Effects of nitrogen source and effective micro-organisms on vegetative growth of shrubs of selected grapefruit (Citrus paradisi Macf) cultivars

   Grapefruit (Citrus Paradisi Macf.) is one of the most important citrus fruits in the Sudan. It can be successfully grown throughout the country where there are suitable soils and sufficient water to sustain tree growth. The objectives of this study were to find out the effects of different nitrogen sources, effective microorganisms (EM) and their combinations on the vegetative growth of shrubs of selected grapefruit cultivars. A field experiment was carried out at Umbarona, Wad Medani, Gezira State, Sudan, latitude 14.38 N, longitude 33.48 E and altitude 409 masl, in June 2012. Treatments consisted of three grapefruit cultivars: Marsh Seedless, Red Blush and Shamber. Fertilizer treatments were urea (100%), chicken manure (100%), urea with chicken manure (50% each), urea with EM, chicken manure with EM, urea (50%)+ chicken manure (50%)+ EM and unfertilized control. All fertilizer treatments were applied at the rate of 43 kg N/ha. Treatments were arranged in a split- plot design with two replicates. Grapefruit cultivars were allotted to the main plots and fertilizer treatments to the sub-plots. Data were collected on plant height (cm), stem diameter (cm), number of new branches, length of new branches (cm) and diameter of new branches (cm). Results showed that Marsh Seedless cultivar had the most vigorous vegetative growth compared to the other two cultivars. The results showed that the combination of urea, chicken manure and EM significantly gave the highest vegetative growth values, followed by chicken manure with EM, urea with chicken manure, urea with EM, urea alone, chicken manure alone and the least values of growth parameters were recorded for the unfertilized control. It is recommended to grow Marsh Seedless grapefruit cultivar and fertilize it with a combination of urea, chicken manure and EM.         يعتبر القريب فروت من أهم ثمار الحمضيات في السودان ويمكن زراعته بنجاح في جميع أنحاء البلاد حيثما توفرت التربة المناسبة والمياه الكافية لنمو الأشجار. أجريت تجربة حقلية في ام بارونة، واد مدنى ولاية الجزيرة، السودان. تهدف هذه التجربة لمعرفة تأثير مصادر النتروجين المختلفة والكائنات الحية الدقيقة الفعالة ومزجها مع بعضها البعض على النمو الخضري لشجيرات أصناف منتخبة من القريب فروت. تضمنت المعاملات ثلاثة أصناف من القريب فروت وهي مارش  ورد بلش وشامبر وسبعة معاملات أسمدة وهي  اليوريا، وزرق الدواجن، يوريا مع زرق الدواجن، يوريا مع الكائنات الحية الدقيقة الفعالة، زرق الدواجن مع الكائنات الحية الدقيقة الفعالة، يوريا مع زرق الدواجن مع الكائنات الحية الدقيقة الفعالة والشاهد. تمت إضافة الأسمدة لكل المعاملات بمعدل 43 كجم نتروجين للهكتار. صممت التجارب بطريقة القطع المنشقة بحيث كانت أصناف القريب فروت هي القطع الرئيسة ومعاملات النتروجين هي القطع الثانوية. جمعت بيانات عن طول النبات (سم) وقطر الساق (سم) وعدد الأفرع الجديدة وطول الفرع (سم) وقطر الفرع (سم). أوضحت النتائج أن الصنف مارش أعطى أعلى نمو خضري مقارنة بالصنفين رد بلش وشامبر. الخليط بين سماد اليوريا وزرق الدواجن و الكائنات الحية الدقيقة الفعالة  أعطت أعلى معدل للنمو الخضري ويليه زرق الدواجن والكائنات الحية الدقيقة الفعالة ثم اليوريا والكائنات الحية الدقيقة الفعالة ثم اليوريا وزرق الدواجن ثم زرق الدواجن لوحده ثم اليوريا لوحده وأدنى معدلات  النمو كانت في الشاهد .  يوصى بزراعة الصنف قريب فروت مارش وتسميده بخليط من اليوريا وزرق الدواجن والكائنات الحية الدقيقة الفعالة

