3 research outputs found
Paraoxonase 1 gene polymorphisms (Q192R and L55M) are associated with coronary artery disease susceptibility in Asian Indians
Background: Coronary artery disease (CAD) is a complex
metabolic disorder in which lifestyle and genetic factors are
known to play key roles in pathogenesis. The paraoxonase 1
(PON1) enzyme has a defensive effect against CAD progression,
as it safeguards low-density lipoproteins (LDLs) from
oxidative modifications. The most extensively studied genetic
variants in the PON1 gene are Q192R and L55M, which
have been related with LDL antioxidative activity and risk of
CAD. Objective: The present case-control study intended to
examine the Q192R and L55M polymorphisms and their association
with the risk of CAD patients in north Indians.
Methods: A total of 872 subjects (412 CAD patients and 460
controls) were recruited from north India. The PON1 gene
was amplified and genotypes were studies using PCR-RFLP.
χ2 analysis was performed to compare genotype/allele frequencies
in patients and controls. Results: The present study indicated abdominal obesity, elevated body mass index, and
dyslipidemia with increased levels of total cholesterol and
triglycerides as well as reduced high-density lipoprotein
cholesterol in CAD subjects compared to healthy controls
(p < 0.05). Logistic regression analysis of the data revealed an
association of the RR genotype of the Q192R polymorphism
with an about 2-fold elevated risk of CAD (OR = 2.23, 95%
CI = 1.47–3.37, p = 0.0001). Contrariwise, the L55M polymorphism
did not show significant association with CAD (OR =
1.81, 95% CI = 0.66–4.95, p = 0.326). Conclusions: The Q192R
polymorphism in the PON1 gene may be a susceptibility
gene associated with increased risk of CAD in an Asian Indian
population
ENPP1 K121Q functional variant enhances susceptibility to insulin resistance and dyslipidemia with metabolic syndrome in Asian Indians
Background: Ectonucleotide pyrophosphatase/phosphodiesterase1
(ENPP1/PC-1) is a key modulator of the insulin signaling
pathway, and its common variant, K121Q, increases
the susceptibility to diabetes and cardiovascular diseases.
Objectives: The main objective of the present study was to
investigate the association of ENPP1 K121Q polymorphism
with the pathophysiology of metabolic syndrome (MetS) in
a north Indian population. Methods: A total of 567 participants
(303 MetS subjects and 264 healthy controls) were examined
for ENPP1 genotypes and various clinical parameters,
including body mass index (BMI), waist circumference
(WC), systolic and diastolic blood pressures (SBP/DBP), fasting
blood glucose (FBG), cholesterol, triglycerides (TG), highdensity
lipoprotein, and insulin. Genotyping was performed
using polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). Statistical analysis of the data
was done using SPSS. Results: Significant increases in BMI,
WC, SBP, DBP, FBG, TG, low-density lipoprotein, insulin, and
Homeostasis Model Assessment of insulin resistance (HOMAIR)
and of beta-cell function (HOMA-BF) were observed in
MetS patients compared to healthy controls. Logistic regression
analysis of data demonstrated a nonsignificant association
of QQ and KQ+QQ genotypes with increased risk of
MetS (OR [95% CI], 1.583 [0.455–5.507], p = 0.470 for QQ genotypes
and 1.097 [0.784–1.540], p = 0.587 for KQ+QQ genotypes).
Moreover, MetS subjects carrying Q alleles had significantly
higher levels of TG, insulin, body fat percentage,
and insulin resistance as evident by higher values of HOMAIR.
Conclusions: We conclude that ENPP1 K121Q functional
variant enhances susceptibility to insulin resistance and dyslipidemia
in MetS subjects of an Asian Indian population
Additional file 1 of Beneficial effects of GABA-producing potential probiotic Limosilactobacillus fermentum L18 of human origin on intestinal permeability and human gut microbiota
Additional file 1: Table S1. The species name and GenBank accession numbers of fecal lactobacilli isolates used in the study