2 research outputs found
Use of Dimethyl Pimelimidate with Microfluidic System for Nucleic Acids Extraction without Electricity
The isolation of
nucleic acids in the lab on a chip is crucial
to achieve the maximal effectiveness of point-of-care testing for
detection in clinical applications. Here, we report on the use of
a simple and versatile single-channel microfluidic platform that combines
dimethyl pimelimidate (DMP) for nucleic acids (both RNA and DNA) extraction
without electricity using a thin-film system. The system is based
on the adaption of DMP into nonchaotropic-based nucleic acids and
the capture of reagents into a low-cost thin-film platform for use
as a microfluidic total analysis system, which can be utilized for
sample processing in clinical diagnostics. Moreover, we assessed the
use of the DMP system for the extraction of nucleic acids from various
samples, including mammalian cells, bacterial cells, and viruses from
human disease, and we also confirmed that the quality and quantity
of the nucleic acids extracted were sufficient to allow for the robust
detection of biomarkers and/or pathogens in downstream analysis. Furthermore,
this DMP system does not require any instruments and electricity,
and has improved time efficiency, portability, and affordability.
Thus, we believe that the DMP system may change the paradigm of sample
processing in clinical diagnostics