Sex steroid receptors in transformed human cervical tissue.

Investigation of the binding affinity, the concentration and the DNA binding ability of the sex steroid receptors in transformed human cervical tissue has shown considerable changes in comparison to normal specimens. Lower level of hormone receptors and higher dissociation constants are the essential characteristics of the majority of analysed transformed tissue specimens. The DNA binding of steroid-receptor complexes is in agreement with mentionated data. These preliminary results are just one more evidence on the existence of relationship between modified properties of sex steroid receptors and different histological alterations of their target tissues. Further investigations are necessary to make some conclusion about the possible involvement of estrogen and progesterone receptors in pathological transformation of the portio vaginalis uteri

Promene steroidnih receptora u toku histopatoloske transformacije tkiva humanog uterusa

Estrogen (ER) and progesterone receptors (PR) were analyzed in the cytosol and nuclei prepared from specimens of human uterine tissue of patients with certain disorders identified as hyperplasia endometrii adenomatosa, myoma uteri per magnum, adenocarcinoma endometrii and adenocarcinoma corporis uteri. These investigations have revealed a different level of ER and PR in analyzed tissue specimens, as well as the existence of a relationship between changes in receptor levels and respective Kd. These changes suggest a correlation between steroid receptor levels and the type of tissue transformation. The functionality of the receptors was analyzed by the ultracentrifugation of non-activated and activated steroid-receptor complexes in sucrose density gradients, as well as by the investigation of their interaction with isolated nuclei. These results indicate that some changes in steroid receptor molecules can be detected when the tissue turns from normal to malignant transformation. On the basis of this investigation it could be proposed that the analysis of activated and non-activated steroid-receptor complexes by means of the methods used in this study can be applied as a useful clinical tool in the determination of the endocrine dependence of transformed tissues, as well as for the optimum dosing of individual treatment of patients with uterine and other tissue carcinoma