Rapid multi sample DNA amplification using rotary-linear polymerase chain reaction device (PCRDisc)

Multiple sample DNA amplification was done by using a novel rotary-linear motion polymerase chain reaction (PCR) device. A simple compact disc was used to create the stationary sample chambers which are individually temperature controlled. The PCR was performed by shuttling the samples to different temperature zones by using a combined rotary-linear movement of the disc. The device was successfully used to amplify up to 12 samples in less than 30 min with a sample volume of 5 μl. A simple spring loaded heater mechanism was introduced to enable good thermal contact between the samples and the heaters. Each of the heater temperatures are controlled by using a simple proportional–integral–derivative pulse width modulation control system. The results show a good improvement in the amplification rate and duration of the samples. The reagent volume used was reduced to nearly 25% of that used in conventional method

Estimating social distance in public places for COVID-19 protocol using region CNN

The Coronavirus disease has spread throughout the world and its fear has made people to be more cautious in public places. Since precautionary measures are the only reliable protocol to defend ourselves, social distancing is the only best approach to defend against the pandemic situation. The reproduction number i.e. R0 factor of COVID-19, can be slowed down only through the physical distancing norms. This research proposes a deep learning approach for maintaining the social distance by tracking and detecting the people present indoor and outdoor scenarios. Surveillance video is taken as the input and applied into you only look once (YOLO) V3 algorithm. The persons in the video are identified based on the segmentation algorithm present within the framework and then using Euclidean distance the image is evaluated. The bounding box algorithm helps to segregate the humans based on the minimum distance threshold. The proposed method is evaluated for images with peoples in the market, availing essential commodities and students entry inside a campus. Our proposed region-based convolutional neural network (RCNN) algorithm gives a better accuracy over the traditional models and hence the service can be implemented in general for places where social distancing is mandatory

Monitoring of the Social Distance between Passengers in Real-time through Video Analytics and Deep Learning in Railway Stations for Developing the Highest Efficiency

Near the end of December 2019, the globe was hit with a major crisis, which is nothing but the coronavirusbased pandemic. The authorities at the train station should also keep in mind the need to limit the spread of the covid virus in the event of a global pandemic. When it comes to controlling the COVID-19 epidemic, public transportation facilities like train stations play a pivotal role because of the proximity of so many people who may be exposed to the virus. Using common place CCTV cameras and deep learning with simple online and real-time (DeepSORT) methods, this study develops social distance monitoring using a YOLOv4 identification of a Surveillance Object Model. Based on experiments conducted with a minicomputer equipped with an Intel 11th Gen Intel(R) Core(TM) i3-1115G4 at 3.00GHz, 2995 Mhz, two Core(s), four Logical processor, four gigabytes of random-access memory (RAM), this paper makes use of CCTV surveillance, which was put into practice at the Guindy railway station, Chennai, Tamilnadu in India in order to detect the violation of social distancing

Optimal knowledge extraction technique based on hybridisation of improved artificial bee colony algorithm and cuckoo search algorithm

We present a framework that we are currently developing, that allows one to extract knowledge from the knowledge discovery in database (KDD) dataset. Data mining is a very active and space growing research area. Knowledge discovery in databases (KDD) is very useful in scientific domains. In simple terms, association rule mining is one of the most well-known methods for such knowledge discovery. Initially, database are divided into training and testing for the aid of fuzzy generating the rules using fuzzy rules generation the set of rules are generated from the given dataset. From the generated rules, we are extracting the significant rules by using the improved artificial bee colony algorithm and cuckoo search algorithm (IABCCS). After extracting optimal knowledge from the dataset via rules, the data will be classified using fuzzy classifier with the aid of this finally we will classify the attack and normal

A technique to stock market prediction using fuzzy clustering and artificial neural networks

