Cytotoxicity test of binjai leaf (Mangifera caesia) ethanol extract in relation to Vero cells

Background: Binjai leaves (Mangifera caesia) constitute one part of a medicinal plant from South Borneo that contains potential anticancer and antioxidant flavonoids. Before using medicinal plants as adjuvant therapy material, a cytotoxicity test of a material extract needs to be conducted in order to establish the safety of natural ingredients that will be used in the production of medicinal products. Purpose: This research aimed to determine whether the ethanol extract of binjai leaves proved cytotoxic to Vero cells and determine the value of IC50 after the administering of ethanol extract of Binjai leaves by means of an MTT assay method. Methods: This research incorporated a true experimental method with posttest-only control design that consisted of ten groups. The Binjai leaf ethanol extract of varying concentrations was administered to eight groups, namely;1.25µg/mL, 62.5µg/mL, 125µg/mL, 250µg/mL, 500µg/mL, 1000µg/mL, 2000µg/mL and 4000µg/mL. The control groups consisted of two groups, one cell control group and one media control group. The cell viability percentage was calculated by an absorbent of ELISA reader. Results: The probit analysis result had an IC50 value of 2498.48µg/mL (IC50>1000µg/mL constituted a non-toxic category). Conclusion: Ethanol extract of Binjai leaves is not cytotoxic to Vero cells as shown by an assay MTT method which produced an IC50 value of 2498.48µg/mL

Differences in Panoramic Mandibular Index value between male and female hypertension patients using panoramic radiography

Objectives: This study is aimed to analyze the panoramic mandibular index value in male and female hypertensive patients using panoramic radiography. Materials and Methods: This research is a comparative analytic research with a purposive sampling technique. The study sample was hypertensive patients at Ulin Hospital, Banjarmasin from March to May 2023. Results: The results showed that there was no statistically significant difference through statistical data on the panoramic mandibular index value between men (0.27 ± 0.07) and women (0.26 ± 0.09), The age group that experienced a decrease in mandibular cortical thickness was most in the age group ≥ 65 years (0.22 ± 0.06). Conclusion: It is known that there is no significant difference in the panoramic mandibular index value between women and men

Differences of Gonial Index value in male and female hypertension patients using panoramic radiography

Objectives: This study is aimed to measure the Gonial index (GI) value in male and female hypertensive patients using panoramic radiography. Materials and Methods: This is a comparative analytical research. The sampling technique used was the purposive sampling technique. The sample of this study was primary data from panoramic radiographs of hypertensive patients who had been X-rayed at the Radiology Installation of Ulin General Hospital, Banjarmasin. Results: There was no significant difference in the gonial index (GI) values between men (17.83 mm) and women (19.17 mm), with the size of the right region of the female 20.33 mm and the left of the female 16.67 mm and the size of the right region of the male is 17.03 mm and the left male is 19.97 mm. Conclusion: There was no significant difference between the gonial index (GI) values ​​between women and men, although the decrease of  bone density in women is higher than in men

The potentiation of Mangifera casturi bark extract on interleukin-1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Background: The main oral health problem in Indonesia is the high number of tooth decay. Tooth extraction is the treatment often received by patients who experience tooth decay and the wound caused by alveolar bone resorption. Bark of Mangifera casturi has been studied and proven to contain secondary metabolite which has the ability to increase osteoblast’s activity and suppress osteoclast’s activity. Purpose: The purpose of this study was to analyze interleukin-1 beta (IL-1β) and bone morphogenic protein-2 (BMP-2) activities during bone remodeling after Mangifera casturi’s bark extract treatment. Method: This study was laboratory experimental research with randomized post-test only control group design. The Mangifera casturi bark was extracted using 96% ethanol maceration and n-hexane fractionation. This study used 40 male Wistar rats which are divided into 4 groups and the tooth extraction was performed on the rats’ right mandible incisive tooth. The four groups consisted of 6.35%, 12.7%, 25.4% extract treatment group, and a control group. Wistar’s mandibles were decapitated on the 7th and 14th day after extraction. Antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done using immunohistochemistry. Result: There was a significant difference of IL-1β and BMP-2 expressions in 6,35%, 12,7%, and 25,4% treatment groups compared to control group with p<0.05. Conclusion: Mangifera casturi’s bark extract was able to suppress the IL-1β expression and increase the BMP-2 expression during bone remodeling after tooth extraction

POTENSI FRAKSI KULIT BATANG Mangifera casturi 12,7% TERHADAP EKSPRESI IL-1β DAN BMP-2 SELAMA REMODELING TULANG SETELAH PENCABUTAN GIGI

The main problem of Indonesian oral health is the high number of tooth decay. Tooth extraction is an action which is often experienced by patient who have tooth decay and the wound caused alveolar bone resorption. Bark of Mangifera casturi has been researched containing secondary metabolite which has ability to increase osteoblast’s activity and suppress osteoclast’s activity. Objective: The purpose of this study analyzed the IL-1β and BMP-2 activity during the bone remodeling after Mangifera casturi’s bark fraction treatment. Methods: This study used 40 male Wistar rats which are divided in 4 group. The Mangifera casturi bark extraction was done by 96% ethanol maceration and nhexane fractionation. The extraction was done on mandible incisive tooth. The research material in each treatment group a Mangifera casturi (Kosterm.) gel extract dose of 6,35%, 12,7%, and 25,4%. Wistar’s mandible were decapitated at interval 7 and 14 days after extraction. The antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done by immunohistochemistry

The potentiation of Mangifera casturi bark extract on interleukin- 1β and bone morphogenic protein-2 expressions during bone remodeling after tooth extraction

Background: The main oral health problem in Indonesia is the high number of tooth decay. Tooth extraction is the treatment often received by patients who experience tooth decay and the wound caused by alveolar bone resorption. Bark of Mangifera casturi has been studied and proven to contain secondary metabolite which has the ability to increase osteoblast’s activity and suppress osteoclast’s activity. Purpose: The purpose of this study was to analyze interleukin-1 beta (IL-1β) and bone morphogenic protein-2 (BMP-2) activities during bone remodeling after Mangifera casturi’s bark extract treatment. Method: This study was laboratory experimental research with randomized post-test only control group design. The Mangifera casturi bark was extracted using 96% ethanol maceration and n-hexane fractionation. This study used 40 male Wistar rats which are divided into 4 groups and the tooth extraction was performed on the rats’ right mandible incisive tooth. The four groups consisted of 6.35%, 12.7%, 25.4% extract treatment group, and a control group. Wistar’s mandibles were decapitated on the 7th and 14th day after extraction. Antibody staining on preparations for the examination of IL-1β and BMP-2 expressions was done using immunohistochemistry. Result: There was a significant difference of IL-1β and BMP-2 expressions in 6,35%, 12,7%, and 25,4% treatment groups compared to control group with p<0.05. Conclusion: Mangifera casturi’s bark extract was able to suppress the IL-1β expression and increase the BMP-2 expression during bone remodeling after tooth extraction