Gene Expression Profile Changes After Short-activating RNA-mediated Induction of Endogenous Pluripotency Factors in Human Mesenchymal Stem Cells

It is now recognized that small noncoding RNA sequences have the ability to mediate transcriptional activation of specific target genes in human cells. Using bioinformatics analysis and functional screening, we screened short-activating RNA (saRNA) oligonucleotides designed to target the promoter regions of the pluripotency reprogramming factors, Kruppel-like factor 4 (KLF4) and c-MYC. We identified KLF4 and c-MYC promoter-targeted saRNA sequences that consistently induced increases in their respective levels of nascent mRNA and protein expression in a time- and dose-dependent manner, as compared with scrambled sequence control oligonucleotides. The functional consequences of saRNA-induced activation of each targeted reprogramming factor were then characterized by comprehensively profiling changes in gene expression by microarray analysis, which revealed significant increases in mRNA levels of their respective downstream pathway genes. Notably, the microarray profile after saRNA-mediated induction of endogenous KLF4 and c-MYC showed similar gene expression patterns for stem cell- and cell cycle-related genes as compared with lentiviral vector-mediated overexpression of exogenous KLF4 and c-MYC transgenes, while divergent gene expression patterns common to viral vector-mediated transgene delivery were also noted. The use of promoter-targeted saRNAs for the activation of pluripotency reprogramming factors could have broad implications for stem cell research

Early childhood care and education in the Asia Pacific Region: Moving towards goal 1

The Report commissioned by UNESCO Asia-Pacific Bureau for Education for the World Conference on Early Childhood Care and Education (WCECCE), 2010. Published jointly by UNESCO Asia-Pacific Regional Bureau for Education and the Comparative Education Research Centre, The University of Hong Kong.The Report is part of the CERC Monograph Series in Comparative and International Education and Development, v. 8. E-text at: http://unesdoc.unesco.org/images/0019/001905/190526e.pd

Scaffolding interactions with preschool children: Comparisons between Chinese mothers and teachers across different tasks

This study investigated how Chinese adults adjusted their scaffolding in interactions with children during problem-solving tasks. Fifty-seven 5-year-olds (from low and high socioeconomic status [SES] backgrounds) completed a playlike task (puzzle) and a school-like task (worksheet) with their mothers and teachers, respectively. Adult-child interactions were videotaped, and the analyses focused on how adults adjusted their scaffolding as a function of children's responses. Results indicated that teachers adjusted their scaffolding more appropriately than mothers, and that adults' sensitivity to children's understanding was associated with more appropriate scaffolding. Children from middle or upper SES families received more optimal scaffolding than those from lower SES families, and both mothers and teachers provided more skilled scaffolding in the school-like task than in the playlike task. Findings suggest that family SES, adults' professional training, and task characteristics, as well as adults' understanding of children's mastery, should be considered in the analysis of dyadic problem solving. © 2012 by Wayne State University Press.published_or_final_versio

Quality early childhood care and education in low resource level countries in Asia

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Observing collapse in two colliding dipolar Bose-Einstein condensates

We study the collision of two Bose-Einstein condensates with pure dipolar interaction. A stationary pure dipolar condensate is known to be stable when the atom number is below a critical value. However, collapse can occur during the collision between two condensates due to local density fluctuations even if the total atom number is only a fraction of the critical value. Using full three-dimensional numerical simulations, we observe the collapse induced by local density fluctuations. For the purpose of future experiments, we present the time dependence of the density distribution, energy per particle and the maximal density of the condensate. We also discuss the collapse time as a function of the relative phase between the two condensates.Comment: 6 pages, 7 figure

Graphene formed on SiC under various environments: Comparison of Si-face and C-face

The morphology of graphene on SiC {0001} surfaces formed in various environments including ultra-high vacuum, 1 atm of argon, and 10^-6 to 10^-4 Torr of disilane is studied by atomic force microscopy, low-energy electron microscopy, and Raman spectroscopy. The graphene is formed by heating the surface to 1100 - 1600 C, which causes preferential sublimation of the Si atoms. The argon atmosphere or the background of disilane decreases the sublimation rate so that a higher graphitization temperature is required, thus improving the morphology of the films. For the (0001) surface, large areas of monolayer-thick graphene are formed in this way, with the size of these areas depending on the miscut of the sample. Results on the (000-1) surface are more complex. This surface graphitizes at a lower temperature than for the (0001) surface and consequently the growth is more three-dimensional. In an atmosphere of argon the morphology becomes even worse, with the surface displaying markedly inhomogeneous nucleation, an effect attributed to unintentional oxidation of the surface during graphitization. Use of a disilane environment for the (000-1) surface is found to produce improved morphology, with relatively large areas of monolayer-thick graphene.Comment: 22 pages, 11 figures, Proceedings of STEG-2 Conference; eliminated Figs. 4 and 7 from version 1, for brevity, and added Refs. 18, 29, 30, 31 together with associated discussio

Green's function method for single-particle resonant states in relativistic mean field theory

Relativistic mean field theory is formulated with the Green's function method in coordinate space to investigate the single-particle bound states and resonant states on the same footing. Taking the density of states for free particle as a reference, the energies and widths of single-particle resonant states are extracted from the density of states without any ambiguity. As an example, the energies and widths for single-neutron resonant states in $^{120}$Sn are compared with those obtained by the scattering phase-shift method, the analytic continuation in the coupling constant approach, the real stabilization method and the complex scaling method. Excellent agreements are found for the energies and widths of single-neutron resonant states.Comment: 20 pages, 7 figure

Making vortices in dipolar spinor condensates via rapid adiabatic passage

We propose to the create vortices in spin-1 condensates via magnetic dipole-dipole interaction. Starting with a polarized condensate prepared under large axial magnetic field, we show that by gradually inverting the field, population transfer among different spin states can be realized in a controlled manner. Under optimal condition, we generate a doubly quantized vortex state containing nearly all atoms in the condensate. The resulting vortex state is a direct manifestation of the dipole-dipole interaction and spin textures in spinor condensates. We also point out that the whole process can be qualitatively described by a simple rapid adiabatic passage model.Comment: 4 pages, 4 figure