The E-box DNA binding protein Sgc1p suppresses the gcr2 mutation, which is involved in transcriptional activation of glycolytic genes in Saccharomyces cerevisiae

AbstractGlycolytic gene expression is mediated by the Gcr1p-Gcr2p transcriptional activation complex. A screen for multicopy suppressors of gcr2 yielded SGC1. SGC1’s suppression activity was specific to gcr2, it did not extend to gcr1. Disruption of SGC1 moderately affected glycolytic enzyme activities, although no growth defect was evident. Sgc1p exhibits a bHLH motif which is characteristic of E-box DNA-binding proteins. DNA footprinting experiments demonstrated Sgc1p’s ability to bind at an E-box. However, its binding specificity was less than 10-fold, which is also characteristic of E-box binding proteins. LexA fusion experiments demonstrated that Sgc1p has weak intrinsic activating activity independent of GCR1 and GCR2. We propose that Sgc1p binds at E-boxes of glycolytic genes and contributes to their activation

Reduction of Postoperative Pain by Addition of Intravenous Acetaminophen after Total Hip Arthroplasty: A Retrospective Cohort Study

We evaluated the analgesic effects of multimodal pain control in which intravenous acetaminophen (IV APAP) was added to the standard protocol for Japanese patients who had undergone a total hip arthroplasty (THA). We performed a retrospective cohort study of 180 patients aged 66.4±10.5 years (30% male) who had undergone a THA (Oct. 2014 to Feb. 2015) at our hospital. The control patients were administered the standard analgesic protocol: flurbiprofen axetil as a continuous intravenous infusion and oral celecoxib (NAPAP; n=109). The patients in the new analgesic protocol group received IV APAP in addition to the standard analgesic protocol (APAP; n=71). The primary outcome was the maximum value of postoperative pain the patients reported on a numerical rating scale (NRS) during the first 24 h post-surgery. A univariate analysis and multivariate analyses adjusted for age, sex, the stage of hip osteoarthritis, preoperative pain, and surgical time showed that the maximum postoperative pain NRS scores during the first 24 h after surgery was significantly lower when the APAP protocol was used. The addition of IV APAP to the current standard multimodal analgesia protocol for Japanese patients who have undergone a THA may decrease the patients’ postoperative pain

Usefulness of Cardiac Computed Tomography in the Diagnosis of Prosthetic Coronary Artery Graft with Interposition Procedure

An 80-year-old Japanese man was admitted with orthopnea and pitting edema of both lower legs. We diagnosed congestive heart failure (CHF) on the basis of a chest X-ray and an echocardiogram. An electrocardiogram showed a heart rate of 120 beats/min with atrial fibrillation rhythm (Af). The patient developed aortic valve failure and destruction of the base of right coronary artery (RCA) due to infectious endocarditis at 71 years of age. The patient underwent aortic valve replacement and coronary artery bypass grafting with an interposed graft with polyester vascular graft to RCA. The patient recovered from CHF after the 6 days of treatment with diuretics and verapamil. We confirmed the patency of coronary arteries and bypass grafts using a 64-slice cardiac computed tomography scan (CT) and diagnosed CHF due to Af. Here we describe the estimation of the prosthetic coronary artery graft patency with the interposition procedure using 64-slice cardiac CT

Synthesis, characterization, and photovoltaic applications of dithienogermole-dithienylbenzothiadiazole and -dithienylthiazolothiazole copolymers

New dithienogermole-based conjugated polymers were synthesized by the Stille coupling reactions of distannyldithienogermole and dibromoarene, and their photovoltaic properties were studied. These polymers possess low band gaps with broad absorptions covering the 400-800 nm range, and exhibit good film forming properties. Bulk hetero-junction solar cells prepared from blends of these polymers with PC(70)BM exhibit high power conversion efficiency up to 2.38%

RELATIONSHIP BETWEEN PROGRESSION OF AORTIC STENOSIS AND INFLAMMATORY CHANGE IN AORTIC VALVE IN HEMODIALYSIS PATIENTS

The entire manuscript is available for download as a single PDF file. Higher-resolution images are unavailable. For assistance, please contact [email protected]. Fieldwork Team: Philippe Beaujard (Director of Research, French National Centre for Scientific Research). Technical Team: Dr. Vika Zafrin (Digital Scholarship Librarian, BU Libraries), Eleni Castro (OpenBU and Electronic Theses & Dissertations Librarian, BU Libraries), Dr. Fallou Ngom (Director of the African Studies Center), Dr. Peter Quella (Assistant Director, African Studies Center), Mustapha Hashim Kurfi (PhD Candidate, Department of Political Science), and Zachary Gersten (Research Assistant, African Studies Center). This collection of Malagasy Ajami materials is copied as part of the African Studies Center’s African Ajami Library. This project is partly funded by the BU African Studies Center. We thank Dr. Tim Longman, past Director of the African Studies Center, and the entire African Studies team for their support. For Inquiries: Please contact Professor Fallou Ngom ([email protected]).The material is the second part of the sixth of eleven texts (the fourth text and the second and third parts of the eleventh were not digitized) owned by Iaban’i Totôry, a diviner-healer (called ombiasy in Malagasy). Iaban’i Totôry belonged to the Anakara Clan and lived in a village called Vatomasina in the Antemoro region (in the valley of the Matatàña River). He was known to be the grandson of a famous religious chief in his village, and was close with the French colonial administration in his region, with whom he also shared the material. The original author of the material is unknown. The material was photographed between 1983 and 1990. The pages were made out of a local plant called harandrànto in Malagasy, likely of the genus Afzelia. The material was bound in zebu skin and sinew. While the exact content of material is unknown, it is believed to contain guidance for charms, divination, and healing through prayers, geomancy, and astrology

Public subsidies and the recommendation of child vaccines among primary care physicians : a nationwide cross-sectional study in Japan.

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