Saudi SCD patients’ symptoms and quality of life relative to the number of ED visits

Background Individuals living with sickle cell disease (SCD) have significantly increased emergency department (ED) use compared to the general population. In Saudi Arabia, health care is free for all individuals and therefore has no bearing on increased ED visits. However, little is known about the relationship between quality of life (QoL) and frequency of acute care utilization in this patient population. Methods A cross-sectional study was conducted on 366 patients with SCD who attended the outpatient department at King Fahad Hospital, Hofuf, Saudi Arabia. Data were collected through self-administered surveys, which included: demographics, SCD-related ED visits, clinical issues, and QoL levels. We assessed the ED use by asking for the number of SCD-related ED visits within a 6-month period. Results The self-report survey of ED visits was completed by 308 SCD patients. The median number of SCD-related ED visits within a 6-month time period (IQR) was four (2-7 visits). According to the unadjusted negative binomial model, the rate of SCD-related ED visits increased by (46, 39.3, 40, and 53.5 %) for patients with fever, skin redness with itching, swelling, and blood transfusion, respectively. Poor QoL tends to increase the rate of SCD-related ED visits. Well education and poor general health positively influenced the rate of SCD-related ED visits. Well education tends to increase the rate of SCD-related ED visits by 50.2 %. The rate of SCD-related ED visits decreased by 1.4 % for every point increase in general health. Conclusion Saudi patients with sickle cell disease reported a wide range of SCD-related ED visits. It was estimated that six of 10 SCD patients had at least three ED visits within a 6-month period. Well education and poor general health resulted in an increase in the rate of SCD-related ED visits

Production of Polysaccharides (Xanthan gum) by Xanthomonas campestris pv. Sesame

Many microorganisms and plants were found produce polysaccharides which are widely varying in their composition and structure. The extracellular polysaccharide produced by   Xanthomonas campestris pv. campestris, is chemically analyzed and used industrially. However, only the xanthan gum which was ranking as the best among the ten polysaccharides was used commercially in large amounts. The xanthan gum has numerous applications in food and other industries. The present study was aiming to investigate the capacity of X; campestris pv. sesami for the production of xanthan gum, using culture media containing different carbon sources. The properties of the produced material were investigated (pH, viscosity, and chromatographic analysis using thin layer chromatography).  The results showed that the weight of the product was increasing with time reaching about 9.8g at the tenth day compared to only 2.1g on the second day. The results of the effect of different carbon sources indicated that sucrose was the best giving 9.0 g in the 7th day compared to only 1.5g by raffinose. The pH was changing from 6.7 at the beginning of the experiment to 4.2 in the tenth day. However, the viscosity of the inoculated sucrose medium was found to increase from 2.4 unit at the second day to 9.7 units after ten days. Analysis on paper chromatography showed that the produced polysaccharide contains mannose, glucuronic acid, glucose, rhamnose but no ribose nor trehalose or fructose were detected. The results indicated that the investigated bacterium was producing large amounts of xanthan gum. Although, Xanthan gum was discovered in 1950s its substantial commercial production began in 1964 and in 1969, the USA, Food and Drug Administration authorized its use in food. Xanthan gum, now has numerous uses in food and other industries. Further studies should be carried on other isolates  of the bacterium   X. campestris pv. sesami and more chemical analysis are needed

Doppler ultrasound of umbilical artery in prediction of fetal outcome in pregnancy induced hypertension Sudanese population

