Differential fates of Emiliania huxleyi-derived fatty acids and alkenones in coastal marine sediments: Effects of the benthic crustacean Palaemonetes pugio

In order to examine how benthic crustaceans affect the fates of phytoplankton-derived lipid biomarkers (fatty acids and alkenones) in coastal marine sediments, we incubated Emiliania huxleyi cells in microcosms (pre-sieved sediment cores with and without the grass shrimp Palaemonetes pugio ) over six weeks. Crustacean, transport of surface sediments, and distributions of algal lipids were followed during incubations. Crustacean activities enhanced degradation of algal fatty acids (2–4× faster) but had a small impact on algal alkenone degradation (\u3c1.4×) compared to the controls. During the first few days of incubations, alkenone concentrations were enriched while algal fatty acid concentrations were depleted in suspended particles in the overlying water of cores, indicating that P. pugio selectively grazed algal material from sediments and preferentially assimilated fatty acids over alkenones through digestion. Unlike algal fatty acids, alkenones were degraded primarily by microbial processes rather than by crustacean grazing. A substantial fraction (20–30%) of algal lipids was moved downward to the subsurface of sediments by P. pugio but algal fatty acids were more rapidly (3–6×) degraded than alkenones. In the presence of P. pugio, fatty acids bound in cell membrane and intracellular storage components degraded similarly, indicating that the crustacean activities minimized the effects of structural associations on fatty acid decomposition. Furthermore, there was no preferential degradation of 37:3 and 37:2 alkenones in both crustacean and control cores, suggesting that the U37k\u27 index (a paleotemperature indicator) was not significantly altered by P. pugio\u27s grazing or microbial decomposition

Unsteady Aerodynamic Interaction Between Rotor and Ground Obstacle

The mutual aerodynamic interaction between rotor wake and surrounding obstacles is complex, and generates high compensatory workload for pilots, degradation of the handling qualities and performance, and unsteady force on the structure of the obstacles. The interaction also affects the minimum distance between rotorcrafts and obstacles to operate safely. A vortex-based approach is then employed to investigate the complex aerodynamic interaction between rotors and ground obstacle, and identify the distance where the interaction ends, and this is also the objective of the GARTEUR AG22 working group activities. In this approach, the aerodynamic loads of the rotor blades are described through a panel method, and the unsteady behaviour of the rotor wake is modelled using a vortex particle method. The effects of the ground plane and obstacle are accounted for via a viscous boundary model. The method is then applied to a “Large” and a “Wee” rotor near the ground and obstacle, and compared with the earlier experiments carried out at the University of Glasgow. The results show that the predicted rotor induced inflow and flow field compare reasonably well with the experiments. Furthermore, at certain conditions the tip vortices are pushed up and re-injected into the rotor wake due to the effect of the obstacle resulting in a recirculation. Moreover, contrary to without the obstacle case, the peak and thickness of the radial outwash near the obstacle is smaller due to the barrier effect of the obstacle, and an up-wash is observed. Additionally, as the rotor closes to the obstacle, the rotor slipstreams impinge directly on the obstacle, and the up-wash near the obstacle is faster, indicating a stronger interaction between the rotor wake and the obstacle. Also, contrary to the case without the obstacle, the fluctuations of the rotor thrust, rolling and pitching moments are obviously strengthened. When the distance between the rotor and the obstacle is larger than 3R, the effect of the obstacle is small

Diagenesis of planktonic fatty acids and sterols in Long Island Sound sediments: Influences of a phytoplankton bloom and bottom water oxygen content

Diagenesis of organic matter in coastal sediments from Long Island Sound (LIS) was investigated by measuring fatty acids and sterols in (1) a time-series of surface sediment samples over a spring phytoplankton bloom; and (2) sediment cores collected during and after a bloom at two sites with distinctively different bottom-water oxygen contents. Time-dependent distributions of sedimentary fatty acids and sterols in LIS were strongly affected by pulsed inputs from the overlying water column, variations in benthic community, and redox-related degradation processes. The phytoplankton bloom delivered an intense pulse of unsaturated fatty acids (e.g., 16:1(ω7) and 20:5) to the surface sediments. Continuous increases of cholesterol and diunsaturated sterols after the bloom were related to zooplankton grazing processes and increase in benthic faunal abundance. High inventories of planktonic fatty acids and sterols in the upper 5 cm sediments were observed at the low oxygen site during summer, probably caused by a combination of higher input, reduced degradation rates and lower macrofaunal activity under anoxic conditions compared to oxic conditions

Developing insect resistance with fusion gene transformation of chitinase and scorpion toxin gene in maize (Zea mays L)

Transgenic plants with introduced pest-resistant genes provide an efficient alternative insect control. A binary insect-resistant gene combination, containing an insect-specific chitinase gene (chi) and a scorpion insect toxin gene (Bmk), was introduced into a maize cultivar via pollen-mediated transformation. Thirty-eight putative trans- genic plantlets with kanamycin-resistance were obtained. Transgenic statuses of plants were confirmed by South- ern blot analysis. Bioassay by inoculation of Asian corn borer (Ostrinia furnacalis Guenée; ACB) larvae indicated that the degree of ACB resistance varied among the transgenic plants. The highest average calibrated mortality of larvae was approximately 67%. The genetic analysis of T1 progeny confirmed that the inheritance of introduced genes followed the Mendelian’s rules