Review on assessing climate-change-induced risks to run-of-river hydropower infrastructure in Nepal

Nepal annually invests millions of dollars in hydropower development, with a substantial portion of these funds allocated to civil infrastructure. Climate change, which impacts geological and hydrological conditions, poses a threat to civil components throughout hydropower project construction and operation. This causes budget overruns, project delays, and adverse consequences for society and the environment, ultimately resulting in multimillion-dollar losses. The identification of risk factors and their underlying causes, stemming from the impacts of climate change, constitutes a fundamental aspect of this study. This critical analysis primarily draws upon extensive literature reviews to pinpoint these factors (risk factors arising from climate change), emphasizing their economic and human impacts. This paper highlights the risks to run-of-river hydropower infrastructures in Nepal. The findings of the study can be used to develop and implement adaptation strategies to mitigate the risks posed by climate change to Nepal's vital hydropower sector.</p

Detection of Latent HIV-1 Infection and Drug Resistant Mutation Testing in Nepal: HIV-1 env V3 DNA Sequence and RT Gene (M184V) Mutation

HIV-1 resistance to antiretroviral therapy (ART) is a crucial issue, despite various effective drugs are available for the treatment. Although the viral RNA is suppressed below the detection limit (&lt;50 copies/ml) with the use of potent antiviral drugs, the mutation can be archived in the cellular reservoir as proviral DNA. The detection of proviral DNA and mutation screening in HIV 1 RNA for genotypic resistance is the sole basis for monitoring the effectiveness of ART. Our study aim to access the extent of latent HIV infections by detecting env V3 DNA and also testing of M184V (meth184val; ATG - GTG substitution at 184th codon) specific mutations in HIV-1 RT gene to monitor the effectiveness of ART. The HIV-1 env V3 DNA sequence was amplified using multiple upstream and downstream primes to show the latent HIV infections, whereas polymerase chain reaction- restriction fragment digestion assay (PCR-RFDA) was used for testing M184V mutation in HIV-1 RT gene. In the study, out of 15 HIV infected patient blood samples, 12 shows amplification of env V3 DNA, confirming the latent HIV infections while 3 were negative for env V3 DNA. HIV-1 RT gene tested for M184V mutation in all 15 samples showed wild type after analysis using PCR-RFDA. After digestion with CviAII, three bands were observed in wild type whereas in mutant only two bands. Although the study shows negative for the M184V resistance mutation, screening of various panels of drug resistance mutations should be performed in recently infected HIV-1 patients for planning the effective ART strategy. The data is not enough to compare the overall scenario of the Nepal thus warrant urgency for large scale study with standard genotypic tools

Backwater effect in lowland regions due to bridge structure: a case study of Shreekhandapur, Kavre, Nepal

The backwater effect can be caused by a number of factors, including the bridge's pier width and deck level. In lowland regions, the backwater effect can be particularly problematic, as it can lead to flooding and inundation of farmlands and houses. This study examines the impact of bridge structure on the backwater effect in the lowland region of Shreekhandapur, Kavre, Nepal. The study area is characterized by a hill slope and inner river valley, with a gentle river profile that results in low flow velocity and increased deposition. The study compares the backwater effect of an old demolished bridge with four piers of 1.2 m width and abutments on both sides to a new bridge with a single pier of 0.4 m width and a deck level that is 1 m higher. HEC-RAS software was used to analyse the steady flow and create a flood hazard map. The results show that the new bridge with a reduced pier width and increased deck level significantly reduces the backwater effect. The flood hazard map shows that the new bridge along with floodwalls reduces the potential hazard-prone areas by up to 50 %. The findings of this study can be applied to disaster mitigation and bridge design in lowland regions. By reducing pier width and increasing deck level, bridges can be designed to minimize the backwater effect and reduce the risk of flooding.</p

Curcumin β-D-Glucuronide Modulates an Autoimmune Model of Multiple Sclerosis with Altered Gut Microbiota in the Ileum and Feces

ウコンに含まれる成分が腸内フローラを介して脳・脊髄の炎症を抑制 --プロドラッグ型「クルクミン」の多発性硬化症治療への応用に期待--. 京都大学プレスリリース. 2021-12-03.We developed a prodrug type of curcumin, curcumin monoglucuronide (CMG), whose intravenous/intraperitoneal injection achieves a high serum concentration of free-form curcumin. Although curcumin has been reported to alter the gut microbiota and immune responses, it is unclear whether the altered microbiota could be associated with inflammation in immune-mediated diseases, such as multiple sclerosis (MS). We aimed to determine whether CMG administration could affect the gut microbiota at three anatomical sites (feces, ileal contents, and the ileal mucosa), leading to suppression of inflammation in the central nervous system (CNS) in an autoimmune model for MS, experimental autoimmune encephalomyelitis (EAE). We injected EAE mice with CMG, harvested the brains and spinal cords for histological analyses, and conducted microbiome analyses using 16S rRNA sequencing. CMG administration modulated EAE clinically and histologically, and altered overall microbiota compositions in feces and ileal contents, but not the ileal mucosa. Principal component analysis (PCA) of the microbiome showed that principal component (PC) 1 values in ileal contents, but not in feces, correlated with the clinical and histological EAE scores. On the other hand, when we analyzed the individual bacteria of the microbiota, the EAE scores correlated with significant increases in the relative abundance of two bacterial species at each anatomical site: Ruminococcus bromii and Blautia (Ruminococcus) gnavus in feces, Turicibacter sp. and Alistipes finegoldii in ileal contents, and Burkholderia spp. and Azoarcus spp. in the ileal mucosa. Therefore, CMG administration could alter the gut microbiota at the three different sites differentially in not only the overall gut microbiome compositions but also the abundance of individual bacteria, each of which was associated with modulation of neuroinflammation

