43 research outputs found

    Natural attenuation of arsenic by sediment sorption and oxidation

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    Arsenic sorption onto aquifer sediments was investigated in anaerobic laboratory batch and column uptake experiments and characterized by As, Fe, and Mn X-ray absorption spectroscopy (XAS) to estimate the extent and mechanism of abiotic sorption and oxidation of As(III). Batch experiments at pH 6 showed that the amount of As(III) or As(V) sorption from synthetic background porewater to sediments was similar as a function of total As concentration, but slightly more As(V) was sorbed than As(III) with increasing As concentrations. Column experiments with As(III) solutions in the absence and presence of dissolved Fe^2+ showed more As uptake in the presence of Fe but also more Fe desorption during flushout with As-free solutions such that net As uptake was similar to, or less than that of, the Fe-free experiment. Fits to bulk Fe X-ray absorption near-edge spectroscopy (XANES) spectra showed no change between unreacted and reacted sediments. Manganese XANES revealed small increases in absorption in the spectral region associated with Mn(II) after reaction, indicating sediment Mn reduction. However, XANES spectra showed that Mn is not present as Mn^(IV)O_2(s) but is probably substituted into other sediment minerals as a mixture of Mn(II,III). Quantitative analyses of As XANES spectra, which indicated mixtures of As(III) and As(V) after reaction with As(III) solutions, were used to estimate a fraction of As(V) in excess of native As(V) in the sediment (0.2 mmol kg^−1) that corresponds to sorbed As(III) oxidized to As(V). The spectroscopic and solution data indicate that the aquifer sediments have a limited abiotic capacity to oxidize As(III), which did not exceed 30% of the total amount of As sorbed and was estimated in the range of 0.025−0.4 mmol kg^−1 sediment. In the presence of dissolved Fe^2+, the precipitation of Fe(III) hydrous oxide phases will be an effective mechanism for As scavenging only if there exists sufficient dissolved oxygen in groundwater to oxidize Fe. Once the aqueous oxidative capacity is exhausted, dissolved Fe^2+ may compete with As(III) for the limited abiotic oxidation supplied by sediment Mn-bearing phases

    Generating reliable tourist accommodation statistics: Bootstrapping regression model for overdispersed long-tailed data

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    Purpose: Few studies have applied count data analysis to tourist accommodation data. This study was undertaken to investigate the characteristics and to seek for the most fitting models for population total estimation in relation to tourist accommodation data. Methods: Based on the data of 10,503 hotels, obtained from by a nationwide Japanese survey, the bootstrap resampling method was applied for re-randomisation of the data. Training and test sets were derived by randomly splitting each of the bootstrap samples. Six count models were fitted to the training set and validated with the test set. Bootstrap distributions for parameters of significance were used for model evaluation. Results: The outcome variable (number of guests), was found to be heterogenous, over dispersed and long-tailed, with excessive zero counts. The hurdle negative binomial and zero-inflated negative binomial models outperformed the other models. The accuracy (se) of the estimation of total guests with training sets that ranged from 5% to 85%, was from 3.7 to 0.4 respectively. Results appear little overestimated. Implications: Findings indicated that the integration of the bootstrap resampling method and count regression provide a statistical tool for generating reliable tourist accommodation statistics. The use of bootstrap would help to detect and correct the bias of the estimation

    RBFOX3 regulates Claudin-1 expression in human lung tissue via attenuation of proteasomal degradation

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    RBFOX3, a nuclear RNA-binding protein, is well known as a regulator of alternative pre-mRNA splicing during neuronal development. However, other functions of RBFOX3 are poorly understood. Here, we investigated the function of RBFOX3 in the cytoplasm with respect to regulation of Claudin-1 expression. In human lung tissue, Claudin-1 is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 are increased by RBFOX3. In addition, cycloheximide treatment of human lung cancer cells revealed that RB-FOX3 increases the stability of Claudin-1 through attenuation of its ubiquitination. Our study provides insights into the molecular mechanisms by which RBFOX3 regulates Claudin-1 expression in human lung tissue
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