Impact of in-depth information and multimedia presentation on mock jurors’ comprehension of mitochondrial DNA evidence

In the courtroom, jurors are often faced with the task of evaluating complex scientific evidence. However, research suggests that jurors’ understanding of complex scientific evidence and its reliability can be prone to error. Therefore, it is important to explore how jurors’ comprehension of such evidence can be improved. The present study examined mock jurors’ (N = 162) understanding of mitochondrial DNA (mtDNA) and its reliability using a 3 (evidence presentation: basic information vs. in-depth information vs. multimedia information) x 2 (evidence strength: stronger vs. weaker) factorial design. The basic information condition was kept short and served as a baseline measure of participants’ knowledge on the subject. Participants read an expert witness testimony about mtDNA evidence in a robbery case and then answered 18 true/false questions to assess their understanding of the mtDNA evidence and its reliability. The strength of evidence did not have a statistically significant effect on jurors’ understanding of the evidence or its reliability, and no interaction effects were observed. For the presentation of evidence, by contrast, providing in-depth information on mtDNA evidence significantly improved mock jurors’ understanding of both the mtDNA evidence and its reliability. Additional illustrations presented together with the in-depth information did not further improve jurors’ scores, although exploratory post-hoc analyses suggested that the multimedia information may have facilitated jurors’ understanding of some aspects of the mtDNA evidence. Theoretical and practical implications of the findings are discussed

How much tax do the rich really pay? Evidence from the UK

Using anonymized administrative data on the population of UK taxpayers, we show that—in line with high-profile anecdotes about the tax affairs of the rich—effective average tax rates (EATRs) decline at the top of the distribution of income and capital gains. We also document substantial variation in EATRs within remuneration level: a quarter of those in the top 1 per cent pay headline rates, while another quarter pay at least 9pp less than the headline rate. Most of this effect is driven by the composition of remuneration, with investment income having lower tax rates and capital gains having lower rates still. If all individuals with income above £100,000 paid the headline rates, this would raise tax revenue on income and gains by £23 billion on a static basis, an increase of 27 per cent in the tax paid by this group

Weight loss surgery for non-morbidly obese populations with type 2 diabetes: is this an acceptable option for patients?

Aim To explore the views of non-morbidly obese people (BMI 30-40 kg/m2) with type 2 diabetes regarding: (a) the acceptability of bariatric surgery (BS) as a treatment for type 2 diabetes, and (b) willingness to participate in randomised controlled trials comparing BS versus non-surgical intervention. BACKGROUND: Despite weight management being a key therapeutic goal in type 2 diabetes, achieving and sustaining weight loss is problematic. BS is an effective treatment for people with morbid obesity and type 2 diabetes; it is less certain whether non-morbidly obese patients (BMI 30-39.9 kg/m2) with type 2 diabetes benefit from this treatment and whether this approach would be cost-effective. Before evaluating this issue by randomised trials, it is important to understand whether BS and such research are acceptable to this population. METHODS: Non-morbidly obese people with type 2 diabetes were purposively sampled from primary care and invited to participate in semi-structured interviews. Interviews explored participants' thoughts surrounding their diabetes and weight, the acceptability of BS and the willingness to participate in BS research. Data were analysed using Framework Analysi

Nurses\u27 Alumnae Association Bulletin - Volume 18 Number 1

Alumnae Notes Central Dressing Room Committee Reports Digest of Alumnae Association Meetings Graduation Awards - 1952 Greetings from Miss Childs Greetings from the President Marriages Modern Trends in Orthopaedic Surgery Necrology New Arrivals Physical Advances at Jefferson Hospital - 1953 Staff Activities - 1952-1953 Student Activities The Artificial Heart Lung Machin

Policy Brief #1: Child Care Assets: What are 14 Key Assets of Child Care Providers that Support Quality?

