14 research outputs found
pH-Stable Zn(II) Coordination Polymer as a Multiresponsive Turn-On and Turn-Off Fluorescent Sensor for Aqueous Medium Detection of Al(III) and Cr(VI) Oxo-Anions
Nowadays, coordination polymers (CPs)
are promising candidates
as sensory materials for their high sensitivity, improved selectivity,
fast responsive nature, as well as good recyclability. However, poor
chemical stability often makes their practical usage limited. Herein,
employing a mixed ligand approach, we constructed a chemically robust
CP, {[Zn2L2(DPA)2]·3H2O}n (IITKGP-70, IITKGP stands
for the Indian Institute of Technology Kharagpur), which exhibited
excellent framework robustness not only in water but also over a broad
range of pH solutions (pH = 3–11). The developed framework
displayed high selectivity and sensitivity for the detection of trivalent
Al3+ ions and toxic hexavalent Cr(VI)-oxo anions in an
aqueous medium. The developed framework exhibited
an aqueous medium Al3+ turn-on phenomenon with a limit of detection (LOD) value of 1.29 μM,
whereas a turn-off effect was observed for toxic
oxo-anions (Cr2O72– and CrO42–) having LOD values of 0.27 and 0.71 μM,
respectively. Both turn-on and turn-off mechanisms are speculated via spectroscopic methods
coupled with several ex situ studies. Such a multiresponsive nature (both turn-on and turn-off) for aqueous medium detection
of targeted cations and anions simultaneously in a single platform
coupled with high robustness, ease of scalability, recyclability,
and fast-responsive nature makes IITKGP-70 highly fascinating
as a sensory material for real-world applications
Tunable Color in 2,6-Diaminopyridine-Functionalized Graphene Oxide
Graphene oxide (GO)
is functionalized by 2,6-diaminopyridine (DAP)
to invoke a superior optical property. Temperature-dependent photoluminescence
has been carried out to elucidate the hybridized energy levels generated
due to functionalization of GO. In addition to a blue color at 300
K, a new peak in the wavelength region of 500–700 nm is observed
as the temperature decreases to 78 K. Density functional theory calculations
are carried out to estimate the hybridized energy levels arising due
to functionalization. This tunable color in functionalized graphene
oxide is very useful in designing light-emitting devices. A light-emitting
diode is fabricated using this DAP-functionalized graphene oxide (DAP–fGO)
as an emissive material. Tunable electroluminescence in the visible
range (red to green) with increasing bias voltage and finally white
emission are obtained from the device at an operating voltage above
8 V
Anti-Inflammatory Activity of <i>Odina wodier</i> Roxb, an Indian Folk Remedy, through Inhibition of Toll-Like Receptor 4 Signaling Pathway
<div><p>Inflammation is part of self-limiting non-specific immune response, which occurs during bodily injury. In some disorders the inflammatory process becomes continuous, leading to the development of chronic inflammatory diseases including cardiovascular diseases, diabetes, cancer etc. Several Indian tribes used the bark of <i>Odina wodier</i> (OWB) for treating inflammatory disorders. Thus, we have evaluated the immunotherapeutic potential of OWB methanol extract and its major constituent chlorogenic acid (CA), using three popular <i>in vivo</i> antiinflammatory models: Carrageenan- and Dextran-induced paw edema, Cotton pellet granuloma, and Acetic acid-induced vascular permeability. To elucidate the possible anti-inflammatory mechanism of action we determine the level of major inflammatory mediators (NO, iNOS, COX-2-dependent prostaglandin E2 or PGE2), and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, and IL-12). Further, we determine the toll-like receptor 4 (TLR4), Myeloid differentiation primary response gene 88 (MyD88), c-Jun N-terminal kinases (JNK), nuclear factor kappa-B cells (NF-κB), and NF-kB inhibitor alpha (IK-Bα) by protein and mRNA expression, and Western blot analysis in drug treated LPS-induced murine macrophage model. Moreover, we determined the acute and sub-acute toxicity of OWB extract in BALB/c mice. Our study demonstrated a significant anti-inflammatory activity of OWB extract and CA along with the inhibition of TNF-α, IL-1β, IL-6 and IL-12 expressions. Further, the expression of TLR4, NF-κBp65, MyD88, iNOS and COX-2 molecules were reduced in drug-treated groups, but not in the LPS-stimulated untreated or control groups, Thus, our results collectively indicated that the OWB extract and CA can efficiently inhibit inflammation through the down regulation of TLR4/MyD88/NF-kB signaling pathway.</p></div
Effect of OWB extract on Carrageenan- and Dextran- induced paw edema, and Cotton pellet-induced granuloma in rats.