The pregnancy induced hypertension increase the fetal mortality and morbidity and the using of Doppler umbilical artery indices decrease the fetal mortality and morbidity however, there is few complete data about the most frequently altered Doppler US parameters to predict fetal outcome in pregnancy induced hypertension . Methods This ia cohort prospective study done in two hundred and six women of second and third trimester presenting to antenatal clinic in Soba University Hospital at the department of Obstetrics & Gynecology, in the fetus unit and critical pregnancy in the period From June 2008 to April 2013 to assess the Doppler indices of umbilical artery in pregnancy induced hypertension for prediction of prenatal outcome; 105 pregnancy induced hypertension patients and 101 women with uneventful pregnancies as normal control group included in this study . Baseline investigations and color Doppler of umbilical artery were done. Statistical analysis of data were done using SPSS, Receiver Operating Characteristic (ROC) curve analysis was performed and the area under the curve (AUC) used to determine sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) of umbilical artery Doppler indices to predict fetal outcome.Results The study determine that there was significant difference in Doppler indices in PIH and control group ( p<0.01, the mean indices of umbilical artery is higher in PIH group compared with normal pregnancy group , the mean different of S/D ratio was 0.40, mean difference of RI was 0.06 and the mean different of PI index was 0.16, high percentage of adverse fetal outcome had been reported in in Pregnancy Induced Hypertension group than in control , which was more in absent and reversed flow velocity in umbilical artery in Pregnancy Induced Hypertension compared with group of Pregnancy Induced Hypertension with present end diastolic flow velocity. Systolic/Diastolic ratio was most accurate in predicting adverse outcome in pregnancy induced hypertension patients, followed by the Pulastility index then the Resistance index (75%, 66% and 57% respectively).ConclusionThis study concluded that pregnancy induced hypertension leads to worsen placental insufficiently, which appears on the higher Doppler indices of umbilical artery to PIH patients when compared with normal pregnancy. A low diastolic flow and higher indices characterized the pregnancies with abnormal outcomes. Doppler of the umbilical artery was useful to predict fetal well being in PIH patients, high percentage of adverse fetal outcome had been reported in absent and reversed end diastolic flow velocity in umbilical artery compared with group of present flow velocity

A Survey of the Processing and Chemical Composition of Gariss Produced by Nomadic Camel Women Herders in AlGaderif State, Sudan

Abstract Gariss is the most popular stable food for the camel herders who depend on Gariss for the sustainability of their livestock. The objective of the current study is to assess the traditional fermented camel milk (Gariss) prepared by nomadic camel woman herders in AlGadarif State in Butana. It is also meant to improve the quality of camel milk products through sharing knowledge. The samples (n= 19) were collected during rainy and dry seasons. The nomad&apos;s housekeepers were interviewed in the study area. The effect of different types of containers and the additives used during Gariss preparation and the compositional quality were all estimated. The survey reported the different types of spoilage and the variations in the shelf life of Gariss. When compared with the mean values of the Gariss samples collected during dry season, the result indicated that the values of the total solids and the pH of the samples collected during the rainy season were significantly higher (p&lt;0.05), whereas the values of the fat, protein and ash were significantly lower (p&lt;0.05). The container types had a significant (p&lt;0.05) effect on the total solids, fat and ash content only. The Gariss prepared in Bukhsa showed the highest total solids content (13.15±0.54%) and that prepared in stainless steel showed the highest fat (4.65± 0.34%) content. However, when Gariss was prepared in plastic containers it showed the lowest pH value (3.59±0.16%), whereas samples from &apos;&apos;Siin&apos;&apos; (goat leather) was significantly lower (p&lt;0.05) in the ash content (0.35± 0.09%). The present study concludes that the chemical composition of Gariss from the nomadic camel women herders is affected by seasons, types of additives and containers used. Hence more studies are needed to be done on the effect of the additive and containers on Gariss quality

Tsetse Fly Infection Dynamics and its Implications with Control of Trypanosomiasis in Kajo-Keji County, South Sudan

