3 research outputs found
Multispectroscopic Investigations of the Binding Interaction between Polyethylene Microplastics and Human Hemoglobin
In
this investigation, different multispectroscopic analytical
techniques have been used to explore the interaction between polyethylene
microplastics (PE-MPs) and human hemoglobin (HHb), an oxygen carrier
in the human blood circulatory system. Ultraviolet–visible
absorption studies have demonstrated that HHb molecules may interact
with PE-MPs, and thermal melting studies have indicated that PE-MPs
have a stabilizing effect on HHb. Further circular dichroism and Fourier
transform infrared spectroscopic studies have revealed the distinct
changes in HHb’s secondary structures caused by the formation
of the HHb–PE-MP binding complex. These findings imply that
PE-MPs could enter the blood circulation system of humans and may
be hazardous to humans. This work explains the potential binding interaction
of microplastics at the molecular level and offers insight into the
intermolecular interaction between PE-MPs and HHb
Multispectroscopic Investigations of the Binding Interaction between Polyethylene Microplastics and Human Hemoglobin
In
this investigation, different multispectroscopic analytical
techniques have been used to explore the interaction between polyethylene
microplastics (PE-MPs) and human hemoglobin (HHb), an oxygen carrier
in the human blood circulatory system. Ultraviolet–visible
absorption studies have demonstrated that HHb molecules may interact
with PE-MPs, and thermal melting studies have indicated that PE-MPs
have a stabilizing effect on HHb. Further circular dichroism and Fourier
transform infrared spectroscopic studies have revealed the distinct
changes in HHb’s secondary structures caused by the formation
of the HHb–PE-MP binding complex. These findings imply that
PE-MPs could enter the blood circulation system of humans and may
be hazardous to humans. This work explains the potential binding interaction
of microplastics at the molecular level and offers insight into the
intermolecular interaction between PE-MPs and HHb
Quercetin Exhibits Preferential Binding Interaction by Selectively Targeting HRAS1 I‑Motif DNA-Forming Promoter Sequences
I-Motif (iM) DNA structures represent among the most
significant
noncanonical nucleic acid configurations. iM-forming DNA sequences
are found in an array of vital genomic locations and are particularly
frequent in the promoter islands of various oncogenes. Thus, iM DNA
is a crucial candidate for anticancer medicines; therefore, binding
interactions between iM DNA and small molecular ligands, such as flavonoids,
are critically important. Extensive sets of spectroscopic strategies
and thermodynamic analysis were utilized in the present investigation
to find out the favorable interaction of quercetin (Que), a dietary
flavonoid that has various health-promoting characteristics, including
anticancer properties, with noncanonical iM DNA structure. Spectroscopic
studies and thermal analysis revealed that Que interacts preferentially
with HRAS1 iM DNA compared with VEGF, BCL2 iM, and duplex DNA. Que,
therefore, emerged as a suitable natural-product-oriented antagonist
for targeting HRAS1 iM DNA. The innovative spectroscopic as well as
mechanical features of Que and its specific affinity for HRAS1 iM
may be useful for therapeutic applications and provide crucial insights
for the design of compounds with remarkable medicinal properties