Somatic embryogenesis and plant regeneration of Capsicum baccatum L.

AbstractA plant regeneration protocol via somatic embryogenesis was achieved in cotyledon and leaf explants of Capsicum baccatum, when cultured on MS medium supplemented with various concentrations of 2,4-dichlorophenoxy acetic acid (2,4-D, 0.5–5.0mgl−1) in combination with Kinetin (Kn, 0.5mgl−1) and 3% sucrose. Various stages were observed during the development of somatic embryos, including globular, heart, and torpedo-stages. Torpedo stage embryos were separated from the explants and subcultured on medium supplemented with various concentrations of different plant growth regulators for maturation. Maximum percentage (55%) of somatic embryo germination and plantlet formation was found at 1.0mgl−1 BA. Finally, about 68% of plantlets were successfully established under field conditions. The regenerated plants were morphologically normal, fertile and able to set viable seeds

Agrobacterium Mediated Genetic Transformation Of Ground Nut (Arachis Hypogaea L.) Cultivar Icgv-15311 Embryo Axis Explants For Defensin Gene Against Fungal Resistance

Genetic transformation of Ground nut (Arachis hypogaea L.) was attempted using plasmid vector pCAMBIA '3365' Present with in Agrobacterium tumifaciens strain LBA4404  with CaMV35sp (Cauliflower mosaic virus 35s RNA Promoter). A total of 323 Embryo axis, Half cut embryo, Cotyledonary nodes were employed in independent co cultivation events, of which 13 putative transgenic plants were established in Invitro conditions. The transgenic shoots obtained were initially confirmed for the introduced reporter gene by GUS histochemical assay and later by the PCR analysis. The transformed shoots were checked for the transcription of the gene by RT-PCR analysis. Southern blot analysis was carried out to confirm the stable integration of the foreign DN

Invitro Regeneration And GUS Expression Studies In Ground Nut (Arachis Hypogaea L.) Variety ICGV 15311.

Ground nut is an important source of food,edible oil,dietary vitamin, minerals, proteins, animal feed etc. In this research, an efficient of regeneration and Agrobacterium mediated genetic transformation with GUS expression is reported in a commercially important variety ICGV 15311, using embryo axis, Leaf, Half cut embryo axis, Cotyledonary node. Various combinations and concentrations of plant growth regulators are used to obtain multiple shooting and rooting.MS (Murashig and Skoog) medium supplemented with various concentrations of Auxins (IBA,NAA,IAA and 2,4D) and Cytokinins (BAP and KIN).Explants cultured on 2,4 D and KIN expressed only swelling and enlargement without further development.BAP and NAA showed more shoots on embryo axis explants on medium concentration and combination of BAP (2.0 mg/l) and NAA(0.5mg/l).Rooting obtained on IBA (2.0mg/l). The bacterial culture 1.0 OD 600, incubation with 50 µl acetosyringone and co cultivation period of 3 days were found to be efficient for transformation. Tansformed putatives grown on shooting and rooting medium and remained healthy putatives transferred to soil for hardening