar, N = 5). Error bars represent standard deviations. Asterisks indicate statistically significant differences in metabolic levels between polychaete- and pellet-fed shrimp using the t-test (* for P P t-test († for P P t-test (# for P P < 0.01). ND means that the designated metabolite was not detected.</p

UHPLC-HRMS/MS analysis of testes and vas deferens of polychaete- and pellet-fed shrimp.

(XLSX)</p