The critical role of intracellular zinc in adenosine A2 receptor activation induced cardioprotection against reperfusion injury

Exogenous zinc can protect cardiac cells from reperfusion injury, but the exact roles of endogenous zinc in the pathogenesis of reperfusion injury and in adenosine A2 receptor activation-induced cardioprotection against reperfusion injury remain unknown. Adenosine A1/A2 receptor agonist 5′-(N-ethylcarboxamido) adenosine (NECA) given at reperfusion reduced infarct size in isolated rat hearts subjected to 30 min ischemia followed by 2 h of reperfusion. This effect of NECA was partially but significantly blocked by the zinc chelator N,N,N′,N′-tetrakis-(2-pyridylmethyl) ethylenediamine (TPEN), and ZnCl2 given at reperfusion mimicked the effect of NECA by reducing infarct size. Total tissue zinc concentrations measured with inductively coupled plasma optical emission spectroscopy (ICPOES) were decreased upon reperfusion in rat hearts and this was reversed by NECA. NECA increased intracellular free zinc during reperfusion in the heart. Confocal imaging study showed a rapid increase in intracellular free zinc in isolated rat cardiomyocytes treated with NECA. Further experiments revealed that NECA increased total zinc levels upon reperfusion in mitochondria isolated from isolated hearts. NECA attenuated mitochondrial swelling upon reperfusion in isolated hearts and this was inhibited by TPEN. Similarly, NECA prevented the loss of mitochondrial membrane potential (ΔΨm) caused by oxidant stress in cardiomyocytes. Finally, both NECA and ZnCl2 inhibited the mitochondrial metabolic activity. NECA-induced cardioprotection against reperfusion injury is mediated by intracellular zinc. NECA prevents reperfusion-induced zinc loss and relocates zinc to mitochondria. The inhibitory effects of zinc on both the mPTP opening and the mitochondrial metabolic activity may account for the cardioprotective effect of NECA

Yoga Training Improves Metabolic Parameters in Obese Boys

Yoga has been known to have stimulatory or inhibitory effects on the metabolic parameters and to be uncomplicated therapy for obesity. The purpose of the present study was to test the effect of an 8-week of yoga-asana training on body composition, lipid profile, and insulin resistance (IR) in obese adolescent boys. Twenty volunteers with body mass index (BMI) greater than the 95th percentile were randomly assigned to yoga (age 14.7±0.5 years, n=10) and control groups (age 14.6±1.0 years, n=10). The yoga group performed exercises three times per week at 40~60% of heart-rate reserve (HRR) for 8 weeks. IR was determined with the homeostasis model assessment of insulin resistance (HOMA-IR). After yoga training, body weight, BMI, fat mass (FM), and body fat % (BF %) were significantly decreased, and fat-free mass and basal metabolic rate were significantly increased than baseline values. FM and BF % were significantly improved in the yoga group compared with the control group (p\u3c0.05). Total cholesterol (TC) was significantly decreased in the yoga group (p\u3c0.01). HDL-cholesterol was decreased in both groups (p\u3c0.05). No significant changes were observed between or within groups for triglycerides, LDL-cholesterol, glucose, insulin, and HOMA-IR. Our findings show that an 8-week of yoga training improves body composition and TC levels in obese adolescent boys, suggesting that yoga training may be effective in controlling some metabolic syndrome factors in obese adolescent boys

Hard–Soft Cooperativity of an Allosteric Tweezer

Drawing inspiration from allosteric proteins, a zigzag-shaped π-conjugation was structurally engineered into a tweezer-like ionophore having multiple disparate binding sites. When a soft metal ion binds to the central tridentate ligand motif, the rigid backbone folds, bringing two macrocyclic arms into close proximity. Stabilized by a coordinating anion, the tweezer-like conformation of the resulting metalloligand recruits a hard cation to form a sandwich-like complex with a remarkably enhanced binding affinity and selectivity

Application of Stage-Fall-Discharge Rating Curves to a Reservoir Based on Acoustic Doppler Velocity Meter Measurement Data

The applicability of the stage-fall-discharge (SFD) method in combination with acoustic Doppler velocity meter (ADVM) data, upstream of a hydraulic structure, specifically, the Sejong-weir located in the Geum River, Korea, was examined. We developed three rating curves: a conventional simple rating curve with the data measured using an acoustic Doppler current profiler (ADCP) and floating objects, an SFD rating curve with the data measured using the ADCP and floating objects, and an SFD rating curve with the data measured using an ADVM. Because of the gate operation effect, every rating curve involved many uncertainties under 1000 m3/s (3.13 m2/s, specific discharge). In terms of the hydrograph reconstruction, compared with the conventional simple rating curve, the SFD developed using ADVM data exhibited a higher agreement with the measured data in terms of the pattern. Furthermore, the measured discharge over 1000 m3/s primarily ranged between 97.5% and 2.5% in the graph comparing the ratio of the median and observed discharge. Based on this experiment, it is confirmed that the SFD rating curve with data to represent the backwater effect, such as ADVM data, can reduce the uncertainties induced by the typical rating curv

Curved TVs improved watching experience when display curvature radii approached viewing distances: Effects of display curvature radius, viewing distance, and lateral viewing position on TV watching experience

Although watching TV often involves multiple viewing distances and viewers, less attention has been paid to the effects of display curvature radius, viewing distance, and lateral viewing position on TV watching experience. This study examined the effects of four display curvature radii (2300R, 4000R, 6000R, and flat), two viewing distances (2.3 m and 4 m), and five lateral viewing positions (P-1-P-5; 0, 35, 70, 105, and 140 cm off-center) on seven TV watching experience elements (spatial presence, engagement, ecological validity, negative effects, visual comfort, image quality, and user satisfaction). Fifty-six individuals (14 per display curvature radius) were seated in pairs to watch videos, each time at a different viewing position (2 viewing distances x 5 paired lateral viewing positions). The spatial presence and engagement increased when display curvature radius approached a viewing distance and lateral viewing position approached P-1, with 4000R-4m-P-1 (display curvature radius-viewing distance-lateral viewing position) providing the best results. Lateral viewing position alone significantly affected five TV watching experience elements; the spatial presence and engagement decreased at P-3-P-5, and ecological validity, image quality, and user satisfaction decreased at P-4-P-5. However, display curvature radius alone did not appreciably affect TV watching experience, and viewing distance alone significantly affected visual comfort only, with a 4-m viewing distance increasing visual comfort. This study demonstrated that effective display curvature radii for watching TV are viewing distance-dependent, and less off-center lateral viewing positions (P-1-P-2) are recommended for TV watching experience. Finally, among the TV watching experience elements, engagement explained user satisfaction to the greatest degree