Sustained Viremic Control in HIV-Infected Patient: Case Report from Nepal

A case of human immunodeficiency virus (HIV) infection is described from Nepal with constant maintenance of CD4 count and HIV-RNA level below the lower detection threshold for more than ten years. The case was diagnosed of HIV positive in the year 2008. He had his viral load estimation performed every year since then which was always below lower detection limit and remained healthy without treatment. The patient also had not any kinds of opportunistic infection till date. He is married now and has not transmitted the disease to his wife

Everest tourism: forging links to sustainable mountain development. A critical discourse on politics of places and peoples

This paper explores the relationships between tourism interventions and the changes in socioeconomic power relations of stakeholders of Everest tourism. The consequences of tourism intervention have been analyzed and interpreted through dialectical phenomenology. Political ecology framework is used as an analytical model for inductive logic generation from the observations of social interactions on Everest tourism. Impacts of tourism interventions on local environment, culture, livelihood of the indigenous people and overall socioeconomic power relations of the people in this region have been interpreted and discussed with the theoretical perspectives of political economy and bio-environmental relationships. Through critical realism on political ecology it is concluded that the interventions of Everest tourism cannot assure the sustainability of the indigenous society, environment and economy of the region. The findings are interpreted in the line of rhetoric on Ecological Modernization Theory

Sero-prevalance of Cryptococcal Antigenemia in HIV Positive Individual having CD4 Counts

Cryptococcus neoformans is one of the foremost common opportunistic infectious agents in people living with Acquired Immuno Deficiency Syndrome (AIDS). It has been reported to cause about 1 million cases of cryptococcal meningitis per year among HIV/AIDS and 600,000 deaths annually. This study was done to find the prevalence of Cryptococcal antigenemia among HIV positive individuals having CD4counts &lt;100 cells/mm3. A cross-sectional study was conducted in the HIV Reference unit, National public health laboratory from July to December 2015. The study comprised of 99 HIV positive individuals having CD4counts &lt;100 cells/mm3. CD4 T cell count was performed by flow cytometry (BD Biosciences, San Jose, CA, USA) and Cryptococcal antigen test by Latex agglutination assay. The overall prevalence of cryptococcal antigenemia was found to be 18.2%. Of the total ninety-nine subjects enrolled in the study, 72 (72.8%) were males and 27 (27.2%) were females. The mean age of the patients was 38 years ranging from 13 to 69 years. Higher percentage of female (22.2%) showed Cryptococcal infection in our study as compared to male (16.7%). The study concludes higher prevalence of Cryptococcal antigenemia among HIV infected individuals and recommends Cryptococcal antigen screening to be made mandatory in HIV positive patients having CD4 T cells count below 100/μl

Curcumin β-D-glucuronide modulates an autoimmune model of multiple sclerosis with altered gut microbiota in the ileum and feces

近畿大学Kindai University博士（医学）主査：永井義隆 教授　　学内授与番号：医第1389号 Sundar Khadka, Seiichi Omura, Fumitaka Sato, Kazuto Nishio, Hideaki Kakeya, Ikuo Tsunoda"Curcumin β-D-glucuronide modulates an autoimmune model of multiple sclerosis with altered gut microbiota in the ileum and feces" Frontiers in Cellular and Infection Microbiology, Volume 11, 2021, p.772962. https://doi.org/10.3389/fcimb.2021.772962 掲載doctoral thesi

Curcumin β-D-glucuronide modulates an autoimmune model of multiple sclerosis with altered gut microbiota in the ileum and feces

主査：永井義隆 教授　　学内授与番号：医第1389号 Sundar Khadka, Seiichi Omura, Fumitaka Sato, Kazuto Nishio, Hideaki Kakeya, Ikuo Tsunoda"Curcumin β-D-glucuronide modulates an autoimmune model of multiple sclerosis with altered gut microbiota in the ileum and feces" Frontiers in Cellular and Infection Microbiology, Volume 11, 2021, p.772962. https://doi.org/10.3389/fcimb.2021.772962 掲