In 2000, university researchers at the University of Nebraska-Lincoln, Iowa State University, University of Kansas and the University of Missouri and state child care and early education program partners in four states (Missouri, Iowa, Kansas, and Nebraska) initiated the Midwest Child Care Research Consortium (MCCRC). The focus of the Consortium’s work is to conduct a multiyear study on a range of issues associated with child care quality and conditions. Across the four states, a stratified random selection of 2,022 child care providers participated in a telephone survey conducted by the Gallup Organization, representing licensed child care centers, licensed family child care homes, registered child care homes, and subsidized care license exempt family and (in one state) license exempt center care. Providers responded to questions about background and practices often associated with quality. Of the providers responding to the phone survey, 365 were randomly selected for in-depth observations to assess quality, using conventional measures of child care quality (see back of this brief). This report shows the relation between observed quality and many provider characteristics and professional improvement efforts

Nebraska Child Care Workforce and Quality: Summary Policy Brief #7

The study showed the average child care provider in Nebraska is female, married and a parent. This provider had some training or education beyond high school but not an advanced degree, was active in child care training, had a First Aid/CPR certificate, considered child care her profession or calling, had been in the child care field for over 5 years and planned to remain a provider. The average provider was observed to provide minimal quality child care. In Nebraska, using well-established observational measures of quality, center-based preschool care averaged 4.16 on the Early Childhood Environment Rating Scale (ECERS-R); 4.49 on the Infant Toddler Environment Rating Scale (ITERS); and family child care averaged 4.46 on the Family Day Care Rating Scale (FDCRS). A “5” is considered “good” quality. There was great variability across all types of care. · Family child care quality was higher in Nebraska and Missouri than in Iowa and Kansas. · In center-based care, there were no differences between providers who cared for children receiving government child care subsidies and those who did not but in family child care there were differences. Quality, training, education and professionally-oriented attitudes were lower among subsidy-receiving family child care providers than for non-subsidy receiving counterparts. · Providers in Early Head Start/Head Start partnerships offered higher quality care and received more training than other child care providers. Nebraska like two other states invested training funds to enable Early Head Start/Head Start programs to partner with programs to follow the Head Start Performance Standards and these partnerships did appear to result in higher quality than average

Delivery of temozolomide and N3-propargyl analog to brain tumors using an apoferritin nanocage

Glioblastoma multiforme (GBM) is a grade IV astrocytoma, which is the most aggressive form of brain tumor. The standard of care for this disease includes surgery, radiotherapy and temozolomide (TMZ) chemotherapy. Poor accumulation of TMZ at the tumor site, tumor resistance to drug, and dose-limiting bone marrow toxicity eventually reduce the success of this treatment. Herein, we have encapsulated >500 drug molecules of TMZ into the biocompatible protein nanocage, apoferritin (AFt), using a "nanoreactor" method (AFt-TMZ). AFt is internalized by transferrin receptor 1-mediated endocytosis and is therefore able to facilitate cancer cell uptake and enhance drug efficacy. Following encapsulation, the protein cage retained its morphological integrity and surface charge; hence, its cellular recognition and uptake are not affected by the presence of this cargo. Additional benefits of AFt include maintenance of TMZ stability at pH 5.5 and drug release under acidic pH conditions, encountered in lysosomal compartments. MTT assays revealed that the encapsulated agents displayed significantly increased antitumor activity in U373V (vector control) and, remarkably, the isogenic U373M (MGMT expressing TMZ-resistant) GBM cell lines, with GI50 values 500 molecules of the N3-propargyl imidazotetrazine analog (N3P), developed to combat TMZ resistance, and demonstrated significantly enhanced activity of AFt-N3P against GBM and colorectal carcinoma cell lines. These studies support the use of AFt as a promising nanodelivery system for targeted delivery, lysosomal drug release, and enhanced imidazotetrazine potency for treatment of GBM and wider-spectrum malignancies

