Aspectos radiográficos e densitométricos de fraturas experimentais do radio de cães tratadas com plasma rico em plaquetas

The present article aimed to assess bone healing of experimental radial fractures, treated or not with autologous PRP, by means of radiographic and densitometric studies. Eleven dogs were randomly allocated in two experimental groups: control group (CG n=6) PRP group (PRPG n=5). All dogs underwent radial osteotomy and osteosynthese (external skeletal fixation) of the right forelimb, in which a 2.0 mm defect was created. The gap was filled PRP in the treated group (PRPG); wile CG received no treatment. Radiographic and densitometric studies were performed immediately at the end the procedure and 14, 21, 28, 35, 45 and 60 days after the surgery (M0, M14, M21, M28, M35, M45 e M60). Way repeated measures ANOVA with Tukey as post hock test; Friedman test and Dunnís post hock test were used to compare data in different moments; and Mann-whitney test in different groups (p < 0.05). About assessment to moments; radiographc scores and densitometric values changed significantly at moment 0 and 60 within the PRPG. Comparing groups radiographic scores changed significantly from M28 and densitometric values from M45 and M60. Concluded under the conditions of this study; that PRP can be used as an adjuvant therapy to improve bone healing of experimental radial fractures (gap 2.0 mm) of dogs treated with external skeletal fixation.O presente artigo teve como objetivo avaliar a cicatrizaÁ„o Ûssea de fraturas experimentais do radio de c„es, tratadas ou n„o com o PRP autÛgeno, por meio de estudos radiogr·fico e densitomÈtrico. Foram utilizados 11 c„es, alocados aleatoriamente em dois grupos experimentais: o grupo controle (G-controle, n=6) e o grupo PRP (G-PRP, n=5). Todos os animais foram submetidos ‡ osteotomia e osteossÌntese (fixador esquelÈtico externo) do r·dio direito, gerando-se um gap de 2mm, que foi preenchido com PRP apenas no grupo G-PRP. Os estudos radiogr·ficos e densitomÈtricos foram realizados no pÛs-operatÛrio imediato e aos 14, 21, 28, 35, 45 e 60 dias de pÛs-operatÛrio (M0, M14, M21, M28, M35, M45 e M60). A an·lise estatÌstica foi realizada por meio da An·lise de Vari‚ncia com Medidas repetidas (ANOVA) e teste de Tukey para comparaÁ„o de mÈdias; teste de Friedman seguido do teste de Dunn para comparar os momentos em cada grupo e o teste de Mann-whitney para comparar os grupos em cada momento (p<0,05). Referente ‡ avaliaÁ„o entre os momentos houve diferenÁa significativa entre M0 e M60 nas avaliaÁıes radiogr·ficas e densitomÈtricas dentro do grupo G-PRP. Na comparaÁ„o entre os dois grupos houve diferenÁa estatÌstica na avaliaÁ„o radiogr·fica a partir de M28; e na avaliaÁ„o densitomÈtrica nos momentos M45 e M60. Conclui-se, nas condiÁıes deste estudo, que o PRP pode ser utilizado como terapia adjuvante, pois promove cicatrizaÁ„o Ûssea precoce em fraturas experimentais (gap de 2,0mm) do radio de c„es tratadas com fixador esquelÈtico externo.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Diabetic Rats Present High Mean Platelet Count in the Presence of Oral Infections

<div><p>Abstract Platelet count is associated with inflammatory diseases like diabetes mellitus (DM), which in turn, is related in a bidirectional manner with apical periodontitis and periodontal disease. The aim of this study was to evaluate the effects of apical periodontitis and/or periodontal disease on mean platelet count in a rat model of diabetes mellitus. Eighty Wistar rats were randomly divided into 8 groups (n=10): control (C), apical periodontitis (AP), periodontal disease (PD), apical periodontitis with periodontal disease (AP-PD), diabetes mellitus (DM), diabetes mellitus with apical periodontitis (DM-AP), diabetes mellitus with periodontal disease (DM-PD) and diabetes mellitus with apical periodontitis and periodontal disease (DM-AP-PD). Rats were anesthetized and DM was induced with a single dose of streptozotocin diluted in citrate buffer solution. After 6 days, the DM was confirmed. The animals were sedated and apical periodontitis was induced by dental exposure and periodontal disease was induced by periodontal ligature. After 30 days, animals were anesthetized and the blood was collected by cardiac puncture. Samples were processed and the mean platelet count was obtained. Data were tabulated and subjected to statistical analysis (p<0.05). Diabetic rats had higher mean glycemic levels compared with nondiabetic rats at 6 and 36 days after DM induction (p<0.05). The DM-PD and DM-PD-AP groups showed increased mean platelet count compared to control and AP groups (p<0.05). The periodontal disease alone or associated with apical periodontitis influence mean platelet count in a rat model of diabetes mellitus.</p></div