<p>(A and C) Inflammation in the right hind paw of Wistar rats was made by subcutaneous injection of carrageenan or dextran respectively, under the sub plantar aponeurosis. The test groups were administered with 200 or 400 mg kg<sup>−1</sup> of OWB extract orally, 1 h before carrageenan or dextran injection; while the control group received distilled water or Indomethacin (10 mg/kg). After 6 h, the paw volume was measured and % of inhibition was compared with the control groups. (B) Photographs showing Carrageenan-induced Paw edema (CIPE) in the hind limb of rats, 6 h after carrageenan challenge. Redness and swelling of paw are evident with respect to control and OWB extract treatment. (D) Subcutaneously implemented sterile cotton-pellets (10 mg each) in the axilla regions of the rats, under anesthesia, were treated orally with the extract (200 or 400 mg kg<sup>−1</sup>) daily for 7 consecutive days, with respect to the normal saline or Indomethacin (10 mg/kg). After scarification, on 8<sup>th</sup> day, the cotton-pellets were removed, cleaned and the dry weight of each pellet was taken to calculate the percentage of inhibition with respect to the cotton-pellet weight, compared with the control. Results are expressed as Mean ± SD, (n = 6), *, <i>P</i>P</p
Effect of OWB extract and CA on the JNK, MAPK, IkB-α and MyD88 expression.
<p>Expression of (A) JNK, (B) MAPK, (C) IkB-α and (D) MyD88 were determined by the Western blot, using GAPDH as the internal control. The LPS (1 µg/ml) induced peritoneal macrophage(s) were treated with OWB (100 µg/ml) or CA (10 µg/ml), and after 24 h the protein extract from whole cell were harvested in buffer, containing 20 mM Tris (pH 7±0.5), 50 mM NaCl, 5% NP-40 and 0.05% DOC. The soluble fraction was separated by centrifugation, subjected to SDS-PAGE and blotted to pre-equilibrated PVDF membrane. The membrane was then blocked in 5% NFDM in 1X TBST, rinsed and incubated with specific antibody at 4°C overnight. Immunoblotting was performed with peroxidase-labelled specific antibodies and visualized by ECL Western blot detection kit. The average expression of NF-kB and MAPK was significantly higher in the LPS-induced macrophage, as compared to the control and OWB or CA co-treated group (** <i>P</i></p
Effect of OWB extract and CA on pro-inflammatory and anti-inflammatory cytokine release in LPS-induced peritoneal macrophages by sandwich ELISA and RT-PCR.
<p>Peritoneal macrophages were cultured overnight and incubated with LPS (1 µg/ml), washed after 4 h and then treated with the OWB (50 or 100 µg/m) or CA (10 µg/ml). The cells were further incubated for 24 h, and the cell-free supernatants were subjected to sandwich ELISA to determine the level of (A) TNF-α, (C) IL-1β, (E) IL-6 and (G) IL-6 (pg/mL). In a separate set similarly treated cells were cultured for 5 h, and collected in TRI Reagent for mRNA extraction and subsequent RT–PCR analysis (vide Materials and methods) to study the cytokine and β-actin mRNA expression. The data were shown for the expression of (B) TNF-α, (D) IL-1β, (F) IL-6 and (H) IL-12. The ELISA and RT–PCR data are expressed as Means ± SD from triplicate experiments, yielding similar results. Asterisks indicate statistically significant (**P, 0.05) induction of TNF-α, IL-1β, IL-6, and IL-12 release; and increase or decrease (**P, 0.05; *P, 0.001) in cytokine expression, compared to the infected macrophages.</p
Effect of OWB extract and CA on TLR4 and MyD88 expression.
<p>The LPS-stimulated macrophages were treated with the OWB or CA and incubated for 24 h, following which RNA was isolated for RT–PCR analysis of the expression of MyD88 (A) and TLR4 (B) mRNA. The RT–PCR data are expressed as Means ± SD from triplicate experiments. The expression of TLR4 and MyD88 was significantly higher in the LPS-induced macrophage as compared to the control and OWB or CA co-treated group (** <i>P</i></p
Immunomodulatory activity of OWB extract and CA via nitrite generation and iNOS2 expression in LPS-induced murine macrophages.
<p>(A) Macrophages (10<sup>6</sup> cells/ml) were incubated with LPS (1 µg/ml), OWB (50 and 100 µg/ml) or CA (10 µg/ml) for 24 h. The cell-free supernatants were collected for nitrite assay, as described in the Materials and methods. Data were expressed as Means ± SD from triplicate experiments, yielding similar results (µ moles of nitrite). Asterisks indicate a statistically significant increase (**P, 0.05) in nitrite generation, compared to the infected macrophages. (B–C) The LPS-stimulated macrophages were treated with the OWB extract and CA, incubated for 24 h, following which RNA was isolated and subjected to the RT–PCR analysis for the expression of iNOS2 mRNA. The data were expressed as Mean ± SD from triplicate experiments yielding similar results. The asterisk indicates a statistically significant increase (**P, 0.001) in iNOS2 expression, compared to the infected macrophages.</p
Effect of OWB extract and CA on protein denaturation.
<p>Each value represents the Mean ± SD; n = 6) * p<0.001** p<0.01.</p