1 research outputs found
Antimicrobial effect of the extracts from Hypericum perforatum against oral bacteria and biofilm formation
Context: One traditional medicines, Hypericum perforatum L.
(Hypericaceae), possesses several beneficial effects against depression,
ulcers, dyspepsia, abdominal pains, burns, bacterial infections,
migraine headaches, and sciatica.
Objective: The present study investigates the antimicrobial activity of
the ethanol extract (HP-EtOH) of H. perforatum and its sub-extracts,
namely n-hexane (HP-hexane), chloroform (HP-CHCl3), ethyl acetate
(HP-EtOAc), n-butanol (HP-n-BuOH), and water (HP-H2O) extracts, against
Streptococcus mutans, S. sobrinus, Lactobacillus plantarum, and
Enterococcus faecalis.
Materials and methods: For the evaluation of the antimicrobial activity,
flowering aerial parts of H. perforatum were extracted with EtOH and
then this extract was fractionated to obtain five sub-extracts in
different polarities. Antimicrobial activities of HP-EtOH and its
sub-extracts against Streptococcus mutans, S. sobrinus, L. plantarum,
and E. faecalis were assessed by using colorimetric micro-well dilution
at concentration ranges of 64-0.5 mu g/ml as well as resazurin
microplate and modified microtiter-plate assays between the ranges of
100 and 0.78125 mu g/ml.
Results: According to the results of the present study, HP-H2O
sub-extract displayed strong antibacterial activity (MIC values 8 mu
g/mL) against S. sobrinus and L. plantarum, and exerted moderate
activity against S. mutans and E. faecalis at 32 and 16 mu g/mL
concentrations, respectively. Other sub-extracts also demonstrated
antimicrobial activity against S. sobrinus at a concentration of 16 mu
g/mL. HP-EtOAc and HP-n-BuOH showed antimicrobial activity against L.
plantarum and HP-EtOAc and HP-H2O were also active against E. faecalis
at the same concentrations (16 mu g/mL).
Conclusion: According to the results, we suggest that H. perforatum
could be employed as a natural antibacterial agent in oral care
products