Stock market prediction is essential and of great interest because success- ful prediction of stock prices may promise smart benets. These tasks are highly complicated and very dicult. Many researchers have made valiant attempts in data mining to devise an ecient system for stock market movement analysis. In this paper, we have developed an ecient approach to stock market prediction by employing fuzzy C-means clustering and articial neural network. This research has been encouraged by the need of predicting the stock market to facilitate the investors about buy and hold strategy and to make prot. Firstly, the original stock market data are converted into interpreted historical (nancial) data i.e. via technical indi-cators. Based on these technical indicators, datasets that are required for analysis are created. Subsequently, fuzzy-clustering technique is used to generate dierent training subsets. Subsequently, based on dierent training subsets, dierent ANN models are trained to formulate dierent base models. Finally, a meta-learner, fuzzy system module, is employed to predict the stock price. The results for the stock market prediction are validated through evaluation metrics, namely mean absolute deviation, mean square error, root mean square error, mean absolute percentage error used to estimate the forecasting accuracy in the stock market. Comparative analysis is carried out for single Neural Network (NN) and existing technique neu- ral. The obtained results show that the proposed approach produces better results than the other techniques in terms of accuracy

Estimating social distance in public places for COVID-19 protocol using region CNN

The coronavirus disease has spread throughout the world and its fear has made people to be more cautious in public places. Since precautionary measures are the only reliable protocol to defend ourselves, social distancing is the only best approach to defend against the pandemic situation. The reproduction number i.e. R0 factor of COVID-19, can be slowed down only through the physical distancing norms. This research proposes a deep learning approach for maintaining the social distance by tracking and detecting the people present indoor and outdoor scenarios. Surveillance video is taken as the input and applied into you only look once (YOLO) V3 algorithm. The persons in the video are identified based on the segmentation algorithm present within the framework and then using Euclidean distance the image is evaluated. The bounding box algorithm helps to segregate the humans based on the minimum distance threshold. The proposed method is evaluated for images with peoples in the market, availing essential commodities and students entry inside a campus. Our proposed region-based convolutional neural network (RCNN) algorithm gives a better accuracy over the traditional models and hence the service can be implemented in general for places where social distancing is mandatory

User Activity Analysis Via Network Traffic Using DNN and Optimized Federated Learning based Privacy Preserving Method in Mobile Wireless Networks

Mobile and wireless networking infrastructures are facing unprecedented loads due to increasing apps and services on mobiles. Hence, 5G systems have been developed to maximise mobile user experiences as they can accommodate large volumes of traffics with extractions of fine-grained data while offering flexible network resource controls. Potential solutions for managing networks and their security using network traffic are based on UAA (User Activity Analysis). DLTs (Deep Learning Techniques) have been recently used in network traffic analysis for better performances. These previously suggested techniques for network traffic analysis typically need voluminous information on network usages. Hence, this work proposes OFedeMWOUAA (optimal federated learning-based UAA technique with Meadow Wolf Optimisation) and DNN (deep Neuron Networks) for minimizing risks of data leakages in MWNs (Mobile Wireless Networks). In the proposed OFedeMWOUAA, the need to submit data to cloud servers does not arise because it trains DLTs locally and only uploads model gradients or knowledge weights. The OFedeMWOUAA approach effectively decreases dangers to data privacies with very minor performance losses in simulations

Estimation of Social Distance for COVID19 Prevention using K-Nearest Neighbor Algorithm through deep learning

Coronavirus disease has a crisis with high spread throughout the world during the COVID19 pandemic period. This disease can be easily spread to a group of people and increase the spread. Since it is a worldly disease and not plenty of vaccines available, social distancing is the only best approach to defend against the pandemic situation. All the affected countries' governments declared locked-down to implement social distancing. This social separation and persons not being in a mass group can slow down the spread of COVID19. It reduces the physical contact between infected persons and normal healthy persons. Almost every health organization tells that to follow social distancing people should maintain at least 6 feet of distance from each other. This research proposes a deep learning approach for social distancing which is developed for tracking and detecting people who are in indoor as well as outdoor scenarios using YOLO V3 video analytic technique. This approach focuses to inspect whether the people are maintaining social distancing in many areas, using surveillance video with measuring the distance in real-time performance. Most of the early studies of detecting social distance monitoring were based on GPS for tracking the movements of people where the signals could be lost. On the other hand, some countries use drones to detect large gatherings of people who cannot have a clear view at night times [10]. In the future, the proposed system can be used fully for detecting threats in the public crowded or it can detect any person affected by critical situations (ie fainting, Cordia arrest) or planting the crops in the forms evenly with a uniform measurement. This proposal could be used in many fields like crowd analysis, autonomous vehicles, and human action recognition and could help the government authorities to redesign the public place layout and take precautionary action in the risk zones. This system analyses the social distancing of people by calculating the distance between people to slow downing the spread of the COVID 19 virus