6 research outputs found
The migration and the adhesion capacity of BMSCs.
<p>(A) The migration capacity was examined by the transwell migration assays. The BMSCs were attracted with or without SDF-1 on the inserts and were stained with crystal violet (upper). Quantitative data of the migratory BMSCs were counted in 5 high-power fields (lower). MAEC was the positive group. Values are means±SEM. *p<0.05 versus control group. Results are representative of three separate experiments. (B) The adhesion capacity was examined by TNF-α on fibronectin. The adhesion BMSCs on the fibronectin were counted in 8 different fields. MAEC was the positive group. Values are means±SEM. *p<0.05 versus control group. Results are representative of three separate experiments.</p
The mRNA expression levels of ICAM-1 and VCAM-1 in BMSCs.
<p>(A) BMSCs were treated with or without 10 ng/ml VEGF for 24 h. Relative ICAM-1 and VCAM-1 mRNA expression levels were determined by RT-PCR. Values of mRNA amounts were normalized to GAPDH expression and expressed relative to control (n=6). Error bars represent SEM (* P<0.05). (B) Relative ICAM-1 and VCAM-1 protein expression levels were determined by western blotting. Values of protein amounts were normalized to β-actin expression and expressed relative to control (n=3). Error bars represent SEM (* P<0.05). (C) The immunofluorescence analysis of PECAM-1, vWF and KDR expression in BMSCs. BMSCs were stained for KDR, vWF and PECAM-1 with Cy3 and were stained for nucleus with Honchest33258. Scale bars represent 100μm. (D) The FACS analysis of PECAM-1, vWF and KDR expression in BMSCs and the quantification were counted. Isotype control staining was shown in shade in representative histograms (n = 3).</p
Characteristics of BMSCs.
<p>(A) Representative images showed the morphology of BMSCs at passage 1, 3 and 8 under a microscope. Scale bars represent 100μm. (B) Representative images of FACS analysis showed that BMSCs expressed some maker for CD44, c-kit, CD105 and Sca-1. Isotype control staining was shown in shade in representative histograms (n = 3). </p
Additional file 4: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Table S1. Spearman rank correlation coefficients for ASL, BOLD, and IVIM imaging parameters. (DOCX 16 kb
Additional file 3: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Figure S3. Graphs depicting serial measurements of imaging parameters from ASL, BOLD, and IVIM for the anterior, lateral, soleus, and gastrocnemius muscle groups in the control side in healthy old subjects. (PPTX 654 kb
Additional file 2: of Evaluation of skeletal muscle microvascular perfusion of lower extremities by cardiovascular magnetic resonance arterial spin labeling, blood oxygenation level-dependent, and intravoxel incoherent motion techniques
Figure S2. Graphs depicting serial measurements of imaging parameters from ASL, BOLD, and IVIM for the anterior, lateral, soleus, and gastrocnemius muscle groups in the control side in healthy young subjects. (PPTX 727 kb