A two-year study was conducted in Kajo-keji County South Sudan to evaluate tsetse fly infection dynamics which implicate the control of trypanosomiasis in the study area.Infection dynamics ofthe flieswas assessed and monitored using biconical traps.Captured flies were identified, segregated into sexes and age examined using wing fray and ovarian techniques for males and females, respectively. RIME-LAMP test was used to detectTrypanosome species in the midguts of wild tsetse flies.The non-parametric Wilcoxon Signed Rank Test was used to assess the difference in the number of the infected flies between the dry and the wet season counts.The mean infected G.f. fuscipes were: male 6 ±1.4, female 3 ± 1.0 and male 10 ± 2.0, female 3 ± 0.7 in the dry and wet seasons of the year 2011; male,6 ± 1.4,female 6 ±1.4 and male,10 ± 2, female3 ± 0.8 in the dry and wet seasons of 2012.Infection showed significant differences (Z=-2.03,  P = 0.04) with both seasons in 2011 and no significant differences (Z=-1.41, P = 0.16) in 2012. Number of infected male and female flies was positively correlated with the fly age in the dry (Male, R2=0.94; female, R2=0.86) and wet (Male, R2=0.97; female, R2=1) seasons in 2011 and (Male, R2=0.90; female, R2=0.94) in the dry and wet (Male, R2= 0.97 female, R2=1) seasons in 2012.These results showed thatG. f.fuscipeswere  infected with T.brucei gambiense and  they were proved  to be potential vectors for HAT in the study area. Hence, the implications of Tsetse fly infection dynamics in the control of trypanosomiasis need development of further control strategies for sustainable development of livestock and human resources in Kajo-keji County.

Characterization of the inclusion complex of zerumbone with hydroxypropyl--cyclodextrin

In this paper we investigated the inclusion complexation between zerumbone (ZER) and hydroxylpropyl- -cyclodextrin (HPCD) at four different temperatures: 293–318 ◦K. The thermodynamic parameters (H, S and G) for the formation of the complex were obtained from the van’t Hoff equation. The complex with HPCD was characterized by differential scanning calorimetry (DSC), X-ray diffractometry (XRD), Fourier transform infrared spectroscopy (FT-IR), and molecular modeling using PM6. The solubility of ZER was enhanced >30 fold after complexation. Calculations show that ZER penetrates completely into the cavity of HPCD. The complex retained its cytotoxic activity as shown by in vitro cell survival assay on human cervical cancer (Hela), breast cancer (MCF7 and MDA-MB 231) and human leukemic (CEMss) cell lines. HPCD is, therefore, a suitable encapsular capable of forming thermodynamically stable complex with ZER for save delivery of the compound as an anticancer drug in the future

Liquid chromatography - tandem mass spectroscopic method for the determination Of zerumbone in human plasma and its application to Pharmacokinetics

A rapid, sensitive, specific and selective LC-MS/MS method for the determination of zerumbone (ZER) in human plasma using 2,4-diamino-6-(4-methoxyphenyl)-1,3,5-triazine (DMTZ) as an internal standard (IS) has been developed and validated. ZER was chromatographed on C8 column using a mobile phase of acetonitrile/water (80:20, v/v) at a flow rate of 0.25 ml min(-1) . Quantitation was achieved using ESI+ interface, employing multiple reaction monitoring (MRM) mode at m/z 219 > 81 and 218 > 134 for ZER and IS, respectively. The calibration standards were linear over a range of 5-3000 ng ml(-1) (r(2)=0.9994) with an LLOQ of 5 ng ml(-1) (RSD %; 11.4% and bias%; 9.5%). Intra- and inter-day precision of ZER assay ranged from 0.18 to 3.56% with accuracy (bias) that varied between -5.09 and 4.3%, demonstrating good precision and accuracy. Recoveries of ZER and the IS from human plasma were above 85%. The developed method was validated for the determination of ZER in rat plasma. Linearity, stability of ZER and the ME on rat plasma were discussed. The applicability of the developed method was demonstrated by measuring ZER in rat plasma samples following intravenous and intraperitoneal administration of ZER prepared in hydroxypropyl-β-cyclodextrin (HPβCD) and sodium carboxymethyl cellulose (CMC), respectively, in 20 mg kg(-1) and this study indicated a clear significant difference (p<0.05) in pharmacokinetic parameters of ZER in ZER/HPβCD complex compared with ZER in CMC preparation