Economic implications of alternative scholarly publishing models : exploring the costs and benefits. JISC EI-ASPM Project. A report to the Joint Information Systems Committee (JISC)

A knowledge economy has been defined as: “…one in which the generation and exploitation of knowledge has come to play the predominant part in the creation of wealth. It is not simply about pushing back the frontiers of knowledge; it is also about the more effective use and exploitation of all types of knowledge in all manner of economic activities” (DTI 1998). In a knowledge economy, innovation and the capacity of the system to create and disseminate the latest scientific and technical information are important determinants of prosperity (David and Foray 1995; OECD 1997). Scholarly publishing plays a key role, as it is central to the efficiency of research and to the dissemination of research findings and diffusion of scientific and technical knowledge. But, advances in information and communication technologies are disrupting traditional models of scholarly publishing, radically changing our capacity to reproduce, distribute, control, and publish information. The key question is whether there are new opportunities and new models for scholarly publishing that would better serve researchers and better communicate and disseminate research findings (OECD 2005, p14)

Comprehensive characterisation of transcriptional activity during influenza A virus infection reveals biases in cap-snatching of host RNA sequences.

Macrophages in the lung detect and respond to influenza A virus (IAV), determining the nature of the immune response. Using terminal-depth cap analysis of gene expression (CAGE), we quantified transcriptional activity of both host and pathogen over a 24-h time course of IAV infection in primary human monocyte-derived macrophages (MDMs). This method allowed us to observe heterogenous host sequences incorporated into IAV mRNA, "snatched" 5' RNA caps, and corresponding RNA sequences from host RNAs. In order to determine whether capsnatching is random or exhibits a bias, we systematically compared host sequences incorporated into viral mRNA ("snatched") against a complete survey of all background host RNA in the same cells, at the same time. Using a computational strategy designed to eliminate sources of bias due to read length, sequencing depth, and multimapping, we were able to quantify overrepresentation of host RNA features among the sequences that were snatched by IAV. We demonstrate biased snatching of numerous host RNAs, particularly small nuclear RNAs (snRNAs), and avoidance of host transcripts encoding host ribosomal proteins, which are required by IAV for replication. We then used a systems approach to describe the transcriptional landscape of the host response to IAV, observing many new features, including a failure of IAV-treated MDMs to induce feedback inhibitors of inflammation, seen in response to other treatments.IMPORTANCE Infection with influenza A virus (IAV) infection is responsible for an estimated 500,000 deaths and up to 5 million cases of severe respiratory illness each year. In this study, we looked at human primary immune cells (macrophages) infected with IAV. Our method allows us to look at both the host and the virus in parallel. We used these data to explore a process known as "cap-snatching," where IAV snatches a short nucleotide sequence from capped host RNA. This process was believed to be random. We demonstrate biased snatching of numerous host RNAs, including those associated with snRNA transcription, and avoidance of host transcripts encoding host ribosomal proteins, which are required by IAV for replication. We then describe the transcriptional landscape of the host response to IAV, observing new features, including a failure of IAV-treated MDMs to induce feedback inhibitors of inflammation, seen in response to other treatments

Modulation of the acidity of the 8-carboxamide group in the temozolomide family of antitumor imidazo[5,1-d][1,2,3,5]tetrazines

Imidazo[5,1-d][1,2,3,5]tetrazines related in structure to the anticancer drugs temozolomide and mitozolomide with modification of the 8-carboxamide group, have been synthesized, N-nitrocarboxamides by direct nitration of the corresponding carboxamides, and N-cyanomitazolomide by sodium cyanamide acylation. The NH groups in the N-nitro- and N-cyano-carboxamides were considerably more acidic than the parent carboxamide, and readily formed salts with morpholine and imidazole. X-Ray crystallography revealed that the N-nitro compound existed as such rather than the nitronic acid tautomer. Preliminary evaluation showed that enhancing the acidity of the carboxamide NH in mitozolomide analogues was detrimental to the growth inhibitory activity