Screening of some sorghum genotypes for resistance to sorghum midge, Stenodiplosis (= Contarinia) sorghicola Coqillet (diptera: Cecidomyiidae) under gedarif rainfed conditions

Preliminary experiments were conducted at Northern area, Gedarif State during seasons of 2002-03, 2006-07.Where as advanced trials during 2008/2009 to 2012/2013 at northern and Southern areas. The objective was to evaluate selected sorghum genotypes for sorghum midge resistance. A total of 3000 accessions were obtained from Gene Bank Resources. Resistant genotype, DJ 6514 (Resistant Check already released in 2007) was obtained from International Crops Research Institute for the Semi-Arid Tropics (ICRISAT). Result showed that the midge damage rating was significantly different among genotypes. The midge damage rating scores ranged between, 1.3- 8.6; 1.1- 9.2; 1.4- 9.0; 1.1 – 9.4; and 1.2- 9.2 for all seasons (2008/09; 2009/10; 2010/11; 2011/12 and 2012/13). However, the lowest midge damage rating was recorded by DJ 6514 (Resistant check), followed by P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; and Safra (1.4; 1.5; 1.5, 1.6 and 1.7), respectively. Genotypes, P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Wad Baco; Safra and Harerai showed lowest % yield loss and performed similar to the resistant check (14.2; 14.5; 14.5; 17.0 and 17.3%), respectively. The combined analysis showed that a significant difference was observed between genotypes. The genotypes were significantly different in panicle types, compact and semi-compact headed genotypes showed lower % glumes coverage (1.3- 4.5%), while semi-compact headed genotypes ranged between 5.5 – 7.8%. Genotypes, Wad Baco; P₁ 570162 (Hag Abbakar); GBM 30 (Early Feterita); Safra; Wad Ahmed; Harerai and Wad Akar showed the shortest glumes coverage (1.3; 1.3; 1.5; 1.5; 1.6; 1.7 and 1.8 %). Compact headed genotypes recorded lowest midge density (6.5 adults/ 5 heads), while the semi-compact headed genotypes were recorded 15.7 adults/ 5 heads compared with others types of heads

Health-related quality of life in patients with sickle cell disease in Saudi Arabia

Background There is a lack of research concerning health-related quality of life (HRQoL) in Saudi patients with sickle cell disease (SCD), particularly among adult populations. The aim of the current study was to describe the characteristics of SCD patients and their impact on their quality of life (QoL). Methods Six hundred twenty-nine adult SCD patients who attended King Fahad Hospital in Hofuf and King Fahad Central Hospital in Jazan were included in the analysis. Demographic/clinical data were collected and an Arabic version of the Medical Outcomes 36-Item Short-Form Health Survey (SF-36) questionnaire was used to assess QoL. Results SCD patients who hold a university degree reported positive impacts on the following domains of SF-36: physical role function, vitality, emotional well being, social function, pain reduction, and general health (P = .002, P = .001, P = .001, P = .003, P = .004, and P = .001, respectively). In general, patients with fever, skin redness, swelling, or history of blood transfusion tended to impair the health status of the SF-36. A multivariate analysis revealed that patients with a university degree tended to report high scores of physical role functions, emotional role function, and vitality. Patients with regular exercise tend to increase vitality, social function, general health, and reduce pain. Unemployment tends to lessen vitality and worsen pain. On average, pain, social function, and physical function scores tended to worsen in patients with swelling or history of blood transfusion. Conclusions This study highlighted that poor education, fever, skin redness, and swelling were negatively associated with specific components of SF-36. SCD patients with a history of blood transfusion found their QoL poorer, whereas regular exercise tended to improve QoL